Performance evaluation of Puritan® universal transport system (UniTranz-RT™) for preservation and transport of clinical viruses

Trevor Brasel, Kunapuli T. Madhusudhan, Krystle Agans, Karen Dearen, Sara L. Jones, Robert L. Sherwood

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ability of a non-propagating microbial transport medium to maintain the viability of clinically relevant viruses was compared to a similar commercial medium to establish performance equivalence. Two dilutions of stock of test viruses, namely adenovirus (AdV), cytomegalovirus (CMV), echovirus Type 30 (EV), herpes simplex virus (HSV) types 1 and 2, influenza A, parainfluenza 3 (PIV), respiratory syncytial virus (RSV), and varicella zoster virus (VZV), were spiked into Puritan® Medical Products Company Universal Transport System (UniTranz-RT™) and BD<sup>TM</sup> Universal Viral Transport System (UVT) and incubated at 4°C and room temperature (RT) for up to 72hr. Post incubation assessment of recovery of AdV, EV, HSV-2, PIV, and VZV from UniTranz-RT™ and UVT using shell vial assays followed by immunofluorescence staining demonstrated statistically significant differences between both transport media. In general, significantly higher recoveries of AdV, EV, and VZV were found from UniTranz-RT™ than UVT whereas HSV-2 and PIV were recovered better from UVT than UniTranz-RT™, under specific test conditions. The recovery of HSV-1, influenza A, PIV, and RSV showed no significant differences between transport media. Sulforhodamine B-based assay analysis of UniTranz-RT™ lots prior to and at expiration exhibited no cytotoxicity. The overall results of the study validate the full performance of UniTranz-RT™ as a viral transport medium and establish its effectiveness on par with the UVT.

Original languageEnglish (US)
Pages (from-to)1796-1805
Number of pages10
JournalJournal of Medical Virology
Volume87
Issue number10
DOIs
StatePublished - Oct 1 2015

Fingerprint

Viruses
Temperature
Human Herpesvirus 3
Human Herpesvirus 2
Adenoviridae
Respiratory Syncytial Viruses
Human Herpesvirus 1
lissamine rhodamine B
Human Influenza
Human Enterovirus B
Paramyxoviridae Infections
Cytomegalovirus
Fluorescent Antibody Technique
Staining and Labeling

Keywords

  • Cytotoxicity
  • Pre-analytics
  • Transport media
  • UniTranz-RT™
  • UVT
  • Viruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Performance evaluation of Puritan® universal transport system (UniTranz-RT™) for preservation and transport of clinical viruses. / Brasel, Trevor; Madhusudhan, Kunapuli T.; Agans, Krystle; Dearen, Karen; Jones, Sara L.; Sherwood, Robert L.

In: Journal of Medical Virology, Vol. 87, No. 10, 01.10.2015, p. 1796-1805.

Research output: Contribution to journalArticle

Brasel, Trevor ; Madhusudhan, Kunapuli T. ; Agans, Krystle ; Dearen, Karen ; Jones, Sara L. ; Sherwood, Robert L. / Performance evaluation of Puritan® universal transport system (UniTranz-RT™) for preservation and transport of clinical viruses. In: Journal of Medical Virology. 2015 ; Vol. 87, No. 10. pp. 1796-1805.
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