TY - JOUR
T1 - Paramyxoviruses from neotropical bats suggest a novel genus and nephrotropism
AU - de Souza, William Marciel
AU - Fumagalli, Marcilio Jorge
AU - Carrera, Jean Paul
AU - de Araujo, Jansen
AU - Cardoso, Jedson Ferreira
AU - de Carvalho, Cristiano
AU - Durigon, Edison Luiz
AU - Queiroz, Luzia Helena
AU - Faria, Nuno Rodrigues
AU - Murcia, Pablo R.
AU - Figueiredo, Luiz Tadeu Moraes
N1 - Funding Information:
This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo , Brazil (Grant number. 13/14929-1 , and Scholarships No. 06/00572-0 , 15/05778-5 ; 17/13981-0 , 19/24251-9 and 18/09383-3 ) and by the CADDE/FAPESP Brazil-UK partnership grant ( MR/S0195/1 and FAPESP 18/14389-0 ) ( http://caddecentre.org/ ). NRF is supported by a Wellcome Trust and Royal Society Sir Henry Dale Fellowship ( 204311/Z/16/Z ). PRM is supported by the Medical Research Council of the United Kingdom ( MC_UU_12014/9 ). National Institutes of Health grant R24 AI120942 .
Publisher Copyright:
© 2021
PY - 2021/11
Y1 - 2021/11
N2 - Paramyxoviruses have a broad host range and geographic distribution, including human pathogens transmitted by bats, such as Nipah and Hendra viruses. In this study, we combined high-throughput sequencing and molecular approaches to investigate the presence of paramyxoviruses in neotropical bats (Microchiroptera suborder) in Brazil. We discovered and characterized three novel paramyxoviruses in the kidney tissues of apparently healthy common vampire bats (D. rotundus) and Seba's short-tailed bats (C. perspicillata), which we tentatively named Kanhgág virus (KANV), Boe virus (BOEV), and Guató virus (GUATV). In this study, we classified these viruses as putative species into the Macrojêvirus genus, a newly proposed genus of the Orthoparamyxovirinae subfamily. Using RT-PCR, we detected these viruses in 20.9% (9 out of 43) of bats tested, and viral RNA was detected exclusively in kidney tissues. Attempts to isolate infectious virus were successful for KANV and GUATV. Our results expand the viral diversity, host range, and geographical distribution of the paramyxoviruses.
AB - Paramyxoviruses have a broad host range and geographic distribution, including human pathogens transmitted by bats, such as Nipah and Hendra viruses. In this study, we combined high-throughput sequencing and molecular approaches to investigate the presence of paramyxoviruses in neotropical bats (Microchiroptera suborder) in Brazil. We discovered and characterized three novel paramyxoviruses in the kidney tissues of apparently healthy common vampire bats (D. rotundus) and Seba's short-tailed bats (C. perspicillata), which we tentatively named Kanhgág virus (KANV), Boe virus (BOEV), and Guató virus (GUATV). In this study, we classified these viruses as putative species into the Macrojêvirus genus, a newly proposed genus of the Orthoparamyxovirinae subfamily. Using RT-PCR, we detected these viruses in 20.9% (9 out of 43) of bats tested, and viral RNA was detected exclusively in kidney tissues. Attempts to isolate infectious virus were successful for KANV and GUATV. Our results expand the viral diversity, host range, and geographical distribution of the paramyxoviruses.
KW - Bat-borne diseases
KW - Paramyxoviridae
KW - Paramyxovirus
KW - Virus discovery
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U2 - 10.1016/j.meegid.2021.105041
DO - 10.1016/j.meegid.2021.105041
M3 - Article
C2 - 34411742
AN - SCOPUS:85113230171
VL - 95
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
M1 - 105041
ER -