Pathology of fatal west nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York

K. E. Steele, M. J. Linn, R. J. Schoepp, N. Komar, Thomas Geisbert, R. M. Manduca, P. P. Calle, B. L. Raphael, T. L. Clippinger, T. Larsen, J. Smith, R. S. Lanciotti, N. A. Panella, T. S. McNamara

Research output: Contribution to journalArticle

330 Citations (Scopus)

Abstract

West Nile fever caused fatal disease in humans, horses, and birds in the northeastern United States during 1999. We studied birds from two wildlife facilities in New York City, New York, that died or were euthanatized and were suspected to have West Nile virus infections. Using standard histologic and ultrastructural methods, virus isolation, immunohistochemistry, in situ hybridization and reverse-transcriptase polymerase chain reaction, we identified West Nile virus as the cause of clinical disease, severe pathologic changes, and death in 27 birds representing eight orders and 14 species. Virus was detected in 23/26 brains (88%), 24/ 25 hearts (96%), 15/18 spleens (83%), 14/20 livers (70%), 20/20 kidneys (100%), 10/13 adrenals (77%), 13/ 14 intestines (93%), 10/12 pancreata (83%), 5/12 lungs (42%), and 4/8 ovaries (50%) by one or more methods. Cellular targets included neurons and glial cells in the brain, spinal cord, and peripheral ganglia; myocardial fibers; macrophages and blood monocytes; renal tubular epithelium; adrenal cortical cells; pancreatic acinar cells and islet cells; intestinal crypt epithelium; oocytes; and fibroblasts and smooth muscle cells. Purkinje cells were especially targeted, except in crows and magpies. Gross hemorrhage of the brain, splenomegaly, meningoencephalitis, and myocarditis were the most prominent lesions. Immunohistochemistry was an efficient and reliable method for identifying infected cases, but the polyclonal antibody cross-reacted with St. Louis encephalitis virus and other flaviviruses. In contrast, the in situ hybridization probe pWNV-E (WN-USAMRIID99) reacted only with West Nile virus. These methods should aid diagnosticians faced with the emergence of West Nile virus in the United States.

Original languageEnglish (US)
Pages (from-to)208-224
Number of pages17
JournalVeterinary Pathology
Volume37
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

West Nile virus
Virus Diseases
Birds
Disease Outbreaks
Pathology
birds
brain
infection
in situ hybridization
In Situ Hybridization
immunohistochemistry
West Nile Fever
St. Louis Encephalitis Viruses
epithelium
Immunohistochemistry
Saint Louis encephalitis virus
kidneys
Flaviviridae
intestinal crypts
Viruses

Keywords

  • Arbovirus
  • Avian
  • Immunohistochemistry
  • In situ hybridization
  • Meningoencephalitis
  • Myocarditis
  • RT-PCR
  • Virus isolation
  • West nile virus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Steele, K. E., Linn, M. J., Schoepp, R. J., Komar, N., Geisbert, T., Manduca, R. M., ... McNamara, T. S. (2000). Pathology of fatal west nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York. Veterinary Pathology, 37(3), 208-224.

Pathology of fatal west nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York. / Steele, K. E.; Linn, M. J.; Schoepp, R. J.; Komar, N.; Geisbert, Thomas; Manduca, R. M.; Calle, P. P.; Raphael, B. L.; Clippinger, T. L.; Larsen, T.; Smith, J.; Lanciotti, R. S.; Panella, N. A.; McNamara, T. S.

In: Veterinary Pathology, Vol. 37, No. 3, 2000, p. 208-224.

Research output: Contribution to journalArticle

Steele, KE, Linn, MJ, Schoepp, RJ, Komar, N, Geisbert, T, Manduca, RM, Calle, PP, Raphael, BL, Clippinger, TL, Larsen, T, Smith, J, Lanciotti, RS, Panella, NA & McNamara, TS 2000, 'Pathology of fatal west nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York', Veterinary Pathology, vol. 37, no. 3, pp. 208-224.
Steele, K. E. ; Linn, M. J. ; Schoepp, R. J. ; Komar, N. ; Geisbert, Thomas ; Manduca, R. M. ; Calle, P. P. ; Raphael, B. L. ; Clippinger, T. L. ; Larsen, T. ; Smith, J. ; Lanciotti, R. S. ; Panella, N. A. ; McNamara, T. S. / Pathology of fatal west nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York. In: Veterinary Pathology. 2000 ; Vol. 37, No. 3. pp. 208-224.
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