Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation

G. L. Lyford, C. L. He, E. Soffer, T. L. Hull, S. A. Strong, A. J. Senagore, L. J. Burgart, T. Young-Fadok, J. H. Szurszewski, Gianrico Farrugia

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

Background: Interstitial cells of Cajal (ICC) are required for normal intestinal motility. ICC are found throughout the human colon and are decreased in the sigmoid colon of patients with slow transit constipation. Aims: The aims of this study were to determine the normal distribution of ICC within the human colon and to determine if ICC are decreased throughout the colon in slow transit constipation. Patients: The caecum, ascending, transverse, and sigmoid colons from six patients with slow transit constipation and colonic tissue from patients with resected colon cancer were used for this study. Methods: ICC cells were identified with a polyclonal antibody to c-Kit, serial 0.5 μm sections were obtained by confocal microscopy, and three dimensional software was employed to reconstruct the entire thickness of the colonic muscularis propria and submucosa. Results: ICC were located within both the longitudinal and circular muscle layers. Two networks of ICC were identified, one in the myenteric plexus region and another, less defined network, in the submucosal border. Caecum, ascending colon, transverse colon, and sigmoid colon displayed similar ICC volumes. ICC volume was significantly lower in the slow transit constipation potients across all colonic regions. Conclusions: The data suggest that ICC distribution is relatively uniform throughout the human colon and that decreased ICC volume is pan-colonic in idiopathic slow transit constipation.

Original languageEnglish (US)
Pages (from-to)496-501
Number of pages6
JournalGut
Volume51
Issue number4
DOIs
StatePublished - Oct 2002
Externally publishedYes

Fingerprint

Interstitial Cells of Cajal
Constipation
Colon
Sigmoid Colon
Cell Size
Ascending Colon
Transverse Colon
Myenteric Plexus
Gastrointestinal Motility
Normal Distribution
Confocal Microscopy
Colonic Neoplasms
Software

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Lyford, G. L., He, C. L., Soffer, E., Hull, T. L., Strong, S. A., Senagore, A. J., ... Farrugia, G. (2002). Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation. Gut, 51(4), 496-501. https://doi.org/10.1136/gut.51.4.496

Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation. / Lyford, G. L.; He, C. L.; Soffer, E.; Hull, T. L.; Strong, S. A.; Senagore, A. J.; Burgart, L. J.; Young-Fadok, T.; Szurszewski, J. H.; Farrugia, Gianrico.

In: Gut, Vol. 51, No. 4, 10.2002, p. 496-501.

Research output: Contribution to journalArticle

Lyford, GL, He, CL, Soffer, E, Hull, TL, Strong, SA, Senagore, AJ, Burgart, LJ, Young-Fadok, T, Szurszewski, JH & Farrugia, G 2002, 'Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation', Gut, vol. 51, no. 4, pp. 496-501. https://doi.org/10.1136/gut.51.4.496
Lyford GL, He CL, Soffer E, Hull TL, Strong SA, Senagore AJ et al. Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation. Gut. 2002 Oct;51(4):496-501. https://doi.org/10.1136/gut.51.4.496
Lyford, G. L. ; He, C. L. ; Soffer, E. ; Hull, T. L. ; Strong, S. A. ; Senagore, A. J. ; Burgart, L. J. ; Young-Fadok, T. ; Szurszewski, J. H. ; Farrugia, Gianrico. / Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation. In: Gut. 2002 ; Vol. 51, No. 4. pp. 496-501.
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AU - Strong, S. A.

AU - Senagore, A. J.

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