Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases

Gustavo Palacios, Phenix Lan Quan, Omar J. Jabado, Sean Conlan, David L. Hirschberg, Yang Liu, Junhui Zhai, Neil Renwick, Jeffrey Hui, Hedi Hegyi, Allen Grolla, James E. Strong, Jonathan S. Towner, Thomas Geisbert, Peter B. Jahrling, Cornelia Büchen-Osmond, Heinz Ellerbrok, Maria Paz Sanchez-Seco, Yves Lussier, Pierre Formenty & 4 others Stuart T. Nichol, Heinz Feldmann, Thomas Briese, W. Ian Lipkin

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.

Original languageEnglish (US)
Pages (from-to)73-81
Number of pages9
JournalEmerging Infectious Diseases
Volume13
Issue number1
StatePublished - Jan 2007
Externally publishedYes

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Oligonucleotide Array Sequence Analysis
Nucleic Acids
Communicable Diseases
Marburg Virus Disease
Angola
Viruses
Bacteria
Oligonucleotide Probes
Plasmodium falciparum
Disease Outbreaks
Vertebrates
Parasites
Differential Diagnosis
Fungi
Urine
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Palacios, G., Quan, P. L., Jabado, O. J., Conlan, S., Hirschberg, D. L., Liu, Y., ... Lipkin, W. I. (2007). Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases. Emerging Infectious Diseases, 13(1), 73-81.

Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases. / Palacios, Gustavo; Quan, Phenix Lan; Jabado, Omar J.; Conlan, Sean; Hirschberg, David L.; Liu, Yang; Zhai, Junhui; Renwick, Neil; Hui, Jeffrey; Hegyi, Hedi; Grolla, Allen; Strong, James E.; Towner, Jonathan S.; Geisbert, Thomas; Jahrling, Peter B.; Büchen-Osmond, Cornelia; Ellerbrok, Heinz; Sanchez-Seco, Maria Paz; Lussier, Yves; Formenty, Pierre; Nichol, Stuart T.; Feldmann, Heinz; Briese, Thomas; Lipkin, W. Ian.

In: Emerging Infectious Diseases, Vol. 13, No. 1, 01.2007, p. 73-81.

Research output: Contribution to journalArticle

Palacios, G, Quan, PL, Jabado, OJ, Conlan, S, Hirschberg, DL, Liu, Y, Zhai, J, Renwick, N, Hui, J, Hegyi, H, Grolla, A, Strong, JE, Towner, JS, Geisbert, T, Jahrling, PB, Büchen-Osmond, C, Ellerbrok, H, Sanchez-Seco, MP, Lussier, Y, Formenty, P, Nichol, ST, Feldmann, H, Briese, T & Lipkin, WI 2007, 'Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases', Emerging Infectious Diseases, vol. 13, no. 1, pp. 73-81.
Palacios G, Quan PL, Jabado OJ, Conlan S, Hirschberg DL, Liu Y et al. Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases. Emerging Infectious Diseases. 2007 Jan;13(1):73-81.
Palacios, Gustavo ; Quan, Phenix Lan ; Jabado, Omar J. ; Conlan, Sean ; Hirschberg, David L. ; Liu, Yang ; Zhai, Junhui ; Renwick, Neil ; Hui, Jeffrey ; Hegyi, Hedi ; Grolla, Allen ; Strong, James E. ; Towner, Jonathan S. ; Geisbert, Thomas ; Jahrling, Peter B. ; Büchen-Osmond, Cornelia ; Ellerbrok, Heinz ; Sanchez-Seco, Maria Paz ; Lussier, Yves ; Formenty, Pierre ; Nichol, Stuart T. ; Feldmann, Heinz ; Briese, Thomas ; Lipkin, W. Ian. / Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases. In: Emerging Infectious Diseases. 2007 ; Vol. 13, No. 1. pp. 73-81.
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