Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin

6 Scopus citations

Abstract

Objectives: We evaluate the performance characteristics of antiphosphatidylserine (anti-PS), antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti-β2 glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)-related diseases. Methods: Serum samples from 548 patients from the Hopkins and Jamaican systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid Standardization Laboratory were examined for immunoglobulin G (IgG)/immunoglobulin M (IgM) positivity in aCL, anti-β2GPI, anti-PS, anti-PI, and APhL ELISA assays. Results: All IgG assays were associated with one or more thrombotic and/or obstetric manifestations, with an increased risk associated with higher antibody titers. Analytical performance was similar among assays, but IgG assays performed better than IgM counterparts. Conclusions: Increasing titers of APhL, anti-PS, and anti-PI antibodies could indicate an increased risk of thrombotic and/or obstetric aPL-related manifestations. These assays may be promising biomarkers for particular APS manifestations.

Original languageEnglish (US)
Pages (from-to)401-411
Number of pages11
JournalAmerican Journal of Clinical Pathology
Volume149
Issue number5
DOIs
StatePublished - Mar 29 2018

Keywords

  • Anticardiolipin
  • Antiphosphatidylinositol
  • Antiphosphatidylserine
  • Antiphospholipid
  • APhL
  • Assay
  • Noncriteria

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin. / Castanon, Amaris; Pierre, Grant; Willis, Rohan; Nigel Harris, E.; Papalardo, Elizabeth; Romay-Penabad, Zurina; Schleh, Alvaro; Jajoria, Praveen; Smikle, Monica; Deceulaer, Karel; Tebo, Anne; Jaskowski, Troy; Guerra, Marta M.; Ware Branch, D.; Salmon, Jane E.; Petri, Michelle; Gonzalez, Emilio.

In: American Journal of Clinical Pathology, Vol. 149, No. 5, 29.03.2018, p. 401-411.

Research output: Contribution to journalArticle