Performance evaluation of two microbial transport media designed for preservation and transport of Chlamydiae, Mycoplasma and Ureaplasma

Sara L. Jones, Kunapuli T. Madhusudhan, Krystle Agans, Karen Dearen, Jennifer Knight, Trevor Brasel, Mehdi Karamchi, Robert L. Sherwood

Research output: Contribution to journalArticle

Abstract

The ability of a non-propagating transport device (test device) to maintain the viability of clinically relevant bacteria was compared with a similar commercial device (predicate device) to establish performance equivalence. Test bacteria, namely Chlamydia trachomatis, Chlamydia pneumoniae, Mycoplasma hominis, Mycoplasma pneumoniae and Ureaplasma urealyticum, were inoculated into the test [Puritan Medical Products Universal Transport System (UniTranz-RT<sup>TM</sup>)] and predicate (BD Universal Viral Transport System) devices, and incubated at 4 6C and room temperature for up to 72 h. Bacterial viability was assessed at selected time points postincubation using shell vial assays followed by immunofluorescence staining (for Chlamydia) or by standard culture techniques (for Mycoplasma and Ureaplasma). Results indicated that the Chlamydia strains were equally stable in both test and predicate devices through 72 h storage, at both test temperatures. Quantifiable levels of Mycoplasma and Ureaplasma were recovered from the test and predicate devices throughout the storage period. Low-temperature storage improved bacterial viability when compared with room temperature storage. In addition, the predicate device demonstrated slightly improved performance versus the test device in the context of Mycoplasma and Ureaplasma following 72 h storage. The overall results of the study confirmed the full performance of UniTranz-RT<sup>TM</sup> as a microbial transport medium and established equal performance with the predicate device.

Original languageEnglish (US)
Pages (from-to)382-389
Number of pages8
JournalJournal of Medical Microbiology
Volume64
Issue number4
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Ureaplasma
Chlamydia
Mycoplasma
Equipment and Supplies
Microbial Viability
Temperature
Mycoplasma hominis
Ureaplasma urealyticum
Bacteria
Culture Techniques
Chlamydophila pneumoniae
Mycoplasma pneumoniae
Chlamydia trachomatis
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Performance evaluation of two microbial transport media designed for preservation and transport of Chlamydiae, Mycoplasma and Ureaplasma. / Jones, Sara L.; Madhusudhan, Kunapuli T.; Agans, Krystle; Dearen, Karen; Knight, Jennifer; Brasel, Trevor; Karamchi, Mehdi; Sherwood, Robert L.

In: Journal of Medical Microbiology, Vol. 64, No. 4, 2015, p. 382-389.

Research output: Contribution to journalArticle

Jones, SL, Madhusudhan, KT, Agans, K, Dearen, K, Knight, J, Brasel, T, Karamchi, M & Sherwood, RL 2015, 'Performance evaluation of two microbial transport media designed for preservation and transport of Chlamydiae, Mycoplasma and Ureaplasma', Journal of Medical Microbiology, vol. 64, no. 4, pp. 382-389. https://doi.org/10.1099/jmm.0.000044
Jones, Sara L. ; Madhusudhan, Kunapuli T. ; Agans, Krystle ; Dearen, Karen ; Knight, Jennifer ; Brasel, Trevor ; Karamchi, Mehdi ; Sherwood, Robert L. / Performance evaluation of two microbial transport media designed for preservation and transport of Chlamydiae, Mycoplasma and Ureaplasma. In: Journal of Medical Microbiology. 2015 ; Vol. 64, No. 4. pp. 382-389.
@article{42adc615e22044499c0e423e6d6300bc,
title = "Performance evaluation of two microbial transport media designed for preservation and transport of Chlamydiae, Mycoplasma and Ureaplasma",
abstract = "The ability of a non-propagating transport device (test device) to maintain the viability of clinically relevant bacteria was compared with a similar commercial device (predicate device) to establish performance equivalence. Test bacteria, namely Chlamydia trachomatis, Chlamydia pneumoniae, Mycoplasma hominis, Mycoplasma pneumoniae and Ureaplasma urealyticum, were inoculated into the test [Puritan Medical Products Universal Transport System (UniTranz-RTTM)] and predicate (BD Universal Viral Transport System) devices, and incubated at 4 6C and room temperature for up to 72 h. Bacterial viability was assessed at selected time points postincubation using shell vial assays followed by immunofluorescence staining (for Chlamydia) or by standard culture techniques (for Mycoplasma and Ureaplasma). Results indicated that the Chlamydia strains were equally stable in both test and predicate devices through 72 h storage, at both test temperatures. Quantifiable levels of Mycoplasma and Ureaplasma were recovered from the test and predicate devices throughout the storage period. Low-temperature storage improved bacterial viability when compared with room temperature storage. In addition, the predicate device demonstrated slightly improved performance versus the test device in the context of Mycoplasma and Ureaplasma following 72 h storage. The overall results of the study confirmed the full performance of UniTranz-RTTM as a microbial transport medium and established equal performance with the predicate device.",
author = "Jones, {Sara L.} and Madhusudhan, {Kunapuli T.} and Krystle Agans and Karen Dearen and Jennifer Knight and Trevor Brasel and Mehdi Karamchi and Sherwood, {Robert L.}",
year = "2015",
doi = "10.1099/jmm.0.000044",
language = "English (US)",
volume = "64",
pages = "382--389",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "4",

}

TY - JOUR

T1 - Performance evaluation of two microbial transport media designed for preservation and transport of Chlamydiae, Mycoplasma and Ureaplasma

AU - Jones, Sara L.

AU - Madhusudhan, Kunapuli T.

AU - Agans, Krystle

AU - Dearen, Karen

AU - Knight, Jennifer

AU - Brasel, Trevor

AU - Karamchi, Mehdi

AU - Sherwood, Robert L.

PY - 2015

Y1 - 2015

N2 - The ability of a non-propagating transport device (test device) to maintain the viability of clinically relevant bacteria was compared with a similar commercial device (predicate device) to establish performance equivalence. Test bacteria, namely Chlamydia trachomatis, Chlamydia pneumoniae, Mycoplasma hominis, Mycoplasma pneumoniae and Ureaplasma urealyticum, were inoculated into the test [Puritan Medical Products Universal Transport System (UniTranz-RTTM)] and predicate (BD Universal Viral Transport System) devices, and incubated at 4 6C and room temperature for up to 72 h. Bacterial viability was assessed at selected time points postincubation using shell vial assays followed by immunofluorescence staining (for Chlamydia) or by standard culture techniques (for Mycoplasma and Ureaplasma). Results indicated that the Chlamydia strains were equally stable in both test and predicate devices through 72 h storage, at both test temperatures. Quantifiable levels of Mycoplasma and Ureaplasma were recovered from the test and predicate devices throughout the storage period. Low-temperature storage improved bacterial viability when compared with room temperature storage. In addition, the predicate device demonstrated slightly improved performance versus the test device in the context of Mycoplasma and Ureaplasma following 72 h storage. The overall results of the study confirmed the full performance of UniTranz-RTTM as a microbial transport medium and established equal performance with the predicate device.

AB - The ability of a non-propagating transport device (test device) to maintain the viability of clinically relevant bacteria was compared with a similar commercial device (predicate device) to establish performance equivalence. Test bacteria, namely Chlamydia trachomatis, Chlamydia pneumoniae, Mycoplasma hominis, Mycoplasma pneumoniae and Ureaplasma urealyticum, were inoculated into the test [Puritan Medical Products Universal Transport System (UniTranz-RTTM)] and predicate (BD Universal Viral Transport System) devices, and incubated at 4 6C and room temperature for up to 72 h. Bacterial viability was assessed at selected time points postincubation using shell vial assays followed by immunofluorescence staining (for Chlamydia) or by standard culture techniques (for Mycoplasma and Ureaplasma). Results indicated that the Chlamydia strains were equally stable in both test and predicate devices through 72 h storage, at both test temperatures. Quantifiable levels of Mycoplasma and Ureaplasma were recovered from the test and predicate devices throughout the storage period. Low-temperature storage improved bacterial viability when compared with room temperature storage. In addition, the predicate device demonstrated slightly improved performance versus the test device in the context of Mycoplasma and Ureaplasma following 72 h storage. The overall results of the study confirmed the full performance of UniTranz-RTTM as a microbial transport medium and established equal performance with the predicate device.

UR - http://www.scopus.com/inward/record.url?scp=84927732718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927732718&partnerID=8YFLogxK

U2 - 10.1099/jmm.0.000044

DO - 10.1099/jmm.0.000044

M3 - Article

C2 - 25713205

AN - SCOPUS:84927732718

VL - 64

SP - 382

EP - 389

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 4

ER -