Performance of MALDI-TOF mass spectrometry to determine the sex of mosquitoes and identify specific colonies from french polynesia

Fatou Kine Fall, Maureen Laroche, Herve Bossin, Didier Musso, Philippe Parola

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Mosquitoes are the main arthropod vectors of human pathogens. The current methods for mosquito identification include morphological and molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), now routinely used for bacterial identification, has recently emerged in the field of entomology. The aim of this study was to use MALDI-TOF MS to identify mosquito colonies from French Polynesia. Five hundred specimens from French Polynesia belonging to three species, Aedes aegypti, Aedes polynesiensis, and Culex quinquefasciatus, were included in the study. Testing the legs of these mosquitoes by MALDI-TOF MS revealed a 100% correct identification of all specimens at the species level. The MALDI-TOFMSprofiles obtained allowed differentiation of male from female mosquitoes and the specific identification of female mosquito colonies of the same species but different geographic origin.

Original languageEnglish (US)
Pages (from-to)1907-1916
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume104
Issue number5
DOIs
StatePublished - May 5 2021
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Performance of MALDI-TOF mass spectrometry to determine the sex of mosquitoes and identify specific colonies from french polynesia'. Together they form a unique fingerprint.

Cite this