Pannexin1 hemichannels are critical for HIV infection of human primary CD4+ T lymphocytes

J. A. Orellana, S. Velasquez, D. W. Williams, J. C. Sáez, J. W. Berman, Eliseo Eugenin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

HIV is a major public health issue, and infection of CD4+ T lymphocytes is one of its key features. Whereas several cellular proteins have been identified that facilitate viral infection and replication, the role of hemichannels in these processes has not been fully characterized. We now show that the HIV isolates, R5 and X4, induced a transient-early (5-30 min) and a later, persistent (48-120 h) opening of Panx1 hemichannels, which was dependent on the binding of HIV to CD4 and CCR5/CXCR4 receptors. Blocking Panx1 hemichannels by reducing their opening or protein expression inhibited HIV replication in CD4+ T lymphocytes. Thus, our findings demonstrate that Panx1 hemichannels play an essential role in HIV infection.

Original languageEnglish (US)
Pages (from-to)399-407
Number of pages9
JournalJournal of Leukocyte Biology
Volume94
Issue number3
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

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HIV Infections
HIV
T-Lymphocytes
CCR5 Receptors
CXCR4 Receptors
Virus Diseases
Proteins
Public Health
Infection

Keywords

  • AIDS
  • Channels
  • Disease

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Pannexin1 hemichannels are critical for HIV infection of human primary CD4+ T lymphocytes. / Orellana, J. A.; Velasquez, S.; Williams, D. W.; Sáez, J. C.; Berman, J. W.; Eugenin, Eliseo.

In: Journal of Leukocyte Biology, Vol. 94, No. 3, 01.09.2013, p. 399-407.

Research output: Contribution to journalArticle

Orellana, J. A. ; Velasquez, S. ; Williams, D. W. ; Sáez, J. C. ; Berman, J. W. ; Eugenin, Eliseo. / Pannexin1 hemichannels are critical for HIV infection of human primary CD4+ T lymphocytes. In: Journal of Leukocyte Biology. 2013 ; Vol. 94, No. 3. pp. 399-407.
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