Partial genetic characterization of West Nile virus strains, New York State, 2000

Gregory D. Ebel; Alan P. Dupuis; Kiet Ngo; David Nicholas; Elizabeth Kauffman; Susan A. Jones; Donna Young; Joseph Maffei; Pei Yong Shi; Kristen Bernard; Laura D. Kramer

doi:10.3201/eid0704.017408

Partial genetic characterization of West Nile virus strains, New York State, 2000

Gregory D. Ebel, Alan P. Dupuis, Kiet Ngo, David Nicholas, Elizabeth Kauffman, Susan A. Jones, Donna Young, Joseph Maffei, Pei Yong Shi, Kristen Bernard, Laura D. Kramer

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.

Original language	English (US)
Pages (from-to)	650-653
Number of pages	4
Journal	Emerging infectious diseases
Volume	7
Issue number	4
DOIs	https://doi.org/10.3201/eid0704.017408
State	Published - 2001

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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title = "Partial genetic characterization of West Nile virus strains, New York State, 2000",

abstract = "We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.",

author = "Ebel, {Gregory D.} and Dupuis, {Alan P.} and Kiet Ngo and David Nicholas and Elizabeth Kauffman and Jones, {Susan A.} and Donna Young and Joseph Maffei and Shi, {Pei Yong} and Kristen Bernard and Kramer, {Laura D.}",

year = "2001",

doi = "10.3201/eid0704.017408",

language = "English (US)",

volume = "7",

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journal = "Emerging infectious diseases",

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T1 - Partial genetic characterization of West Nile virus strains, New York State, 2000

AU - Ebel, Gregory D.

AU - Dupuis, Alan P.

AU - Ngo, Kiet

AU - Nicholas, David

AU - Kauffman, Elizabeth

AU - Jones, Susan A.

AU - Young, Donna

AU - Maffei, Joseph

AU - Shi, Pei Yong

AU - Bernard, Kristen

AU - Kramer, Laura D.

PY - 2001

Y1 - 2001

N2 - We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.

AB - We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.

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Partial genetic characterization of West Nile virus strains, New York State, 2000

Abstract

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