Performance of Directigen flu A+B enzyme immunoassay and direct fluorescent assay for detection of influenza infection during the 2004-2005 season

Mahbubur Rahman, Burney A. Kieke, Mary F. Vandermause, Paul D. Mitchell, Robert T. Greenlee, Edward A. Belongia

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Early diagnosis of influenza infection is needed to optimize the benefit of prescribing antiviral drugs. However, the accuracy of rapid tests is highly variable. This study evaluated the performance of Directigen flu A+B enzyme immunoassay (EIA) and direct fluorescent assay (DFA) during the 2004-2005 influenza season. Participants with medically attended acute respiratory illness were identified through an active surveillance. Consenting patients (n = 818) were enrolled and cultured for influenza. Physicians ordered a rapid antigen test (EIA or DFA) according to their clinical judgment. Physicians ordered rapid tests with EIA (n = 109), DFA (n = 86), or both (n = 9) in 204 patients with acute respiratory illness who were also cultured for influenza. The EIA detected 18 of 43 influenza infections (sensitivity, 42%; 95% confidence interval [CI], 28-57%), whereas DFA detected 26 of 38 influenza infections (sensitivity, 68%; 95% CI, 53-81%). Compared with culture, specificity of both EIA and DFA was 96%. During the 2004-2005 influenza season, both the EIA and DFA had low sensitivity and failed to detect influenza in many patients.

Original languageEnglish (US)
Pages (from-to)413-418
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume58
Issue number4
DOIs
StatePublished - Aug 2007
Externally publishedYes

Fingerprint

Immunoenzyme Techniques
Human Influenza
Infection
Confidence Intervals
Physicians
Antiviral Agents
Early Diagnosis
Antigens

Keywords

  • Direct fluorescent assay
  • Directigen enzyme immunoassay
  • Influenza virus
  • Rapid test
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Performance of Directigen flu A+B enzyme immunoassay and direct fluorescent assay for detection of influenza infection during the 2004-2005 season. / Rahman, Mahbubur; Kieke, Burney A.; Vandermause, Mary F.; Mitchell, Paul D.; Greenlee, Robert T.; Belongia, Edward A.

In: Diagnostic Microbiology and Infectious Disease, Vol. 58, No. 4, 08.2007, p. 413-418.

Research output: Contribution to journalArticle

Rahman, Mahbubur ; Kieke, Burney A. ; Vandermause, Mary F. ; Mitchell, Paul D. ; Greenlee, Robert T. ; Belongia, Edward A. / Performance of Directigen flu A+B enzyme immunoassay and direct fluorescent assay for detection of influenza infection during the 2004-2005 season. In: Diagnostic Microbiology and Infectious Disease. 2007 ; Vol. 58, No. 4. pp. 413-418.
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