Parenteral glutathione monoester enhances tissue antioxidant stores

M. K. Robinson, M. S. Ahn, J. D. Rounds, J. A. Cook, D. O. Jacobs, D. W. Wilmore

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Glutathione (GSH) is a potent endogenous antioxidant that protects major organs from oxidant injury. However, present nutrition regimens may inadequately support tissue stores of this tripeptide during critical illness. To determine whether GSH reserves can be enhanced in vivo with intravenous (IV) supplements, rats underwent central venous catheterization, were given chow and water ad libitum during a 2-day recovery period, and were then randomized to receive one of three treatments as an IV bolus: (1) dextrose, (2) glutathione (GSH), or (3) glutathione monoethyl ester. GSH monoethyl ester is transported into cells more easily than is GSH. Tissue and plasma samples were analyzed for GSH at 2 and 4 hours after drug administration. Liver, renal, and ileal mucosal GSH were significantly increased in the GSH-monoethyl ester rats compared with dextrose-treated animals. In addition, plasma GSH was dramatically increased after monoester injection. In contrast, GSH administration depressed liver GSH stores and did not significantly affect GSH concentration in the other organs analyzed. Plasma GSH concentration was elevated 2 hours after GSH administration. We conclude that: (1) the monoethyl ester of glutathione can be used in vivo to enhance tissue and plasma GSH concentration and (2) IV GSH administration does not significantly increase tissue GSH levels and may paradoxically depress hepatic GSH in normal rats. Because the malnourished and critically ill are likely to have depleted GSH stores, nutrition strategies that include the provision of GSH monoester may lend additional support to those organs that are at risk for injury from oxygen free radicals during catabolic states.

Original languageEnglish (US)
Pages (from-to)413-418
Number of pages6
JournalJournal of Parenteral and Enteral Nutrition
Volume16
Issue number5
StatePublished - 1992
Externally publishedYes

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Glutathione
glutathione
Antioxidants
esters
antioxidants
Critical Illness
liver
Liver
rats
Esters
Central Venous Catheterization
nutrition
Organs at Risk
Glucose
tripeptides
glucose
Wounds and Injuries
Oxidants
catheters
oxidants

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Robinson, M. K., Ahn, M. S., Rounds, J. D., Cook, J. A., Jacobs, D. O., & Wilmore, D. W. (1992). Parenteral glutathione monoester enhances tissue antioxidant stores. Journal of Parenteral and Enteral Nutrition, 16(5), 413-418.

Parenteral glutathione monoester enhances tissue antioxidant stores. / Robinson, M. K.; Ahn, M. S.; Rounds, J. D.; Cook, J. A.; Jacobs, D. O.; Wilmore, D. W.

In: Journal of Parenteral and Enteral Nutrition, Vol. 16, No. 5, 1992, p. 413-418.

Research output: Contribution to journalArticle

Robinson, MK, Ahn, MS, Rounds, JD, Cook, JA, Jacobs, DO & Wilmore, DW 1992, 'Parenteral glutathione monoester enhances tissue antioxidant stores', Journal of Parenteral and Enteral Nutrition, vol. 16, no. 5, pp. 413-418.
Robinson MK, Ahn MS, Rounds JD, Cook JA, Jacobs DO, Wilmore DW. Parenteral glutathione monoester enhances tissue antioxidant stores. Journal of Parenteral and Enteral Nutrition. 1992;16(5):413-418.
Robinson, M. K. ; Ahn, M. S. ; Rounds, J. D. ; Cook, J. A. ; Jacobs, D. O. ; Wilmore, D. W. / Parenteral glutathione monoester enhances tissue antioxidant stores. In: Journal of Parenteral and Enteral Nutrition. 1992 ; Vol. 16, No. 5. pp. 413-418.
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