Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord

David Cabañero, Alyssa Baker, Shengtai Zhou, Gregory L. Hargett, Takeshi Irie, Yan Xia, Hélène Beaudry, Louis Gendron, Zara Melyan, Susan M. Carlton, Jose A. Morón

    Research output: Contribution to journalArticle

    12 Scopus citations

    Abstract

    Withdrawal from prescribed opioids results in increased pain sensitivity, which prolongs the treatment. This pain sensitivity is attributed to neuroplastic changes that converge at the spinal cord dorsal horn. We have recently reported that repeated morphine administration triggers an insertion of GluA2-lacking (Ca2+ -permeable) α-amino-3-hydroxy-5-methyl-4- isoxazole propionic acid receptors (AMPAR) in the hippocampus. This finding together with the reported involvement of AMPAR in the mechanisms underlying inflammatory pain led us to hypothesize a role for spinal AMPAR in opioid-induced pain behavior. Mice treated with escalating doses of morphine showed hypersensitivity to mechanical stimulation. Intrathecal administration of a Ca2+ -permeable AMPAR selective blocker disrupted morphine-induced mechanical sensitivity. Analysis of the expression and phosphorylation levels of AMPAR subunits (GluA1/2/3/4) in homogenates and in postsynaptic density fractions from spinal cord dorsal horns showed an increase in GluA4 expression and phosphorylation in the postsynaptic density after morphine. Co-immunoprecipitation analyses suggested an increase in GluA4 homomers (Ca 2+ -permeable AMPAR) and immunohistochemical staining localized the increase in GluA4 levels in laminae III-V. The excitatory postsynaptic currents (EPSCs) recorded in laminae III-V showed enhanced sensitivity to Ca2+ -permeable AMPAR blockers in morphine-treated mice. Furthermore, current-voltage relationships of AMPAR-mediated EPSCs showed that rectification index (an indicator of Ca2+ -permeable AMPAR contribution) is increased in morphine-treated but not in saline-treated mice. These effects could be reversed by infusion of GluA4 antibody through patch pipette. This is the first direct evidence for a role of GluA4-containing AMPAR in morphine-induced pain and highlights spinal GluA4-containing AMPAR as targets to prevent the morphine-induced pain sensitivity.

    Original languageEnglish (US)
    Pages (from-to)1472-1484
    Number of pages13
    JournalNeuropsychopharmacology
    Volume38
    Issue number8
    DOIs
    StatePublished - Jul 2013

    Keywords

    • AMPAR
    • GluA4
    • Morphine
    • pain
    • spinal cord
    • synapse

    ASJC Scopus subject areas

    • Pharmacology
    • Psychiatry and Mental health

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  • Cite this

    Cabañero, D., Baker, A., Zhou, S., Hargett, G. L., Irie, T., Xia, Y., Beaudry, H., Gendron, L., Melyan, Z., Carlton, S. M., & Morón, J. A. (2013). Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord. Neuropsychopharmacology, 38(8), 1472-1484. https://doi.org/10.1038/npp.2013.46