Pancreatic islets activate portal vein endothelial cells in vitro

Magali J. Fontaine, Jacqueline Blanchard, Cristiana Rastellini, Velda Lazda, Kevan C. Herold, Raymond Pollak

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study assessed the active role of the portal vein endothelium in the functional loss of pancreatic islet (PI) grafts, considered in the context of PI transplantation for treatment of type I diabetes mellitus. We hypothesized that PI engraftment may be jeopardized by portal vein endothelial cell-induced activation of host T cells. We designed an in vitro system using portal vein endothelial cells (PVEC) from Lewis (Lew) rats and PI from Brown-Norway (BN) and Lew rats. PI were co-cultured with Lew PVEC for three days. The PI were removed and trypsinized PVEC were divided into three groups: (A) PVEC not exposed to PI; (B) PVEC exposed to syngeneic PI; and (C) PVEC exposed to allogeneic PI. The groups were analyzed by flow cytometry for ICAM-1 and MHC Class I and Class II molecules. Functional assays of lymphocytes (ie, lymphocyte proliferation assay, 51Cr release assay, and cytokine release assay) were also performed. We observed MHC Class I and ICAM-1 upregulation on PVEC after PI contact. MHC Class II molecule was not upregulated on PVEC after PI exposure. IL-6 production was increased non-specifically following PI coculture with PVEC. TNF-α was increased only after allogeneic PI-PVEC coculture. PVEC exposed to either allogeneic or syngeneic PI could not stimulate naïve splenocyte proliferation or cytotoxicity. We conclude that PVEC allo-PI interaction results in increased ICAM-1 and MHC Class I expression on the PVEC. Neither lymphocyte proliferation nor cytotoxicity could be enhanced in response to enhanced MHC Class I and ICAM-1 expression, which was associated instead with non-specific inflammatory cytokine release.

Original languageEnglish (US)
Pages (from-to)352-362
Number of pages11
JournalAnnals of Clinical and Laboratory Science
Volume32
Issue number4
StatePublished - Sep 2002
Externally publishedYes

Fingerprint

Endothelial cells
Portal Vein
Islets of Langerhans
Endothelial Cells
Intercellular Adhesion Molecule-1
Lymphocytes
Assays
In Vitro Techniques
Cytotoxicity
Coculture Techniques
Rats
Cytokines
Transplantation (surgical)
Islets of Langerhans Transplantation
Molecules
T-cells
Flow cytometry
Medical problems
Type 1 Diabetes Mellitus
Grafts

Keywords

  • Antigen presentation
  • Inflammatory cytokines
  • Pancreatic islets
  • Portal vein endothelium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

Fontaine, M. J., Blanchard, J., Rastellini, C., Lazda, V., Herold, K. C., & Pollak, R. (2002). Pancreatic islets activate portal vein endothelial cells in vitro. Annals of Clinical and Laboratory Science, 32(4), 352-362.

Pancreatic islets activate portal vein endothelial cells in vitro. / Fontaine, Magali J.; Blanchard, Jacqueline; Rastellini, Cristiana; Lazda, Velda; Herold, Kevan C.; Pollak, Raymond.

In: Annals of Clinical and Laboratory Science, Vol. 32, No. 4, 09.2002, p. 352-362.

Research output: Contribution to journalArticle

Fontaine, MJ, Blanchard, J, Rastellini, C, Lazda, V, Herold, KC & Pollak, R 2002, 'Pancreatic islets activate portal vein endothelial cells in vitro', Annals of Clinical and Laboratory Science, vol. 32, no. 4, pp. 352-362.
Fontaine MJ, Blanchard J, Rastellini C, Lazda V, Herold KC, Pollak R. Pancreatic islets activate portal vein endothelial cells in vitro. Annals of Clinical and Laboratory Science. 2002 Sep;32(4):352-362.
Fontaine, Magali J. ; Blanchard, Jacqueline ; Rastellini, Cristiana ; Lazda, Velda ; Herold, Kevan C. ; Pollak, Raymond. / Pancreatic islets activate portal vein endothelial cells in vitro. In: Annals of Clinical and Laboratory Science. 2002 ; Vol. 32, No. 4. pp. 352-362.
@article{97e3023ed7ae4a7b96a91db919c046df,
title = "Pancreatic islets activate portal vein endothelial cells in vitro",
abstract = "This study assessed the active role of the portal vein endothelium in the functional loss of pancreatic islet (PI) grafts, considered in the context of PI transplantation for treatment of type I diabetes mellitus. We hypothesized that PI engraftment may be jeopardized by portal vein endothelial cell-induced activation of host T cells. We designed an in vitro system using portal vein endothelial cells (PVEC) from Lewis (Lew) rats and PI from Brown-Norway (BN) and Lew rats. PI were co-cultured with Lew PVEC for three days. The PI were removed and trypsinized PVEC were divided into three groups: (A) PVEC not exposed to PI; (B) PVEC exposed to syngeneic PI; and (C) PVEC exposed to allogeneic PI. The groups were analyzed by flow cytometry for ICAM-1 and MHC Class I and Class II molecules. Functional assays of lymphocytes (ie, lymphocyte proliferation assay, 51Cr release assay, and cytokine release assay) were also performed. We observed MHC Class I and ICAM-1 upregulation on PVEC after PI contact. MHC Class II molecule was not upregulated on PVEC after PI exposure. IL-6 production was increased non-specifically following PI coculture with PVEC. TNF-α was increased only after allogeneic PI-PVEC coculture. PVEC exposed to either allogeneic or syngeneic PI could not stimulate na{\"i}ve splenocyte proliferation or cytotoxicity. We conclude that PVEC allo-PI interaction results in increased ICAM-1 and MHC Class I expression on the PVEC. Neither lymphocyte proliferation nor cytotoxicity could be enhanced in response to enhanced MHC Class I and ICAM-1 expression, which was associated instead with non-specific inflammatory cytokine release.",
keywords = "Antigen presentation, Inflammatory cytokines, Pancreatic islets, Portal vein endothelium",
author = "Fontaine, {Magali J.} and Jacqueline Blanchard and Cristiana Rastellini and Velda Lazda and Herold, {Kevan C.} and Raymond Pollak",
year = "2002",
month = "9",
language = "English (US)",
volume = "32",
pages = "352--362",
journal = "Annals of Clinical and Laboratory Science",
issn = "0091-7370",
publisher = "Association of Clinical Scientists",
number = "4",

}

TY - JOUR

T1 - Pancreatic islets activate portal vein endothelial cells in vitro

AU - Fontaine, Magali J.

AU - Blanchard, Jacqueline

AU - Rastellini, Cristiana

AU - Lazda, Velda

AU - Herold, Kevan C.

AU - Pollak, Raymond

PY - 2002/9

Y1 - 2002/9

N2 - This study assessed the active role of the portal vein endothelium in the functional loss of pancreatic islet (PI) grafts, considered in the context of PI transplantation for treatment of type I diabetes mellitus. We hypothesized that PI engraftment may be jeopardized by portal vein endothelial cell-induced activation of host T cells. We designed an in vitro system using portal vein endothelial cells (PVEC) from Lewis (Lew) rats and PI from Brown-Norway (BN) and Lew rats. PI were co-cultured with Lew PVEC for three days. The PI were removed and trypsinized PVEC were divided into three groups: (A) PVEC not exposed to PI; (B) PVEC exposed to syngeneic PI; and (C) PVEC exposed to allogeneic PI. The groups were analyzed by flow cytometry for ICAM-1 and MHC Class I and Class II molecules. Functional assays of lymphocytes (ie, lymphocyte proliferation assay, 51Cr release assay, and cytokine release assay) were also performed. We observed MHC Class I and ICAM-1 upregulation on PVEC after PI contact. MHC Class II molecule was not upregulated on PVEC after PI exposure. IL-6 production was increased non-specifically following PI coculture with PVEC. TNF-α was increased only after allogeneic PI-PVEC coculture. PVEC exposed to either allogeneic or syngeneic PI could not stimulate naïve splenocyte proliferation or cytotoxicity. We conclude that PVEC allo-PI interaction results in increased ICAM-1 and MHC Class I expression on the PVEC. Neither lymphocyte proliferation nor cytotoxicity could be enhanced in response to enhanced MHC Class I and ICAM-1 expression, which was associated instead with non-specific inflammatory cytokine release.

AB - This study assessed the active role of the portal vein endothelium in the functional loss of pancreatic islet (PI) grafts, considered in the context of PI transplantation for treatment of type I diabetes mellitus. We hypothesized that PI engraftment may be jeopardized by portal vein endothelial cell-induced activation of host T cells. We designed an in vitro system using portal vein endothelial cells (PVEC) from Lewis (Lew) rats and PI from Brown-Norway (BN) and Lew rats. PI were co-cultured with Lew PVEC for three days. The PI were removed and trypsinized PVEC were divided into three groups: (A) PVEC not exposed to PI; (B) PVEC exposed to syngeneic PI; and (C) PVEC exposed to allogeneic PI. The groups were analyzed by flow cytometry for ICAM-1 and MHC Class I and Class II molecules. Functional assays of lymphocytes (ie, lymphocyte proliferation assay, 51Cr release assay, and cytokine release assay) were also performed. We observed MHC Class I and ICAM-1 upregulation on PVEC after PI contact. MHC Class II molecule was not upregulated on PVEC after PI exposure. IL-6 production was increased non-specifically following PI coculture with PVEC. TNF-α was increased only after allogeneic PI-PVEC coculture. PVEC exposed to either allogeneic or syngeneic PI could not stimulate naïve splenocyte proliferation or cytotoxicity. We conclude that PVEC allo-PI interaction results in increased ICAM-1 and MHC Class I expression on the PVEC. Neither lymphocyte proliferation nor cytotoxicity could be enhanced in response to enhanced MHC Class I and ICAM-1 expression, which was associated instead with non-specific inflammatory cytokine release.

KW - Antigen presentation

KW - Inflammatory cytokines

KW - Pancreatic islets

KW - Portal vein endothelium

UR - http://www.scopus.com/inward/record.url?scp=0036755564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036755564&partnerID=8YFLogxK

M3 - Article

VL - 32

SP - 352

EP - 362

JO - Annals of Clinical and Laboratory Science

JF - Annals of Clinical and Laboratory Science

SN - 0091-7370

IS - 4

ER -