Pathogen and host response dynamics in a mouse model of Borrelia hermsii relapsing fever

Christopher D. Crowder, Arash Ghalyanchi Langeroudi, Azadeh Shojaee Estabragh, Eric R.G. Lewis, Renee A. Marcsisin, Alan G. Barbour

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Most Borrelia species that cause tick-borne relapsing fever utilize rodents as their natural reservoirs, and for decades laboratory-bred rodents have served as informative experimental models for the disease. However, while there has much progress in understanding the pathogenetic mechanisms, including antigenic variation, of the pathogen, the host side of the equation has been neglected. Using different approaches, we studied, in immunocompetent inbred mice, the dynamics of infection with and host responses to North American relapsing fever agent B. hermsii. The spirochete's generation time in blood of infected mice was between 4-5 h and, after a delay, was matched in rate by the increase of specific agglutinating antibodies in response to the infection. After initiating serotype cells were cleared by antibodies, the surviving spirochetes were a different serotype and, as a population, grew more slowly. The retardation was attributable to the host response and not an inherently slower growth rate. The innate responses at infection peak and immediate aftermath were characterized by elevations of both pro-inflammatory and anti-inflammatory cytokines and chemokines. Immunodeficient mice had higher spirochete burdens and severe anemia, which was accounted for by aggregation of erythrocytes by spirochetes and their partially reversible sequestration in greatly enlarged spleens and elsewhere.

Original languageEnglish (US)
Article number19
JournalVeterinary Sciences
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Fingerprint

Borrelia hermsii
Relapsing Fever
Borrelia
Spirochaetales
dynamic models
fever
animal models
pathogens
Rodentia
mice
serotypes
rodents
Infection
Ornithodoros
infection
Erythrocyte Aggregation
Antigenic Variation
antigenic variation
antibodies
Splenomegaly

Keywords

  • Antigenic variation
  • Mus musculus
  • Ornithodoros
  • Spirochete
  • Tick-borne disease
  • Zoonosis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Crowder, C. D., Langeroudi, A. G., Estabragh, A. S., Lewis, E. R. G., Marcsisin, R. A., & Barbour, A. G. (2016). Pathogen and host response dynamics in a mouse model of Borrelia hermsii relapsing fever. Veterinary Sciences, 3(3), [19]. https://doi.org/10.3390/vetsci3030019

Pathogen and host response dynamics in a mouse model of Borrelia hermsii relapsing fever. / Crowder, Christopher D.; Langeroudi, Arash Ghalyanchi; Estabragh, Azadeh Shojaee; Lewis, Eric R.G.; Marcsisin, Renee A.; Barbour, Alan G.

In: Veterinary Sciences, Vol. 3, No. 3, 19, 01.09.2016.

Research output: Contribution to journalArticle

Crowder, CD, Langeroudi, AG, Estabragh, AS, Lewis, ERG, Marcsisin, RA & Barbour, AG 2016, 'Pathogen and host response dynamics in a mouse model of Borrelia hermsii relapsing fever', Veterinary Sciences, vol. 3, no. 3, 19. https://doi.org/10.3390/vetsci3030019
Crowder CD, Langeroudi AG, Estabragh AS, Lewis ERG, Marcsisin RA, Barbour AG. Pathogen and host response dynamics in a mouse model of Borrelia hermsii relapsing fever. Veterinary Sciences. 2016 Sep 1;3(3). 19. https://doi.org/10.3390/vetsci3030019
Crowder, Christopher D. ; Langeroudi, Arash Ghalyanchi ; Estabragh, Azadeh Shojaee ; Lewis, Eric R.G. ; Marcsisin, Renee A. ; Barbour, Alan G. / Pathogen and host response dynamics in a mouse model of Borrelia hermsii relapsing fever. In: Veterinary Sciences. 2016 ; Vol. 3, No. 3.
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