Peptidomics of CNS-associated neurohemal systems of adult Drosophila melanogaster

A mass spectrometric survey of peptides from individual flies

Reinhard Predel, Christian Wegener, William Russell, Shane E. Tichy, David H. Russell, Ronald J. Nachman

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Neuropeptides are important messenger molecules that influence nearly all physiological processes. In insects, they can be released as neuromodulators within the central nervous system (CNS) or as neurohormones into the hemolymph. We analyzed the peptidome of neurohormonal release sites and associated secretory peptidergic neurons of adult Drosophila melanogaster. MALDI-TOF mass spectrometric analyzes were performed on single organs or cell cluster from individual flies. This first peptidomic characterization in adult fruit flies revealed 32 different neuropeptides. Peptides not directly predictable from previously cloned or annotated precursor genes were sequenced by tandem mass spectrometry. These peptides turned out to be either intermediate products of neuropeptide processing or shorter versions of known peptides. We found that the peptidome of the CNS-associated neurohemal organs is tagma-specific in Drosophila. Abdominal neurohemal organs and their supplying peptidergic neurons contain the capa gene products periviscerokinins and pyrokinin-1, thoracic neurohemal organs contain FMRFamides, and the neurohemal release sites of the brain contain pyrokinin-12-15, pyrokinin-2, corazonin, myosuppressin, and sNPF as their major putative release products. Our results show that peptidomic approaches are well suited to study differential neuropeptide expression or posttranslational modifications in morphologically defined parts of the nervous system and in a developmental and physiological context in animals as small as Drosophila melanogaster.

Original languageEnglish (US)
Pages (from-to)379-392
Number of pages14
JournalJournal of Comparative Neurology
Volume474
Issue number3
DOIs
StatePublished - Jun 28 2004
Externally publishedYes

Fingerprint

Neuropeptides
Drosophila melanogaster
Diptera
Central Nervous System
Peptides
Neurotransmitter Agents
FMRFamide
Physiological Phenomena
Neurons
Hemolymph
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Post Translational Protein Processing
Tandem Mass Spectrometry
Nervous System
Genes
Drosophila
Insects
Fruit
Thorax
Surveys and Questionnaires

Keywords

  • ESI-Q-TOF
  • Immunocytochemistry
  • Insect
  • MALDI-TOF mass spectrometry
  • Perisympathetic organs
  • Retrocerebral complex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Peptidomics of CNS-associated neurohemal systems of adult Drosophila melanogaster : A mass spectrometric survey of peptides from individual flies. / Predel, Reinhard; Wegener, Christian; Russell, William; Tichy, Shane E.; Russell, David H.; Nachman, Ronald J.

In: Journal of Comparative Neurology, Vol. 474, No. 3, 28.06.2004, p. 379-392.

Research output: Contribution to journalArticle

Predel, Reinhard ; Wegener, Christian ; Russell, William ; Tichy, Shane E. ; Russell, David H. ; Nachman, Ronald J. / Peptidomics of CNS-associated neurohemal systems of adult Drosophila melanogaster : A mass spectrometric survey of peptides from individual flies. In: Journal of Comparative Neurology. 2004 ; Vol. 474, No. 3. pp. 379-392.
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