Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord

David Cabañero, Alyssa Baker, Shengtai Zhou, Gregory L. Hargett, Takeshi Irie, Yan Xia, Hélène Beaudry, Louis Gendron, Zara Melyan, Susan M. Carlton, Jose A. Morón

Research output: Contribution to journalArticle

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Abstract

Withdrawal from prescribed opioids results in increased pain sensitivity, which prolongs the treatment. This pain sensitivity is attributed to neuroplastic changes that converge at the spinal cord dorsal horn. We have recently reported that repeated morphine administration triggers an insertion of GluA2-lacking (Ca2+ -permeable) α-amino-3-hydroxy-5-methyl-4- isoxazole propionic acid receptors (AMPAR) in the hippocampus. This finding together with the reported involvement of AMPAR in the mechanisms underlying inflammatory pain led us to hypothesize a role for spinal AMPAR in opioid-induced pain behavior. Mice treated with escalating doses of morphine showed hypersensitivity to mechanical stimulation. Intrathecal administration of a Ca2+ -permeable AMPAR selective blocker disrupted morphine-induced mechanical sensitivity. Analysis of the expression and phosphorylation levels of AMPAR subunits (GluA1/2/3/4) in homogenates and in postsynaptic density fractions from spinal cord dorsal horns showed an increase in GluA4 expression and phosphorylation in the postsynaptic density after morphine. Co-immunoprecipitation analyses suggested an increase in GluA4 homomers (Ca 2+ -permeable AMPAR) and immunohistochemical staining localized the increase in GluA4 levels in laminae III-V. The excitatory postsynaptic currents (EPSCs) recorded in laminae III-V showed enhanced sensitivity to Ca2+ -permeable AMPAR blockers in morphine-treated mice. Furthermore, current-voltage relationships of AMPAR-mediated EPSCs showed that rectification index (an indicator of Ca2+ -permeable AMPAR contribution) is increased in morphine-treated but not in saline-treated mice. These effects could be reversed by infusion of GluA4 antibody through patch pipette. This is the first direct evidence for a role of GluA4-containing AMPAR in morphine-induced pain and highlights spinal GluA4-containing AMPAR as targets to prevent the morphine-induced pain sensitivity.

Original languageEnglish (US)
Pages (from-to)1472-1484
Number of pages13
JournalNeuropsychopharmacology
Volume38
Issue number8
DOIs
StatePublished - Jul 2013

Fingerprint

Isoxazoles
Morphine
Pain
Therapeutics
Post-Synaptic Density
Excitatory Postsynaptic Potentials
propionic acid
Spinal Cord Dorsal Horn
Phosphorylation
mu Opioid Receptor
Opioid Receptors
Immunoprecipitation
Opioid Analgesics
Hippocampus
Hypersensitivity

Keywords

  • AMPAR
  • GluA4
  • Morphine
  • pain
  • spinal cord
  • synapse

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health

Cite this

Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord. / Cabañero, David; Baker, Alyssa; Zhou, Shengtai; Hargett, Gregory L.; Irie, Takeshi; Xia, Yan; Beaudry, Hélène; Gendron, Louis; Melyan, Zara; Carlton, Susan M.; Morón, Jose A.

In: Neuropsychopharmacology, Vol. 38, No. 8, 07.2013, p. 1472-1484.

Research output: Contribution to journalArticle

Cabañero, D, Baker, A, Zhou, S, Hargett, GL, Irie, T, Xia, Y, Beaudry, H, Gendron, L, Melyan, Z, Carlton, SM & Morón, JA 2013, 'Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord', Neuropsychopharmacology, vol. 38, no. 8, pp. 1472-1484. https://doi.org/10.1038/npp.2013.46
Cabañero, David ; Baker, Alyssa ; Zhou, Shengtai ; Hargett, Gregory L. ; Irie, Takeshi ; Xia, Yan ; Beaudry, Hélène ; Gendron, Louis ; Melyan, Zara ; Carlton, Susan M. ; Morón, Jose A. / Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord. In: Neuropsychopharmacology. 2013 ; Vol. 38, No. 8. pp. 1472-1484.
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