Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes.

J. C. Morrill, G. B. Jennings, H. Caplen, M. J. Turell, A. J. Johnson, C. J. Peters

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

A mutagenized clone of Rift Valley fever virus (RVFV; MV P12) used in inoculation of 3 pregnant ewes was immunogenic, nonpathogenic, and nonabortogenic. In contrast, inoculation of a matched group of 3 pregnant ewes with parent RVFV induced clinical disease and abortions. Ewes given MV P12 delivered healthy lambs that had RVFV antibody titers of less than 1:10 at birth, increasing to greater than or equal to 1:80 after ingestion of colostrum. Ewes inoculated with parent RVFV developed marked viremia, followed by RVFV antibody titers greater than or equal to 1:1,280; ewes inoculated with MV P12 developed low viremia titers and RVFV antibody titers of 1:80 to 1:320. Postpartum challenge exposure of the previously MV P12-inoculated ewes with virulent Zagazig human 501 strain RVFV indicated that the ewes were protected from clinical disease. The RVFV-susceptible female Culex pipiens that fed on the MV P12-inoculated ewes failed to transmit RVFV to hamsters; mosquitoes that fed on the parent RVFV-inoculated ewes became infected and transmitted RVFV to hamsters.

Original languageEnglish (US)
Pages (from-to)1042-1047
Number of pages6
JournalAmerican journal of veterinary research
Volume48
Issue number7
StatePublished - Jul 1 1987

ASJC Scopus subject areas

  • veterinary(all)

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    Morrill, J. C., Jennings, G. B., Caplen, H., Turell, M. J., Johnson, A. J., & Peters, C. J. (1987). Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes. American journal of veterinary research, 48(7), 1042-1047.