Pathogenesis of XJ and Romero strains of Junin virus in two strains of guinea pigs

Nadezhda E. Yun, Nathaniel S. Linde, Natallia Dziuba, Michele A. Zacks, Jeanon N. Smith, Jennifer K. Smith, Judith Aronson, Olga V. Chumakova, Heather M. Lander, Clarence J. Peters, Slobodan Paessler

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Argentine hemorrhagic fever (AHF), a systemic infectious disease caused by infection with Junin virus, affects several organs, and patients can show hematologic, cardiovascular, renal, or neurologic symptoms. We compared the virulence of two Junin virus strains in inbred and outbred guinea pigs with the aim of characterizing this animal model better for future vaccine/antiviral efficacy studies. Our data indicate that this passage of the XJ strain is attenuated in guinea pigs. In contrast, the Romero strain is highly virulent in Strain 13 as well as in Hartley guinea pigs, resulting in systemic infection, thrombocytopenia, elevated apartate aminotransferase levels, and ultimately, uniformly lethal disease. We detected viral antigen in formalin-fixed, paraffin-embedded tissues. Thus, both guinea pig strains are useful animal models for lethal Junin virus (Romero strain) infection and potentially can be used for preclinical trials in vaccine or antiviral drug development.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume79
Issue number2
StatePublished - Aug 2008

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Junin virus
Guinea Pigs
Antiviral Agents
Vaccines
Animal Models
Infection
Viral Antigens
Neurologic Manifestations
Transaminases
Thrombocytopenia
Paraffin
Formaldehyde
Communicable Diseases
Virulence
Fever
Kidney

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Yun, N. E., Linde, N. S., Dziuba, N., Zacks, M. A., Smith, J. N., Smith, J. K., ... Paessler, S. (2008). Pathogenesis of XJ and Romero strains of Junin virus in two strains of guinea pigs. American Journal of Tropical Medicine and Hygiene, 79(2), 275-282.

Pathogenesis of XJ and Romero strains of Junin virus in two strains of guinea pigs. / Yun, Nadezhda E.; Linde, Nathaniel S.; Dziuba, Natallia; Zacks, Michele A.; Smith, Jeanon N.; Smith, Jennifer K.; Aronson, Judith; Chumakova, Olga V.; Lander, Heather M.; Peters, Clarence J.; Paessler, Slobodan.

In: American Journal of Tropical Medicine and Hygiene, Vol. 79, No. 2, 08.2008, p. 275-282.

Research output: Contribution to journalArticle

Yun, NE, Linde, NS, Dziuba, N, Zacks, MA, Smith, JN, Smith, JK, Aronson, J, Chumakova, OV, Lander, HM, Peters, CJ & Paessler, S 2008, 'Pathogenesis of XJ and Romero strains of Junin virus in two strains of guinea pigs', American Journal of Tropical Medicine and Hygiene, vol. 79, no. 2, pp. 275-282.
Yun NE, Linde NS, Dziuba N, Zacks MA, Smith JN, Smith JK et al. Pathogenesis of XJ and Romero strains of Junin virus in two strains of guinea pigs. American Journal of Tropical Medicine and Hygiene. 2008 Aug;79(2):275-282.
Yun, Nadezhda E. ; Linde, Nathaniel S. ; Dziuba, Natallia ; Zacks, Michele A. ; Smith, Jeanon N. ; Smith, Jennifer K. ; Aronson, Judith ; Chumakova, Olga V. ; Lander, Heather M. ; Peters, Clarence J. ; Paessler, Slobodan. / Pathogenesis of XJ and Romero strains of Junin virus in two strains of guinea pigs. In: American Journal of Tropical Medicine and Hygiene. 2008 ; Vol. 79, No. 2. pp. 275-282.
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