Peptide conformations for a microarray surface-tethered epitope of the tumor suppressor p53

Jun Feng, Ka Yiu Wong, Gillian C. Lynch, Xiaolian Gao, Bernard Pettitt

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Peptides or proteins near surfaces exhibit different structural properties from those present in a homogeneous solution, and these differences give rise to varied biological activity. Therefore, understanding the detailed molecular structure of these molecules tethered to a surface is important for interpreting the performance of the various microarrays based on the activities of the immobilized peptides or proteins. We performed molecular dynamics simulations of a pentapeptide, RHSVV, an epitope of the tumor suppressor protein p53, tethered via a spacer on a functionalized silica surface and free in solution, to study their structural and conformational differences. These calculations allowed analyses of the peptide-surface interactions, the sequence orientations, and the translational motions of the peptide on the surface to be performed. Conformational similarities are found among dominant structures of the tethered and free peptide. In the peptide microarray simulations, the peptide fluctuates between a parallel and tilted orientation driven in part by the hydrophobic interactions between the nonpolar peptide residues and the methyl-terminated silica surface. The perpendicular movement of the peptide relative to the surface is also restricted due to the hydrophobic nature of the microarray surface. With regard to structures available for recognition and binding, we find that similar conformations to those found in solution are available to the peptide tethered to the surface, but with a shifted equilibrium constant. Comparisons with experimental results show important implications of this for peptide microarray design and assays.

Original languageEnglish (US)
Pages (from-to)13797-13806
Number of pages10
JournalJournal of Physical Chemistry B
Volume111
Issue number49
DOIs
StatePublished - Dec 13 2007
Externally publishedYes

Fingerprint

Epitopes
suppressors
Microarrays
Peptides
peptides
Conformations
Tumors
tumors
Proteins
Silicon Dioxide
tumor suppressor proteins
Silica
silicon dioxide
proteins
Tumor Suppressor Protein p53
translational motion
Equilibrium constants
activity (biology)
Bioactivity
spacers

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Peptide conformations for a microarray surface-tethered epitope of the tumor suppressor p53. / Feng, Jun; Wong, Ka Yiu; Lynch, Gillian C.; Gao, Xiaolian; Pettitt, Bernard.

In: Journal of Physical Chemistry B, Vol. 111, No. 49, 13.12.2007, p. 13797-13806.

Research output: Contribution to journalArticle

Feng, Jun ; Wong, Ka Yiu ; Lynch, Gillian C. ; Gao, Xiaolian ; Pettitt, Bernard. / Peptide conformations for a microarray surface-tethered epitope of the tumor suppressor p53. In: Journal of Physical Chemistry B. 2007 ; Vol. 111, No. 49. pp. 13797-13806.
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