Patterns of eastern equine encephalomyelitis virus infection in Culiseta melanura (Diptera: Culicidae).

Scott Weaver, T. W. Scott, L. H. Lorenz

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Infection of the mosquito Culiseta melanura (Coquillett) by eastern equine encephalomyelitis virus was examined using transmission electron microscopy (TEM) of whole tagmata and fluorescent antibody assay (FA) and infectious assay (IA) of dissected tissues. Following infectious blood meals from chicks circulating different virus titers, mosquitoes were examined after extrinsic incubation intervals of 1-22 d. Virus was first detected by FA and IA in midguts and nonalimentary tissues 24 h after infection. Nascent virus was first visualized by TEM in several tissues, including midgut, fat body, and salivary glands, of high-titer-infected mosquitoes 48 h after they engorged. Moderate- and low-titer blood meals resulted in slightly slower appearance and dissemination of virus. Results were consistent with dissemination of virus from the posterior midgut to salivary glands via the hemolymph. Neural tissues contained little or no virus, whereas fat body appeared to be an important organ for virus replication and dissemination. Dissemination barriers did not accompany mosquito infections.

Original languageEnglish (US)
Pages (from-to)878-891
Number of pages14
JournalJournal of Medical Entomology
Volume27
Issue number5
StatePublished - Sep 1990
Externally publishedYes

Fingerprint

Equine Encephalomyelitis
Eastern equine encephalitis virus
Culiseta melanura
Culicidae
Diptera
Viruses
viruses
midgut
Fat Body
infection
blood meal
assays
fat body
salivary glands
Salivary Glands
Transmission Electron Microscopy
transmission electron microscopy
Meals
Infection
antibodies

ASJC Scopus subject areas

  • Insect Science

Cite this

Patterns of eastern equine encephalomyelitis virus infection in Culiseta melanura (Diptera : Culicidae). / Weaver, Scott; Scott, T. W.; Lorenz, L. H.

In: Journal of Medical Entomology, Vol. 27, No. 5, 09.1990, p. 878-891.

Research output: Contribution to journalArticle

@article{eb2146ab93b7402abd8b69e25901eb55,
title = "Patterns of eastern equine encephalomyelitis virus infection in Culiseta melanura (Diptera: Culicidae).",
abstract = "Infection of the mosquito Culiseta melanura (Coquillett) by eastern equine encephalomyelitis virus was examined using transmission electron microscopy (TEM) of whole tagmata and fluorescent antibody assay (FA) and infectious assay (IA) of dissected tissues. Following infectious blood meals from chicks circulating different virus titers, mosquitoes were examined after extrinsic incubation intervals of 1-22 d. Virus was first detected by FA and IA in midguts and nonalimentary tissues 24 h after infection. Nascent virus was first visualized by TEM in several tissues, including midgut, fat body, and salivary glands, of high-titer-infected mosquitoes 48 h after they engorged. Moderate- and low-titer blood meals resulted in slightly slower appearance and dissemination of virus. Results were consistent with dissemination of virus from the posterior midgut to salivary glands via the hemolymph. Neural tissues contained little or no virus, whereas fat body appeared to be an important organ for virus replication and dissemination. Dissemination barriers did not accompany mosquito infections.",
author = "Scott Weaver and Scott, {T. W.} and Lorenz, {L. H.}",
year = "1990",
month = "9",
language = "English (US)",
volume = "27",
pages = "878--891",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "5",

}

TY - JOUR

T1 - Patterns of eastern equine encephalomyelitis virus infection in Culiseta melanura (Diptera

T2 - Culicidae).

AU - Weaver, Scott

AU - Scott, T. W.

AU - Lorenz, L. H.

PY - 1990/9

Y1 - 1990/9

N2 - Infection of the mosquito Culiseta melanura (Coquillett) by eastern equine encephalomyelitis virus was examined using transmission electron microscopy (TEM) of whole tagmata and fluorescent antibody assay (FA) and infectious assay (IA) of dissected tissues. Following infectious blood meals from chicks circulating different virus titers, mosquitoes were examined after extrinsic incubation intervals of 1-22 d. Virus was first detected by FA and IA in midguts and nonalimentary tissues 24 h after infection. Nascent virus was first visualized by TEM in several tissues, including midgut, fat body, and salivary glands, of high-titer-infected mosquitoes 48 h after they engorged. Moderate- and low-titer blood meals resulted in slightly slower appearance and dissemination of virus. Results were consistent with dissemination of virus from the posterior midgut to salivary glands via the hemolymph. Neural tissues contained little or no virus, whereas fat body appeared to be an important organ for virus replication and dissemination. Dissemination barriers did not accompany mosquito infections.

AB - Infection of the mosquito Culiseta melanura (Coquillett) by eastern equine encephalomyelitis virus was examined using transmission electron microscopy (TEM) of whole tagmata and fluorescent antibody assay (FA) and infectious assay (IA) of dissected tissues. Following infectious blood meals from chicks circulating different virus titers, mosquitoes were examined after extrinsic incubation intervals of 1-22 d. Virus was first detected by FA and IA in midguts and nonalimentary tissues 24 h after infection. Nascent virus was first visualized by TEM in several tissues, including midgut, fat body, and salivary glands, of high-titer-infected mosquitoes 48 h after they engorged. Moderate- and low-titer blood meals resulted in slightly slower appearance and dissemination of virus. Results were consistent with dissemination of virus from the posterior midgut to salivary glands via the hemolymph. Neural tissues contained little or no virus, whereas fat body appeared to be an important organ for virus replication and dissemination. Dissemination barriers did not accompany mosquito infections.

UR - http://www.scopus.com/inward/record.url?scp=0025485419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025485419&partnerID=8YFLogxK

M3 - Article

C2 - 1977915

AN - SCOPUS:0025485419

VL - 27

SP - 878

EP - 891

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 5

ER -