Peptide nanofiber-CaCO3 composite microparticles as adjuvant-free oral vaccine delivery vehicles

Joshua D. Snook, Charles B. Chesson, Alex-Giovanny Peniche-Trujillo, Sara Dann-Grice, Adriana Paulucci, Iryna Pinchuk, Jai S. Rudra

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To combat mucosal pathogens that cause gastrointestinal (GI) infections, local mucosal immunity is required which is best achieved through oral vaccination. Oral delivery of vaccines is also a safe and convenient alternative to injected vaccines due to its non-invasive nature and high compliance rate for all ages. However, the lack of effective and safe mucosal adjuvants, the selective permeability of the mucus barrier, and the harsh GI environment continue to pose a significant challenge for oral vaccine development. Microparticle-based strategies are attractive for oral vaccination due to their ability to efficiently penetrate the mucus barrier and have the added advantage of protecting the antigen in the harsh gastric environment. In this work, self-adjuvanting peptide nanofiber-CaCO3 composite microparticles were prepared and investigated for oral vaccine delivery. Compared to polymeric microparticles, inorganic CaCO3 microparticles have unique advantages due to the biocompatibility of CaCO3 as a natural mineral, mild preparation conditions, and its porous structure that is suitable for loading other materials. Particle size distribution, nanofiber loading efficiency, morphology, and degradation in simulated gastric fluid were characterized. The composite microparticles were efficient at penetrating the mucus barrier and were localized to immune inductive sites and elicited the production of mucosal antibody responses, particularly the protective IgA isotype following oral administration. The magnitude of the mucosal immune response was comparable to the gold-standard adjuvant cholera toxin B (CTB). Our results indicate that OVA-KFE8/CaCO3 composite microparticles are efficient self-adjuvanting oral vaccine delivery vehicles for induction of mucosal antibody responses.

Original languageEnglish (US)
Pages (from-to)1640-1649
Number of pages10
JournalJournal of Materials Chemistry B
Volume4
Issue number9
DOIs
StatePublished - 2016

Fingerprint

Nanofibers
Vaccines
Peptides
Mucus
Composite materials
Mucosal Immunity
Antibodies
Antibody Formation
Stomach
Vaccination
Cholera Toxin
Pathogens
Antigens
Biocompatibility
Particle Size
Particle size analysis
Gold
Immunoglobulin A
Minerals
Oral Administration

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)
  • Chemistry(all)
  • Materials Science(all)

Cite this

Peptide nanofiber-CaCO3 composite microparticles as adjuvant-free oral vaccine delivery vehicles. / Snook, Joshua D.; Chesson, Charles B.; Peniche-Trujillo, Alex-Giovanny; Dann-Grice, Sara; Paulucci, Adriana; Pinchuk, Iryna; Rudra, Jai S.

In: Journal of Materials Chemistry B, Vol. 4, No. 9, 2016, p. 1640-1649.

Research output: Contribution to journalArticle

Snook, Joshua D. ; Chesson, Charles B. ; Peniche-Trujillo, Alex-Giovanny ; Dann-Grice, Sara ; Paulucci, Adriana ; Pinchuk, Iryna ; Rudra, Jai S. / Peptide nanofiber-CaCO3 composite microparticles as adjuvant-free oral vaccine delivery vehicles. In: Journal of Materials Chemistry B. 2016 ; Vol. 4, No. 9. pp. 1640-1649.
@article{58d8367ff819494e85a4fb399b27a40b,
title = "Peptide nanofiber-CaCO3 composite microparticles as adjuvant-free oral vaccine delivery vehicles",
abstract = "To combat mucosal pathogens that cause gastrointestinal (GI) infections, local mucosal immunity is required which is best achieved through oral vaccination. Oral delivery of vaccines is also a safe and convenient alternative to injected vaccines due to its non-invasive nature and high compliance rate for all ages. However, the lack of effective and safe mucosal adjuvants, the selective permeability of the mucus barrier, and the harsh GI environment continue to pose a significant challenge for oral vaccine development. Microparticle-based strategies are attractive for oral vaccination due to their ability to efficiently penetrate the mucus barrier and have the added advantage of protecting the antigen in the harsh gastric environment. In this work, self-adjuvanting peptide nanofiber-CaCO3 composite microparticles were prepared and investigated for oral vaccine delivery. Compared to polymeric microparticles, inorganic CaCO3 microparticles have unique advantages due to the biocompatibility of CaCO3 as a natural mineral, mild preparation conditions, and its porous structure that is suitable for loading other materials. Particle size distribution, nanofiber loading efficiency, morphology, and degradation in simulated gastric fluid were characterized. The composite microparticles were efficient at penetrating the mucus barrier and were localized to immune inductive sites and elicited the production of mucosal antibody responses, particularly the protective IgA isotype following oral administration. The magnitude of the mucosal immune response was comparable to the gold-standard adjuvant cholera toxin B (CTB). Our results indicate that OVA-KFE8/CaCO3 composite microparticles are efficient self-adjuvanting oral vaccine delivery vehicles for induction of mucosal antibody responses.",
author = "Snook, {Joshua D.} and Chesson, {Charles B.} and Alex-Giovanny Peniche-Trujillo and Sara Dann-Grice and Adriana Paulucci and Iryna Pinchuk and Rudra, {Jai S.}",
year = "2016",
doi = "10.1039/c5tb01623a",
language = "English (US)",
volume = "4",
pages = "1640--1649",
journal = "Journal of Materials Chemistry B",
issn = "2050-7518",
publisher = "Royal Society of Chemistry",
number = "9",

}

TY - JOUR

T1 - Peptide nanofiber-CaCO3 composite microparticles as adjuvant-free oral vaccine delivery vehicles

AU - Snook, Joshua D.

AU - Chesson, Charles B.

AU - Peniche-Trujillo, Alex-Giovanny

AU - Dann-Grice, Sara

AU - Paulucci, Adriana

AU - Pinchuk, Iryna

AU - Rudra, Jai S.

PY - 2016

Y1 - 2016

N2 - To combat mucosal pathogens that cause gastrointestinal (GI) infections, local mucosal immunity is required which is best achieved through oral vaccination. Oral delivery of vaccines is also a safe and convenient alternative to injected vaccines due to its non-invasive nature and high compliance rate for all ages. However, the lack of effective and safe mucosal adjuvants, the selective permeability of the mucus barrier, and the harsh GI environment continue to pose a significant challenge for oral vaccine development. Microparticle-based strategies are attractive for oral vaccination due to their ability to efficiently penetrate the mucus barrier and have the added advantage of protecting the antigen in the harsh gastric environment. In this work, self-adjuvanting peptide nanofiber-CaCO3 composite microparticles were prepared and investigated for oral vaccine delivery. Compared to polymeric microparticles, inorganic CaCO3 microparticles have unique advantages due to the biocompatibility of CaCO3 as a natural mineral, mild preparation conditions, and its porous structure that is suitable for loading other materials. Particle size distribution, nanofiber loading efficiency, morphology, and degradation in simulated gastric fluid were characterized. The composite microparticles were efficient at penetrating the mucus barrier and were localized to immune inductive sites and elicited the production of mucosal antibody responses, particularly the protective IgA isotype following oral administration. The magnitude of the mucosal immune response was comparable to the gold-standard adjuvant cholera toxin B (CTB). Our results indicate that OVA-KFE8/CaCO3 composite microparticles are efficient self-adjuvanting oral vaccine delivery vehicles for induction of mucosal antibody responses.

AB - To combat mucosal pathogens that cause gastrointestinal (GI) infections, local mucosal immunity is required which is best achieved through oral vaccination. Oral delivery of vaccines is also a safe and convenient alternative to injected vaccines due to its non-invasive nature and high compliance rate for all ages. However, the lack of effective and safe mucosal adjuvants, the selective permeability of the mucus barrier, and the harsh GI environment continue to pose a significant challenge for oral vaccine development. Microparticle-based strategies are attractive for oral vaccination due to their ability to efficiently penetrate the mucus barrier and have the added advantage of protecting the antigen in the harsh gastric environment. In this work, self-adjuvanting peptide nanofiber-CaCO3 composite microparticles were prepared and investigated for oral vaccine delivery. Compared to polymeric microparticles, inorganic CaCO3 microparticles have unique advantages due to the biocompatibility of CaCO3 as a natural mineral, mild preparation conditions, and its porous structure that is suitable for loading other materials. Particle size distribution, nanofiber loading efficiency, morphology, and degradation in simulated gastric fluid were characterized. The composite microparticles were efficient at penetrating the mucus barrier and were localized to immune inductive sites and elicited the production of mucosal antibody responses, particularly the protective IgA isotype following oral administration. The magnitude of the mucosal immune response was comparable to the gold-standard adjuvant cholera toxin B (CTB). Our results indicate that OVA-KFE8/CaCO3 composite microparticles are efficient self-adjuvanting oral vaccine delivery vehicles for induction of mucosal antibody responses.

UR - http://www.scopus.com/inward/record.url?scp=84959423228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959423228&partnerID=8YFLogxK

U2 - 10.1039/c5tb01623a

DO - 10.1039/c5tb01623a

M3 - Article

AN - SCOPUS:84959423228

VL - 4

SP - 1640

EP - 1649

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-7518

IS - 9

ER -