Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season

Mahbubur Rahman; Mary F. Vandermause; Burney A. Kieke; Edward A. Belongia

doi:10.1016/j.diagmicrobio.2007.10.012

Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season

Mahbubur Rahman
, Mary F. Vandermause
, Burney A. Kieke
, Edward A. Belongia

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The Binax NOW Flu A and Flu B (Binax NOW), direct fluorescent assay (DFA), and viral culture were evaluated and compared with a composite of viral culture or reverse transcription polymerase chain reaction (RT-PCR). Participants with medically attended acute respiratory illness were identified through active surveillance during the 2006 to 2007 season, and consenting individuals (n = 932) were tested for influenza by culture and RT-PCR. Physicians ordered a rapid antigen test (Binax NOW [n = 73] or DFA [n = 70]) according to their clinical judgment. The Binax NOW detected 11 of 18 influenza infections (sensitivity, 61%; 95% confidence interval [CI], 36-83%), whereas DFA detected 17 of 21 influenza infections (sensitivity 81%, 95% CI, 58-95%). Compared with culture/RT-PCR, specificity of both Binax NOW and DFA was 100%. During the 2006 to 2007 influenza season, DFA and Binax NOW demonstrated high specificity but failed to identify a substantial proportion of influenza infections.

Original language	English (US)
Pages (from-to)	162-166
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	62
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2007.10.012
State	Published - Oct 2008

Keywords

Binax NOW
Direct fluorescent assay
Influenza virus
Rapid test
Sensitivity
Specificity

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2007.10.012

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Rahman, M., Vandermause, M. F., Kieke, B. A., & Belongia, E. A. (2008). Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season. Diagnostic Microbiology and Infectious Disease, 62(2), 162-166. https://doi.org/10.1016/j.diagmicrobio.2007.10.012

Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season. / Rahman, Mahbubur; Vandermause, Mary F.; Kieke, Burney A. et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 62, No. 2, 10.2008, p. 162-166.

Research output: Contribution to journal › Article › peer-review

Rahman, M, Vandermause, MF, Kieke, BA & Belongia, EA 2008, 'Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season', Diagnostic Microbiology and Infectious Disease, vol. 62, no. 2, pp. 162-166. https://doi.org/10.1016/j.diagmicrobio.2007.10.012

Rahman M, Vandermause MF, Kieke BA, Belongia EA. Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season. Diagnostic Microbiology and Infectious Disease. 2008 Oct;62(2):162-166. doi: 10.1016/j.diagmicrobio.2007.10.012

Rahman, Mahbubur ; Vandermause, Mary F. ; Kieke, Burney A. et al. / Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season. In: Diagnostic Microbiology and Infectious Disease. 2008 ; Vol. 62, No. 2. pp. 162-166.

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abstract = "The Binax NOW Flu A and Flu B (Binax NOW), direct fluorescent assay (DFA), and viral culture were evaluated and compared with a composite of viral culture or reverse transcription polymerase chain reaction (RT-PCR). Participants with medically attended acute respiratory illness were identified through active surveillance during the 2006 to 2007 season, and consenting individuals (n = 932) were tested for influenza by culture and RT-PCR. Physicians ordered a rapid antigen test (Binax NOW [n = 73] or DFA [n = 70]) according to their clinical judgment. The Binax NOW detected 11 of 18 influenza infections (sensitivity, 61\%; 95\% confidence interval [CI], 36-83\%), whereas DFA detected 17 of 21 influenza infections (sensitivity 81\%, 95\% CI, 58-95\%). Compared with culture/RT-PCR, specificity of both Binax NOW and DFA was 100\%. During the 2006 to 2007 influenza season, DFA and Binax NOW demonstrated high specificity but failed to identify a substantial proportion of influenza infections.",

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Performance of Binax NOW Flu A and B and direct fluorescent assay in comparison with a composite of viral culture or reverse transcription polymerase chain reaction for detection of influenza infection during the 2006 to 2007 season

Abstract

Keywords

ASJC Scopus subject areas

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