Package of Bombyx mori nuclear polyhedrosis virus with heterogoneous polyhedring protein

Ping Ji, Xinmin Zha, Guoying Zhang, Jibin Yu, Wei Gu, Weide Shen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cloning the segment of polyhedrin gene which was separated from AcMNPV genome DNA by PCR method into transfer vector pBacPAK8, we got recombinant transfer vector pOAc, then contransfecting BmN cells with the vector and linear virus Bm-BacPAK6, harvested recombinant virus hp-BmNPV which can produce polyhedrin but no blue plaques. Analysing of recombinant virus from polyhedrin, recombinant viral DNA and polyhedra, we confirmed that polyhedring of AcMNPV can be not only high-level expressed in BmN cells, But can recognize recombinant BmNPV DNA and assemble them to polyhedra. We observed that the pattern of restriction enzyme changed due to DNA recombination, the size of recombinant viral polyhedron (1.2-2.9 microns) was obviously smaller than those of wild-type BmNPV by electron microscopy.

Original languageEnglish (US)
Pages (from-to)50-55
Number of pages6
JournalWei sheng wu xue bao = Acta microbiologica Sinica
Volume42
Issue number1
StatePublished - 2002
Externally publishedYes

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Nucleopolyhedrovirus
Bombyx
Recombinant DNA
Viruses
Proteins
DNA
Viral DNA
Genetic Recombination
Organism Cloning
Electron Microscopy
Genome
Polymerase Chain Reaction
Enzymes
Genes

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Package of Bombyx mori nuclear polyhedrosis virus with heterogoneous polyhedring protein. / Ji, Ping; Zha, Xinmin; Zhang, Guoying; Yu, Jibin; Gu, Wei; Shen, Weide.

In: Wei sheng wu xue bao = Acta microbiologica Sinica, Vol. 42, No. 1, 2002, p. 50-55.

Research output: Contribution to journalArticle

Ji, Ping ; Zha, Xinmin ; Zhang, Guoying ; Yu, Jibin ; Gu, Wei ; Shen, Weide. / Package of Bombyx mori nuclear polyhedrosis virus with heterogoneous polyhedring protein. In: Wei sheng wu xue bao = Acta microbiologica Sinica. 2002 ; Vol. 42, No. 1. pp. 50-55.
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AU - Shen, Weide

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