Partial nucleotide sequence of St. Louis encephalitis virus RNA

Structural proteins, NS1, ns2a, and ns2b

Dennis W. Trent, Richard M. Kinney, Barbara J B Johnson, A. Vance Vorndam, Joyce A. Grant, Vincent Deubel, Charles M. Rice, Chang Hahn

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

cDNA clones of the St. Louis encephalitis (SLE) virus genome have been obtained and the nucleotide sequence of 4.7 kb corresponding to the 5′ terminal half of the genome determined. The genome contains a 5′ noncoding region of 98 nucleotides followed by a single continuous open reading frame that encodes three structural proteins in the order capsid (C), membrane precursor (prM)-membrane (M), and envelope (E). Immediately following the C-terminus of E are located nonstructural proteins NS1 through NS3. The SLE amino acid sequence homology with yellow fever (YF), Murray Valley encephalitis (MVE), West Nile (WN), and dengue-2 (DEN) viruses over the sequenced region is 39, 66, 64, and 43%, respectively. The start of each SLE protein has been assigned on the basis of N-terminal sequence data and potential proteolytic cleavage sites homologous with YF and MVE viruses. Flaviviruses have conserved glycosylation sites in prM and NS1 proteins, although only one of the two glycosylation sites in the SLE E protein is conserved in MVE and DEN viruses. An evolutionary tree showing relationships of SLE, MVE, WN, YF, and DEN-2 flaviviruses is proposed on the basis of the amino acid sequences of the C proteins.

Original languageEnglish
Pages (from-to)293-304
Number of pages12
JournalVirology
Volume156
Issue number2
DOIs
StatePublished - 1987

Fingerprint

St. Louis Encephalitis Viruses
Viral Structural Proteins
St. Louis Encephalitis
Yellow Fever
RNA
Encephalitis
Flavivirus
Dengue Virus
Genome
Proteins
Glycosylation
Murray Valley encephalitis virus
West Nile Fever
Amino Acid Sequence Homology
Membranes
Dengue
Capsid
Protein C
Open Reading Frames
Amino Acid Sequence

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Trent, D. W., Kinney, R. M., Johnson, B. J. B., Vorndam, A. V., Grant, J. A., Deubel, V., ... Hahn, C. (1987). Partial nucleotide sequence of St. Louis encephalitis virus RNA: Structural proteins, NS1, ns2a, and ns2b. Virology, 156(2), 293-304. https://doi.org/10.1016/0042-6822(87)90409-0

Partial nucleotide sequence of St. Louis encephalitis virus RNA : Structural proteins, NS1, ns2a, and ns2b. / Trent, Dennis W.; Kinney, Richard M.; Johnson, Barbara J B; Vorndam, A. Vance; Grant, Joyce A.; Deubel, Vincent; Rice, Charles M.; Hahn, Chang.

In: Virology, Vol. 156, No. 2, 1987, p. 293-304.

Research output: Contribution to journalArticle

Trent, DW, Kinney, RM, Johnson, BJB, Vorndam, AV, Grant, JA, Deubel, V, Rice, CM & Hahn, C 1987, 'Partial nucleotide sequence of St. Louis encephalitis virus RNA: Structural proteins, NS1, ns2a, and ns2b', Virology, vol. 156, no. 2, pp. 293-304. https://doi.org/10.1016/0042-6822(87)90409-0
Trent, Dennis W. ; Kinney, Richard M. ; Johnson, Barbara J B ; Vorndam, A. Vance ; Grant, Joyce A. ; Deubel, Vincent ; Rice, Charles M. ; Hahn, Chang. / Partial nucleotide sequence of St. Louis encephalitis virus RNA : Structural proteins, NS1, ns2a, and ns2b. In: Virology. 1987 ; Vol. 156, No. 2. pp. 293-304.
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