TY - JOUR
T1 - Pelagia noctiluca (Scyphozoa) crude venom injection elicits oxidative stress and inflammatory response in rats
AU - Bruschetta, Giuseppe
AU - Impellizzeri, Daniela
AU - Morabito, Rossana
AU - Marino, Angela
AU - Ahmad, Akbar
AU - Spanò, Nunziacarla
AU - La Spada, Giuseppa
AU - Cuzzocrea, Salvatore
AU - Esposito, Emanuela
PY - 2014/4
Y1 - 2014/4
N2 - Cnidarian toxins represent a rich source of biologically active compounds. Since they may act via oxidative stress events, the aim of the present study was to verify whether crude venom, extracted from the jellyfish Pelagia noctiluca, elicits inflammation and oxidative stress processes, known to be mediated by Reactive Oxygen Species (ROS) production, in rats. In a first set of experiments, the animals were injected with crude venom (at three different doses 6, 30 and 60 μg/kg, suspended in saline solution, i.v.) to test the mortality and possible blood pressure changes. In a second set of experiments, to confirm that Pelagia noctiluca crude venom enhances ROS formation and may contribute to the pathophysiology of inflammation, crude venom-injected animals (30 μg/kg) were also treated with tempol, a powerful antioxidant (100 mg/kg i.p., 30 and 60 min after crude venom). Administration of tempol after crude venom challenge, caused a significant reduction of each parameter related to inflammation. The potential effect of Pelagia noctiluca crude venom in the systemic inflammation process has been here demonstrated, adding novel information about its biological activity.
AB - Cnidarian toxins represent a rich source of biologically active compounds. Since they may act via oxidative stress events, the aim of the present study was to verify whether crude venom, extracted from the jellyfish Pelagia noctiluca, elicits inflammation and oxidative stress processes, known to be mediated by Reactive Oxygen Species (ROS) production, in rats. In a first set of experiments, the animals were injected with crude venom (at three different doses 6, 30 and 60 μg/kg, suspended in saline solution, i.v.) to test the mortality and possible blood pressure changes. In a second set of experiments, to confirm that Pelagia noctiluca crude venom enhances ROS formation and may contribute to the pathophysiology of inflammation, crude venom-injected animals (30 μg/kg) were also treated with tempol, a powerful antioxidant (100 mg/kg i.p., 30 and 60 min after crude venom). Administration of tempol after crude venom challenge, caused a significant reduction of each parameter related to inflammation. The potential effect of Pelagia noctiluca crude venom in the systemic inflammation process has been here demonstrated, adding novel information about its biological activity.
KW - Apoptosis
KW - Crude venom
KW - Inflammation
KW - Oxidative stress
KW - Pelagia noctiluca
KW - Tempol
UR - http://www.scopus.com/inward/record.url?scp=84900404932&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84900404932&partnerID=8YFLogxK
U2 - 10.3390/md12042182
DO - 10.3390/md12042182
M3 - Article
C2 - 24727391
AN - SCOPUS:84900404932
VL - 12
SP - 2182
EP - 2204
JO - Marine Drugs
JF - Marine Drugs
SN - 1660-3397
IS - 4
ER -