Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol

J. Werner, Michael Laposata, C. Fernandez-Del Castillo, M. Saghir, R. V. Iozzo, K. B. Lewandrowski, A. L. Warshaw

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Background and Aims: The mechanism by which alcohol injures the pancreas remains unknown. Alcohol-intoxicated humans have high levels of fatty acid ethyl esters (FAEEs), nonoxidative products of ethanol metabolism, in blood, pancreas, and liver. The aims of this study were to determine whether FAEEs are toxic to the pancreas in vivo and, if so, to assess whether this injury is specific to the pancreas and to compare it to the injury observed in acute pancreatitis. Methods: FAEEs were infused into Sprague-Dawley rats. Levels of FAEEs in plasma and pancreas were measured, and pancreatic injury was assessed during a 48-hour period for edema formation and ectopic trypsinogen activation and by light and electron microscopy. Results: FAEEs induced highly significant increases in pancreatic edema, pancreatic trypsinogen activation, and vacuolization of acinar cells. These findings were specific to the pancreas and were not found in liver, lung, myocardium, skeletal muscle, or subcutaneous fat. Conclusions: FAEEs at concentrations found in human plasma produce a pancreatitis-like injury in rats, providing direct evidence that FAEEs can produce organ-specific toxicity. Thus, FAEEs may contribute to acute alcohol-induced damage to the pancreas.

Original languageEnglish (US)
Pages (from-to)286-294
Number of pages9
JournalGastroenterology
Volume113
Issue number1
DOIs
StatePublished - 1997
Externally publishedYes

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Esters
Fatty Acids
Pancreas
Alcohols
Wounds and Injuries
Trypsinogen
Pancreatitis
Edema
Acinar Cells
Poisons
Subcutaneous Fat
Liver
Sprague Dawley Rats
Myocardium
Electron Microscopy
Skeletal Muscle
Ethanol
Light
Lung

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Werner, J., Laposata, M., Fernandez-Del Castillo, C., Saghir, M., Iozzo, R. V., Lewandrowski, K. B., & Warshaw, A. L. (1997). Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol. Gastroenterology, 113(1), 286-294. https://doi.org/10.1016/S0016-5085(97)70106-9

Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol. / Werner, J.; Laposata, Michael; Fernandez-Del Castillo, C.; Saghir, M.; Iozzo, R. V.; Lewandrowski, K. B.; Warshaw, A. L.

In: Gastroenterology, Vol. 113, No. 1, 1997, p. 286-294.

Research output: Contribution to journalArticle

Werner, J, Laposata, M, Fernandez-Del Castillo, C, Saghir, M, Iozzo, RV, Lewandrowski, KB & Warshaw, AL 1997, 'Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol', Gastroenterology, vol. 113, no. 1, pp. 286-294. https://doi.org/10.1016/S0016-5085(97)70106-9
Werner, J. ; Laposata, Michael ; Fernandez-Del Castillo, C. ; Saghir, M. ; Iozzo, R. V. ; Lewandrowski, K. B. ; Warshaw, A. L. / Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol. In: Gastroenterology. 1997 ; Vol. 113, No. 1. pp. 286-294.
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