TY - JOUR
T1 - Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses
AU - Rice, Charles M.
AU - Aebersold, Ruedi
AU - Teplow, David B.
AU - Pata, Janice
AU - Bell, John R.
AU - Vance Vorndam, A.
AU - Trent, Dennis W.
AU - Brandriss, Michael W.
AU - Schlesinger, Jacob J.
AU - Strauss, James H.
N1 - Funding Information:
We gratefully acknowledge the competent technical assistance of E. Lenches. We are grateful to E. Strauss for help with the manuscript and in producing the figures. The computer facility of L. Hood and the programs written by T. Hunkapiller were of great value in producing the figures. This work was supported by Grants AI 20612,A I 10793f rom NIH and Grant DMB 83-16856f rom NSF. R.A. was the recipient of a training fellowship from the Swiss National Foundation.
PY - 1986/5
Y1 - 1986/5
N2 - Partial N-terminal amino acid sequences for the three largest nonstructural proteins of two flaviviruses, yellow fever virus and St. Louis encephalitis virus, have been obtained. The determined sequences of these proteins exhibit significant amino acid sequence homology, and allow the positioning of these three nonstructural proteins in the polyprotein sequence deduced from the nucleotide sequence of yellow fever virus (C. M. Rice, E. M. Lenches, S. R. Eddy, S. J. Shin, R. L. Sheets, and J. H. Strauss, 1985, Science229, 726-733.) The deduced start points support the hypothesis that the N terminus of nonstructural glycoprotein NS1 results from cleavage by signalase, whereas the N termini of NS3 and NS5 result from cleavages following double basic residues that are flanked by amino acids with short side chains.
AB - Partial N-terminal amino acid sequences for the three largest nonstructural proteins of two flaviviruses, yellow fever virus and St. Louis encephalitis virus, have been obtained. The determined sequences of these proteins exhibit significant amino acid sequence homology, and allow the positioning of these three nonstructural proteins in the polyprotein sequence deduced from the nucleotide sequence of yellow fever virus (C. M. Rice, E. M. Lenches, S. R. Eddy, S. J. Shin, R. L. Sheets, and J. H. Strauss, 1985, Science229, 726-733.) The deduced start points support the hypothesis that the N terminus of nonstructural glycoprotein NS1 results from cleavage by signalase, whereas the N termini of NS3 and NS5 result from cleavages following double basic residues that are flanked by amino acids with short side chains.
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U2 - 10.1016/0042-6822(86)90098-X
DO - 10.1016/0042-6822(86)90098-X
M3 - Article
C2 - 3008425
AN - SCOPUS:0022600842
SN - 0042-6822
VL - 151
SP - 1
EP - 9
JO - Virology
JF - Virology
IS - 1
ER -