Pathogenicity Comparison between the Kikwit and Makona Ebola Virus Variants in Rhesus Macaques

Gary Wong, Xiangguo Qiu, Marc Antoine De La Vega, Lisa Fernando, Haiyan Wei, Alexander Bello, Hugues Fausther-Bovendo, Jonathan Audet, Andrea Kroeker, Robert Kozak, Kaylie Tran, Shihua He, Kevin Tierney, Geoff Soule, Estella Moffat, Stephan Günther, George F. Gao, Jim Strong, Carissa Embury-Hyatt, Gary Kobinger

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Enhanced virulence and/or transmission of West African Ebola virus (EBOV) variants, which are divergent from their Central African counterparts, are suspected to have contributed to the sizable toll of the recent Ebola virus disease (EVD) outbreak. This study evaluated the pathogenicity and shedding in rhesus macaques infected with 1 of 2 West African isolates (EBOV-C05 or EBOV-C07) or a Central African isolate (EBOV-K). All animals infected with EBOV-C05 or EBOV-C07 died of EVD, whereas 2 of 3 EBOV-K-infected animals died. The viremia level was elevated 10-fold in EBOV-C05-infected animals, compared with EBOV-C07- or EBOV-K-infected animals. More-severe lung pathology was observed in 2 of 6 EBOV-C05/C07-infected macaques. This is the first detailed analysis of the recently circulating EBOV-C05/C07 in direct comparison to EBOV-K with 6 animals per group, and it showed that EBOV-C05 but not EBOV-C07 can replicate at higher levels and cause more tissue damage in some animals. Increased virus shedding from individuals who are especially susceptible to EBOV replication is possibly one of the many challenges facing the community of healthcare and policy-making responders since the beginning of the outbreak.

Original languageEnglish (US)
Pages (from-to)S281-S289
JournalJournal of Infectious Diseases
Volume214
DOIs
StatePublished - Oct 15 2016
Externally publishedYes

Keywords

  • Ebola virus
  • Kikwit
  • Makona
  • Nonhuman primates
  • Pathogenicity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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