Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin

Amaris Castanon, Grant Pierre, Rohan Willis, E. Nigel Harris, Elizabeth Papalardo, Zurina Romay-Penabad, Alvaro Schleh, Praveen Jajoria, Monica Smikle, Karel Deceulaer, Anne Tebo, Troy Jaskowski, Marta M. Guerra, D. Ware Branch, Jane E. Salmon, Michelle Petri, Emilio Gonzalez

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Objectives: We evaluate the performance characteristics of antiphosphatidylserine (anti-PS), antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti-β2 glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)-related diseases. Methods: Serum samples from 548 patients from the Hopkins and Jamaican systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid Standardization Laboratory were examined for immunoglobulin G (IgG)/immunoglobulin M (IgM) positivity in aCL, anti-β2GPI, anti-PS, anti-PI, and APhL ELISA assays. Results: All IgG assays were associated with one or more thrombotic and/or obstetric manifestations, with an increased risk associated with higher antibody titers. Analytical performance was similar among assays, but IgG assays performed better than IgM counterparts. Conclusions: Increasing titers of APhL, anti-PS, and anti-PI antibodies could indicate an increased risk of thrombotic and/or obstetric aPL-related manifestations. These assays may be promising biomarkers for particular APS manifestations.

Original languageEnglish (US)
Pages (from-to)401-411
Number of pages11
JournalAmerican Journal of Clinical Pathology
Volume149
Issue number5
DOIs
StatePublished - Mar 29 2018

Keywords

  • Anticardiolipin
  • Antiphosphatidylinositol
  • Antiphosphatidylserine
  • Antiphospholipid
  • APhL
  • Assay
  • Noncriteria

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin'. Together they form a unique fingerprint.

  • Cite this

    Castanon, A., Pierre, G., Willis, R., Nigel Harris, E., Papalardo, E., Romay-Penabad, Z., Schleh, A., Jajoria, P., Smikle, M., Deceulaer, K., Tebo, A., Jaskowski, T., Guerra, M. M., Ware Branch, D., Salmon, J. E., Petri, M., & Gonzalez, E. (2018). Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin. American Journal of Clinical Pathology, 149(5), 401-411. https://doi.org/10.1093/ajcp/aqy003