Partial genetic characterization of West Nile virus strains, New York State, 2000

Gregory D. Ebel, Alan P. Dupuis, Kiet Ngo, David Nicholas, Elizabeth Kauffman, Susan A. Jones, Donna Young, Joseph Maffei, Pei-Yong Shi, Kristen Bernard, Laura D. Kramer

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.

Original languageEnglish (US)
Pages (from-to)650-653
Number of pages4
JournalEmerging Infectious Diseases
Volume7
Issue number4
StatePublished - 2001
Externally publishedYes

Fingerprint

West Nile virus
Genes
Reverse Transcription
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Ebel, G. D., Dupuis, A. P., Ngo, K., Nicholas, D., Kauffman, E., Jones, S. A., ... Kramer, L. D. (2001). Partial genetic characterization of West Nile virus strains, New York State, 2000. Emerging Infectious Diseases, 7(4), 650-653.

Partial genetic characterization of West Nile virus strains, New York State, 2000. / Ebel, Gregory D.; Dupuis, Alan P.; Ngo, Kiet; Nicholas, David; Kauffman, Elizabeth; Jones, Susan A.; Young, Donna; Maffei, Joseph; Shi, Pei-Yong; Bernard, Kristen; Kramer, Laura D.

In: Emerging Infectious Diseases, Vol. 7, No. 4, 2001, p. 650-653.

Research output: Contribution to journalArticle

Ebel, GD, Dupuis, AP, Ngo, K, Nicholas, D, Kauffman, E, Jones, SA, Young, D, Maffei, J, Shi, P-Y, Bernard, K & Kramer, LD 2001, 'Partial genetic characterization of West Nile virus strains, New York State, 2000', Emerging Infectious Diseases, vol. 7, no. 4, pp. 650-653.
Ebel GD, Dupuis AP, Ngo K, Nicholas D, Kauffman E, Jones SA et al. Partial genetic characterization of West Nile virus strains, New York State, 2000. Emerging Infectious Diseases. 2001;7(4):650-653.
Ebel, Gregory D. ; Dupuis, Alan P. ; Ngo, Kiet ; Nicholas, David ; Kauffman, Elizabeth ; Jones, Susan A. ; Young, Donna ; Maffei, Joseph ; Shi, Pei-Yong ; Bernard, Kristen ; Kramer, Laura D. / Partial genetic characterization of West Nile virus strains, New York State, 2000. In: Emerging Infectious Diseases. 2001 ; Vol. 7, No. 4. pp. 650-653.
@article{aa56aa6b9e8840809577a842fa9711fa,
title = "Partial genetic characterization of West Nile virus strains, New York State, 2000",
abstract = "We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7{\%}) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.",
author = "Ebel, {Gregory D.} and Dupuis, {Alan P.} and Kiet Ngo and David Nicholas and Elizabeth Kauffman and Jones, {Susan A.} and Donna Young and Joseph Maffei and Pei-Yong Shi and Kristen Bernard and Kramer, {Laura D.}",
year = "2001",
language = "English (US)",
volume = "7",
pages = "650--653",
journal = "Emerging Infectious Diseases",
issn = "1080-6040",
publisher = "Centers for Disease Control and Prevention (CDC)",
number = "4",

}

TY - JOUR

T1 - Partial genetic characterization of West Nile virus strains, New York State, 2000

AU - Ebel, Gregory D.

AU - Dupuis, Alan P.

AU - Ngo, Kiet

AU - Nicholas, David

AU - Kauffman, Elizabeth

AU - Jones, Susan A.

AU - Young, Donna

AU - Maffei, Joseph

AU - Shi, Pei-Yong

AU - Bernard, Kristen

AU - Kramer, Laura D.

PY - 2001

Y1 - 2001

N2 - We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.

AB - We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.

UR - http://www.scopus.com/inward/record.url?scp=0034857547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034857547&partnerID=8YFLogxK

M3 - Article

C2 - 11585527

AN - SCOPUS:0034857547

VL - 7

SP - 650

EP - 653

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

SN - 1080-6040

IS - 4

ER -