Pathogenesis of lassa fever in cynomolgus macaques

Lisa E. Hensley, Mark A. Smith, Joan B. Geisbert, Elizabeth A. Fritz, Kathleen M. Daddario-Dicaprio, Tom Larsen, Thomas Geisbert

Research output: Contribution to journalArticle

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Abstract

Background: Lassa virus (LASV) infection causes an acute and sometimes fatal hemorrhagic disease in humans and nonhuman primates; however, little is known about the development of Lassa fever. Here, we performed a pilot study to begin to understand the progression of LASV infection in nonhuman primates. Methods. Six cynomolgus monkeys were experimentally infected with LASV. Tissues from three animals were examined at an early- to mid-stage of disease and compared with tissues from three animals collected at terminal stages of disease. Results: Dendritic cells were identified as a prominent target of LASV infection in a variety of tissues in all animals at day 7 while Kupffer cells, hepatocytes, adrenal cortical cells, and endothelial cells were more frequently infected with LASV in tissues of terminal animals (days 13.5-17). Meningoencephalitis and neuronal necrosis were noteworthy findings in terminal animals. Evidence of coagulopathy was noted; however, the degree of fibrin deposition in tissues was less prominent than has been reported in other viral hemorrhagic fevers. Conclusion: The sequence of pathogenic events identified in this study begins to shed light on the development of disease processes during Lassa fever and also may provide new targets for rational prophylactic and chemotherapeutic interventions.

Original languageEnglish (US)
Article number205
JournalVirology Journal
Volume8
DOIs
StatePublished - 2011

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Lassa Fever
Lassa virus
Macaca
Virus Diseases
Primates
Viral Hemorrhagic Fevers
Meningoencephalitis
Kupffer Cells
Macaca fascicularis
Fibrin
Dendritic Cells
Hepatocytes
Necrosis
Endothelial Cells

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Hensley, L. E., Smith, M. A., Geisbert, J. B., Fritz, E. A., Daddario-Dicaprio, K. M., Larsen, T., & Geisbert, T. (2011). Pathogenesis of lassa fever in cynomolgus macaques. Virology Journal, 8, [205]. https://doi.org/10.1186/1743-422X-8-205

Pathogenesis of lassa fever in cynomolgus macaques. / Hensley, Lisa E.; Smith, Mark A.; Geisbert, Joan B.; Fritz, Elizabeth A.; Daddario-Dicaprio, Kathleen M.; Larsen, Tom; Geisbert, Thomas.

In: Virology Journal, Vol. 8, 205, 2011.

Research output: Contribution to journalArticle

Hensley, LE, Smith, MA, Geisbert, JB, Fritz, EA, Daddario-Dicaprio, KM, Larsen, T & Geisbert, T 2011, 'Pathogenesis of lassa fever in cynomolgus macaques', Virology Journal, vol. 8, 205. https://doi.org/10.1186/1743-422X-8-205
Hensley LE, Smith MA, Geisbert JB, Fritz EA, Daddario-Dicaprio KM, Larsen T et al. Pathogenesis of lassa fever in cynomolgus macaques. Virology Journal. 2011;8. 205. https://doi.org/10.1186/1743-422X-8-205
Hensley, Lisa E. ; Smith, Mark A. ; Geisbert, Joan B. ; Fritz, Elizabeth A. ; Daddario-Dicaprio, Kathleen M. ; Larsen, Tom ; Geisbert, Thomas. / Pathogenesis of lassa fever in cynomolgus macaques. In: Virology Journal. 2011 ; Vol. 8.
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