Peptide YY antagonizes β-adrenergic-stimulated release of insulin in dogs

G. H. Greeley, F. Lluis, Guillermo Gomez, J. Ishizuka, B. Holland, J. C. Thompson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Peptide YY (PYY) and neuropeptide Y (NPY) are peptides of 36 amino acids that share structural homologies with pancreatic polypeptide (PP). PP is predominantly found in the endocrine pancreas. PYY is primarily found in mucosal endocrine cells of the distal ileum, colon, and rectum, whereas NPY is found in both the peripheral and central nervous systems. Previous studies indicate that these peptides can interact with the autonomic nervous system. The objective of the present experiments was to study the effect of PYY on neurally stimulated insulin release [i.e., in response to 2-deoxy-D-glucose (2-DG), a nonmetabolizable glucose analogue] in conscious dogs. Intravenous administration of PYY [100, 200, and 400 pmol·kg-1·h-1) reduced 2-DG-stimulated insulin release in a dose-dependent manner (P<0.05) without affecting plasma glucose levels. Administration of NPY (800 pmol·kg-1·h-1), but not PP (400 pmol·kg-1·h-1), reduced 2-DG-stimulated release of insulin (P<0.05). The inhibitory action of PYY on 2-DG-stimulated insulin release persisted in the presence of atropine or phentolamine treatment; however, hexamethonium alone or phentolamine plus propranolol treatment blocked the inhibitory action of PYY. Release of insulin stimulated by the β-gonist isoproterenol was also inhibited by PYY (P<0.05). These results indicate that PYY can inhibit autonomic neurotransmission by a mechanism that may involve ganglionic or postganglionic inhibition of β-adrenergic stimulation. Our findings suggest a role for PYY and NPY in the autonomic regulation of insulin release.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume254
Issue number4
StatePublished - 1988

Fingerprint

Peptide YY
Adrenergic Agents
Dogs
Insulin
Neuropeptide Y
Deoxyglucose
Pancreatic Polypeptide
Phentolamine
Neurology
Glucose
Hexamethonium
Peptides
Endocrine Cells
Autonomic Nervous System
Peripheral Nervous System
Islets of Langerhans
Atropine
Ileum
Isoproterenol
Rectum

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

Greeley, G. H., Lluis, F., Gomez, G., Ishizuka, J., Holland, B., & Thompson, J. C. (1988). Peptide YY antagonizes β-adrenergic-stimulated release of insulin in dogs. American Journal of Physiology - Endocrinology and Metabolism, 254(4).

Peptide YY antagonizes β-adrenergic-stimulated release of insulin in dogs. / Greeley, G. H.; Lluis, F.; Gomez, Guillermo; Ishizuka, J.; Holland, B.; Thompson, J. C.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 254, No. 4, 1988.

Research output: Contribution to journalArticle

Greeley, GH, Lluis, F, Gomez, G, Ishizuka, J, Holland, B & Thompson, JC 1988, 'Peptide YY antagonizes β-adrenergic-stimulated release of insulin in dogs', American Journal of Physiology - Endocrinology and Metabolism, vol. 254, no. 4.
Greeley, G. H. ; Lluis, F. ; Gomez, Guillermo ; Ishizuka, J. ; Holland, B. ; Thompson, J. C. / Peptide YY antagonizes β-adrenergic-stimulated release of insulin in dogs. In: American Journal of Physiology - Endocrinology and Metabolism. 1988 ; Vol. 254, No. 4.
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