Pathoadaptive mutation that mediates adherence of Shiga toxin-producing Escherichia coli O111

Alfredo Torres, Roberta C. Vazquez-Juarez, Christopher B. Tutt, J. Gerardo Garcia-Gallegos

Research output: Contribution to journalArticle

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Abstract

The adherence of pathogenic Escherichia coli strains to intestinal epithelium is essential for initiation of infection. The cad operon encodes the lysine decarboxylase (LDC) system responsible for metabolizing lysine, and this operon has been proposed as an antivirulence mechanism in enteroinvasive E. coli and Shigella flexneri and as a factor mediating E. coli O157:H7 adherence. We sought to determine whether the LDC activity was present in a phylogenetically characterized collection of diarrheagenic E. coli (DEC) strains and to establish whether its expression was associated with their adherence to tissue culture cells. LDC activity was found in most of the pathogenic E. coli strains tested and was absent from Shiga toxin-producing E. coli (STEC) O111 strains (DEC pathotype 8). Analysis of the cad region in these O111 strains indicates that the operon has been rearranged and some of the genes are either missing or disrupted. A similar rearrangement was found in an E. coli O111:H8 strain recently isolated from an outbreak in Texas. Complementation of the LDC-negative strains with the cad operon in trans restored the LDC activity and resulted in a reduction in adherence to tissue culture cells. Initial analysis of the protein profiles on the surface of the O111 strains indicates that the LDC activity has an effect on the expression of the adhesin intimin. Cadaverine had a slight effect on LDC-negative strain adhesion but none on intimin expression. Our data suggest that this pathoadaptive mutation is an important mechanism to control functions potentially implicated in the pathogenesis of these organisms.

Original languageEnglish
Pages (from-to)4766-4776
Number of pages11
JournalInfection and Immunity
Volume73
Issue number8
DOIs
StatePublished - Aug 2005

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lysine decarboxylase
Shiga-Toxigenic Escherichia coli
Mutation
Operon
Escherichia coli
Cell Culture Techniques
Cadaverine
Shigella flexneri
Escherichia coli O157
Intestinal Mucosa
Lysine
Disease Outbreaks

ASJC Scopus subject areas

  • Immunology

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Pathoadaptive mutation that mediates adherence of Shiga toxin-producing Escherichia coli O111. / Torres, Alfredo; Vazquez-Juarez, Roberta C.; Tutt, Christopher B.; Gerardo Garcia-Gallegos, J.

In: Infection and Immunity, Vol. 73, No. 8, 08.2005, p. 4766-4776.

Research output: Contribution to journalArticle

Torres, Alfredo ; Vazquez-Juarez, Roberta C. ; Tutt, Christopher B. ; Gerardo Garcia-Gallegos, J. / Pathoadaptive mutation that mediates adherence of Shiga toxin-producing Escherichia coli O111. In: Infection and Immunity. 2005 ; Vol. 73, No. 8. pp. 4766-4776.
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