Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes.

J. C. Morrill, G. B. Jennings, H. Caplen, M. J. Turell, A. J. Johnson, C. J. Peters

Research output: Contribution to journalArticle

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Abstract

A mutagenized clone of Rift Valley fever virus (RVFV; MV P12) used in inoculation of 3 pregnant ewes was immunogenic, nonpathogenic, and nonabortogenic. In contrast, inoculation of a matched group of 3 pregnant ewes with parent RVFV induced clinical disease and abortions. Ewes given MV P12 delivered healthy lambs that had RVFV antibody titers of less than 1:10 at birth, increasing to greater than or equal to 1:80 after ingestion of colostrum. Ewes inoculated with parent RVFV developed marked viremia, followed by RVFV antibody titers greater than or equal to 1:1,280; ewes inoculated with MV P12 developed low viremia titers and RVFV antibody titers of 1:80 to 1:320. Postpartum challenge exposure of the previously MV P12-inoculated ewes with virulent Zagazig human 501 strain RVFV indicated that the ewes were protected from clinical disease. The RVFV-susceptible female Culex pipiens that fed on the MV P12-inoculated ewes failed to transmit RVFV to hamsters; mosquitoes that fed on the parent RVFV-inoculated ewes became infected and transmitted RVFV to hamsters.

Original languageEnglish (US)
Pages (from-to)1042-1047
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume48
Issue number7
StatePublished - Jul 1987
Externally publishedYes

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Rift Valley fever virus
Mutagens
Virulence
ewes
pathogenicity
Viremia
immune response
antigens
Cricetinae
Antibodies
Culex
Colostrum
Culicidae
Postpartum Period
Research Design
Clone Cells
Eating
viremia
Parturition
hamsters

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Morrill, J. C., Jennings, G. B., Caplen, H., Turell, M. J., Johnson, A. J., & Peters, C. J. (1987). Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes. American Journal of Veterinary Research, 48(7), 1042-1047.

Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes. / Morrill, J. C.; Jennings, G. B.; Caplen, H.; Turell, M. J.; Johnson, A. J.; Peters, C. J.

In: American Journal of Veterinary Research, Vol. 48, No. 7, 07.1987, p. 1042-1047.

Research output: Contribution to journalArticle

Morrill, JC, Jennings, GB, Caplen, H, Turell, MJ, Johnson, AJ & Peters, CJ 1987, 'Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes.', American Journal of Veterinary Research, vol. 48, no. 7, pp. 1042-1047.
Morrill, J. C. ; Jennings, G. B. ; Caplen, H. ; Turell, M. J. ; Johnson, A. J. ; Peters, C. J. / Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes. In: American Journal of Veterinary Research. 1987 ; Vol. 48, No. 7. pp. 1042-1047.
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