Patch-clamp studies of voltagegated currents in identified neurons of the rat cerebral cortex

Owen Hamill, J. R. Huguenard, D. A. Prince

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

In the cerebral cortex, neurons can be classified into 2 broad morphological classes, referred to as pyramidal and nonpyramidal (stellate) cells, which correspond to functional classes of projection neurons and local circuit interneurons, respectively. In this study, we demonstrate that specific morphological, immunohistochemical, and physiological features, that allow class distinction of neurons in situ, are retained in acutely isolated neocortical neurons. Furthermore, voltage-clamp analysis with patch-clamp techniques indicate the differences in functional properties in adult neurons, reflect cell-specific, developmental changes in the density and type of specific classes of Na+, K+ and Ca2+ channels expressed. The differences in channel properties contribute to the different input-output relations of neocortical neurons, which enable inhibitory neurons to follow excitatory inputs faithfully and projection neurons to have more integrative roles.

Original languageEnglish (US)
Pages (from-to)48-61
Number of pages14
JournalCerebral Cortex
Volume1
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Patch Clamp
Clamping devices
Cortex
Cerebral Cortex
Neurons
Rats
Neuron
Projection
Rat
Cell
Patch-Clamp Techniques
Interneurons
Voltage

ASJC Scopus subject areas

  • Neuroscience(all)
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cognitive Neuroscience

Cite this

Patch-clamp studies of voltagegated currents in identified neurons of the rat cerebral cortex. / Hamill, Owen; Huguenard, J. R.; Prince, D. A.

In: Cerebral Cortex, Vol. 1, No. 1, 1991, p. 48-61.

Research output: Contribution to journalArticle

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