TY - JOUR
T1 - Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season
AU - Rahman, Mahbubur
AU - Vandermause, Mary F.
AU - Kieke, Burney A.
AU - Belongia, Edward A.
PY - 2008/10
Y1 - 2008/10
N2 - The Binax NOW Flu A and Flu B (Binax NOW), direct fluorescent assay (DFA), and viral culture were evaluated and compared with a composite of viral culture or reverse transcription polymerase chain reaction (RT-PCR). Participants with medically attended acute respiratory illness were identified through active surveillance during the 2006 to 2007 season, and consenting individuals (n = 932) were tested for influenza by culture and RT-PCR. Physicians ordered a rapid antigen test (Binax NOW [n = 73] or DFA [n = 70]) according to their clinical judgment. The Binax NOW detected 11 of 18 influenza infections (sensitivity, 61%; 95% confidence interval [CI], 36-83%), whereas DFA detected 17 of 21 influenza infections (sensitivity 81%, 95% CI, 58-95%). Compared with culture/RT-PCR, specificity of both Binax NOW and DFA was 100%. During the 2006 to 2007 influenza season, DFA and Binax NOW demonstrated high specificity but failed to identify a substantial proportion of influenza infections.
AB - The Binax NOW Flu A and Flu B (Binax NOW), direct fluorescent assay (DFA), and viral culture were evaluated and compared with a composite of viral culture or reverse transcription polymerase chain reaction (RT-PCR). Participants with medically attended acute respiratory illness were identified through active surveillance during the 2006 to 2007 season, and consenting individuals (n = 932) were tested for influenza by culture and RT-PCR. Physicians ordered a rapid antigen test (Binax NOW [n = 73] or DFA [n = 70]) according to their clinical judgment. The Binax NOW detected 11 of 18 influenza infections (sensitivity, 61%; 95% confidence interval [CI], 36-83%), whereas DFA detected 17 of 21 influenza infections (sensitivity 81%, 95% CI, 58-95%). Compared with culture/RT-PCR, specificity of both Binax NOW and DFA was 100%. During the 2006 to 2007 influenza season, DFA and Binax NOW demonstrated high specificity but failed to identify a substantial proportion of influenza infections.
KW - Binax NOW
KW - Direct fluorescent assay
KW - Influenza virus
KW - Rapid test
KW - Sensitivity
KW - Specificity
UR - https://www.scopus.com/pages/publications/51649115039
UR - https://www.scopus.com/pages/publications/51649115039#tab=citedBy
U2 - 10.1016/j.diagmicrobio.2007.10.012
DO - 10.1016/j.diagmicrobio.2007.10.012
M3 - Article
C2 - 18060723
AN - SCOPUS:51649115039
SN - 0732-8893
VL - 62
SP - 162
EP - 166
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 2
ER -