Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses

Charles M. Rice, Ruedi Aebersold, David B. Teplow, Janice Pata, John R. Bell, A. Vance Vorndam, Dennis W. Trent, Michael W. Brandriss, Jacob J. Schlesinger, James H. Strauss

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Abstract

Partial N-terminal amino acid sequences for the three largest nonstructural proteins of two flaviviruses, yellow fever virus and St. Louis encephalitis virus, have been obtained. The determined sequences of these proteins exhibit significant amino acid sequence homology, and allow the positioning of these three nonstructural proteins in the polyprotein sequence deduced from the nucleotide sequence of yellow fever virus (C. M. Rice, E. M. Lenches, S. R. Eddy, S. J. Shin, R. L. Sheets, and J. H. Strauss, 1985, Science229, 726-733.) The deduced start points support the hypothesis that the N terminus of nonstructural glycoprotein NS1 results from cleavage by signalase, whereas the N termini of NS3 and NS5 result from cleavages following double basic residues that are flanked by amino acids with short side chains.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalVirology
Volume151
Issue number1
DOIs
StatePublished - 1986

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Flavivirus
Yellow fever virus
Amino Acid Sequence
St. Louis Encephalitis Viruses
Polyproteins
Amino Acid Sequence Homology
Proteins
Glycoproteins
Amino Acids

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Rice, C. M., Aebersold, R., Teplow, D. B., Pata, J., Bell, J. R., Vance Vorndam, A., ... Strauss, J. H. (1986). Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses. Virology, 151(1), 1-9. https://doi.org/10.1016/0042-6822(86)90098-X

Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses. / Rice, Charles M.; Aebersold, Ruedi; Teplow, David B.; Pata, Janice; Bell, John R.; Vance Vorndam, A.; Trent, Dennis W.; Brandriss, Michael W.; Schlesinger, Jacob J.; Strauss, James H.

In: Virology, Vol. 151, No. 1, 1986, p. 1-9.

Research output: Contribution to journalArticle

Rice, CM, Aebersold, R, Teplow, DB, Pata, J, Bell, JR, Vance Vorndam, A, Trent, DW, Brandriss, MW, Schlesinger, JJ & Strauss, JH 1986, 'Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses', Virology, vol. 151, no. 1, pp. 1-9. https://doi.org/10.1016/0042-6822(86)90098-X
Rice, Charles M. ; Aebersold, Ruedi ; Teplow, David B. ; Pata, Janice ; Bell, John R. ; Vance Vorndam, A. ; Trent, Dennis W. ; Brandriss, Michael W. ; Schlesinger, Jacob J. ; Strauss, James H. / Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses. In: Virology. 1986 ; Vol. 151, No. 1. pp. 1-9.
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