Oxytocin decreases colonic motility of cold water stressed rats via oxytocin receptors

Xiao Yang, Tao Fang Xi, Yu Xian Li, Hai Hong Wang, Ying Qin, Jie Ping Zhang, Wen Ting Cai, Meng Ting Huang, Ji Qiao Shen, Xi Min Fan, Xuan-Zheng Shi, Dong Ping Xie

Research output: Contribution to journalArticle

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Abstract

AIM: To investigate whether cold water intake into the stomach affects colonic motility and the involvement of the oxytocin-oxytocin receptor pathway in rats. METHODS: Female Sprague Dawley rats were used and some of them were ovariectomized. The rats were subjected to gastric instillation with cold (0-4 °C, cold group) or room temperature (20-25 °C, control group) saline for 14 consecutive days. Colon transit was determined with a bead inserted into the colon. Colonic longitudinal muscle strips were prepared to investigate the response to oxytocin in vitro . Plasma concentration of oxytocin was detected by ELISA. Oxytocin receptor expression was investigated by Western blot analysis. Immunohistochemistry was used to locate oxytocin receptors. RESULTS: Colon transit was slower in the cold group than in the control group (P < 0.05). Colonic smooth muscle contractile response to oxytocin decreased, and the inhibitory effect of oxytocin on muscle contractility was enhanced by cold water intake (0.69 ± 0.08 vs 0.88 ± 0.16, P < 0.05). Atosiban and tetrodotoxin inhibited the effect of oxytocin on colonic motility. Oxytocin receptors were located in the myenteric plexus, and their expression was up-regulated in the cold group (P < 0.05). Cold water intake increased blood concentration of oxytocin, but this effect was attenuated in ovariectomized rats (286.99 ± 83.72 pg/mL vs 100.56 ± 92.71 pg/mL, P < 0.05). However, in ovariectomized rats, estradiol treatment increased blood oxytocin, and the response of colonic muscle strips to oxytocin was attenuated. CONCLUSION: Cold water intake inhibits colonic motility partially through oxytocin-oxytocin receptor signaling in the myenteric nervous system pathway, which is estrogen dependent.

Original languageEnglish (US)
Pages (from-to)10886-10894
Number of pages9
JournalWorld Journal of Gastroenterology
Volume20
Issue number31
DOIs
StatePublished - Aug 21 2014

Fingerprint

Oxytocin
Oxytocin Receptors
Water
Drinking
Colon
Muscles
Stomach
rat oxytocin receptors
Myenteric Plexus
Control Groups
Tetrodotoxin
Nervous System
Smooth Muscle
Sprague Dawley Rats
Estradiol
Estrogens
Western Blotting
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Temperature

Keywords

  • Colonic motility
  • Estradiol
  • Intragastric cold water stress
  • Irritable bowel syndrome
  • Oxytocin
  • Oxytocin receptor

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Yang, X., Xi, T. F., Li, Y. X., Wang, H. H., Qin, Y., Zhang, J. P., ... Xie, D. P. (2014). Oxytocin decreases colonic motility of cold water stressed rats via oxytocin receptors. World Journal of Gastroenterology, 20(31), 10886-10894. https://doi.org/10.3748/wjg.v20.i31.10886

Oxytocin decreases colonic motility of cold water stressed rats via oxytocin receptors. / Yang, Xiao; Xi, Tao Fang; Li, Yu Xian; Wang, Hai Hong; Qin, Ying; Zhang, Jie Ping; Cai, Wen Ting; Huang, Meng Ting; Shen, Ji Qiao; Fan, Xi Min; Shi, Xuan-Zheng; Xie, Dong Ping.

In: World Journal of Gastroenterology, Vol. 20, No. 31, 21.08.2014, p. 10886-10894.

Research output: Contribution to journalArticle

Yang, X, Xi, TF, Li, YX, Wang, HH, Qin, Y, Zhang, JP, Cai, WT, Huang, MT, Shen, JQ, Fan, XM, Shi, X-Z & Xie, DP 2014, 'Oxytocin decreases colonic motility of cold water stressed rats via oxytocin receptors', World Journal of Gastroenterology, vol. 20, no. 31, pp. 10886-10894. https://doi.org/10.3748/wjg.v20.i31.10886
Yang, Xiao ; Xi, Tao Fang ; Li, Yu Xian ; Wang, Hai Hong ; Qin, Ying ; Zhang, Jie Ping ; Cai, Wen Ting ; Huang, Meng Ting ; Shen, Ji Qiao ; Fan, Xi Min ; Shi, Xuan-Zheng ; Xie, Dong Ping. / Oxytocin decreases colonic motility of cold water stressed rats via oxytocin receptors. In: World Journal of Gastroenterology. 2014 ; Vol. 20, No. 31. pp. 10886-10894.
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AU - Xi, Tao Fang

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AU - Qin, Ying

AU - Zhang, Jie Ping

AU - Cai, Wen Ting

AU - Huang, Meng Ting

AU - Shen, Ji Qiao

AU - Fan, Xi Min

AU - Shi, Xuan-Zheng

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N2 - AIM: To investigate whether cold water intake into the stomach affects colonic motility and the involvement of the oxytocin-oxytocin receptor pathway in rats. METHODS: Female Sprague Dawley rats were used and some of them were ovariectomized. The rats were subjected to gastric instillation with cold (0-4 °C, cold group) or room temperature (20-25 °C, control group) saline for 14 consecutive days. Colon transit was determined with a bead inserted into the colon. Colonic longitudinal muscle strips were prepared to investigate the response to oxytocin in vitro . Plasma concentration of oxytocin was detected by ELISA. Oxytocin receptor expression was investigated by Western blot analysis. Immunohistochemistry was used to locate oxytocin receptors. RESULTS: Colon transit was slower in the cold group than in the control group (P < 0.05). Colonic smooth muscle contractile response to oxytocin decreased, and the inhibitory effect of oxytocin on muscle contractility was enhanced by cold water intake (0.69 ± 0.08 vs 0.88 ± 0.16, P < 0.05). Atosiban and tetrodotoxin inhibited the effect of oxytocin on colonic motility. Oxytocin receptors were located in the myenteric plexus, and their expression was up-regulated in the cold group (P < 0.05). Cold water intake increased blood concentration of oxytocin, but this effect was attenuated in ovariectomized rats (286.99 ± 83.72 pg/mL vs 100.56 ± 92.71 pg/mL, P < 0.05). However, in ovariectomized rats, estradiol treatment increased blood oxytocin, and the response of colonic muscle strips to oxytocin was attenuated. CONCLUSION: Cold water intake inhibits colonic motility partially through oxytocin-oxytocin receptor signaling in the myenteric nervous system pathway, which is estrogen dependent.

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KW - Irritable bowel syndrome

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