Performance of reversed transcription loop-mediated isothermal amplification technique detecting EV71: A systematic review with meta-analysis

Xiaoying Lei, Hongling Wen, Li Zhao, Xuejie Yu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human enterovirus 71 (EV71) is the major etiological agent of hand, foot and mouth disease (HFMD), which is a common infectious disease in young children. Studies in the past have shown that reversed transcription loop-mediated isothermal amplification (RT-LAMP) was a rapid approach for the detection of EV71 in HFMD. This meta-analysis study is to evaluate the diagnostic role of RT-LAMP in detecting EV71 infection. A comprehensive literature research of PubMed, Embase, Wan Fang Data, and Chinese National Knowledge Infrastructure databases was conducted on articles aiming at the diagnostic performance of RT-LAMP in EV71 detection published before February 10, 2014. Data from selected studies were pooled to yield the summary sensitivity, specificity, positive and negative likelihood ratio (PLR, NLR), diagnostic odds ratio (DOR), and receiver operating characteristic (SROC) curve by using STATA VERSION 12.0 software. Ten studies including a total of 907 clinical samples were of high quality in this meta-analysis. Overall, the pooled sensitivity, specificity, PLR, NLR, DOR, and the area under the SROC curve was 0.99 (0.97, 1.00), 0.97 (0.94, 1.00), 5.90 (95% CI: 3.90-8.94), 0.20 (95% CI: 0.14-0.29), and 1.00 (95% CI: 0.99-1.00), respectively. The univariate analysis of potential variables showed some changes in the diagnostic performance, but none of the differences reached statistical significance. Despite inter-study variability, the test performance of RT-LAMP was consistent with real-time RT-PCR in detecting EV71. This meta-analysis suggests that RT-LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting EV71.

Original languageEnglish (US)
Pages (from-to)75-83
Number of pages9
JournalBioScience Trends
Volume8
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Enterovirus
Transcription
Amplification
Meta-Analysis
diagnostic
Hand, Foot and Mouth Disease
Sensitivity and Specificity
performance
Human Enterovirus A
Odds Ratio
Enterovirus Infections
PubMed
ROC Curve
Area Under Curve
Disease
Communicable Diseases
Real-Time Polymerase Chain Reaction
Software
statistical significance
Databases

Keywords

  • Detection
  • Human enterovirus 71 (EV71)
  • Meta-analysis
  • Reversed transcription loop-mediated isothermal amplification (RT-LAMP)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Health(social science)

Cite this

Performance of reversed transcription loop-mediated isothermal amplification technique detecting EV71 : A systematic review with meta-analysis. / Lei, Xiaoying; Wen, Hongling; Zhao, Li; Yu, Xuejie.

In: BioScience Trends, Vol. 8, No. 2, 2014, p. 75-83.

Research output: Contribution to journalArticle

@article{e4ff46293d204f9f8e915c179f7041b3,
title = "Performance of reversed transcription loop-mediated isothermal amplification technique detecting EV71: A systematic review with meta-analysis",
abstract = "Human enterovirus 71 (EV71) is the major etiological agent of hand, foot and mouth disease (HFMD), which is a common infectious disease in young children. Studies in the past have shown that reversed transcription loop-mediated isothermal amplification (RT-LAMP) was a rapid approach for the detection of EV71 in HFMD. This meta-analysis study is to evaluate the diagnostic role of RT-LAMP in detecting EV71 infection. A comprehensive literature research of PubMed, Embase, Wan Fang Data, and Chinese National Knowledge Infrastructure databases was conducted on articles aiming at the diagnostic performance of RT-LAMP in EV71 detection published before February 10, 2014. Data from selected studies were pooled to yield the summary sensitivity, specificity, positive and negative likelihood ratio (PLR, NLR), diagnostic odds ratio (DOR), and receiver operating characteristic (SROC) curve by using STATA VERSION 12.0 software. Ten studies including a total of 907 clinical samples were of high quality in this meta-analysis. Overall, the pooled sensitivity, specificity, PLR, NLR, DOR, and the area under the SROC curve was 0.99 (0.97, 1.00), 0.97 (0.94, 1.00), 5.90 (95{\%} CI: 3.90-8.94), 0.20 (95{\%} CI: 0.14-0.29), and 1.00 (95{\%} CI: 0.99-1.00), respectively. The univariate analysis of potential variables showed some changes in the diagnostic performance, but none of the differences reached statistical significance. Despite inter-study variability, the test performance of RT-LAMP was consistent with real-time RT-PCR in detecting EV71. This meta-analysis suggests that RT-LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting EV71.",
keywords = "Detection, Human enterovirus 71 (EV71), Meta-analysis, Reversed transcription loop-mediated isothermal amplification (RT-LAMP)",
author = "Xiaoying Lei and Hongling Wen and Li Zhao and Xuejie Yu",
year = "2014",
doi = "10.5582/bst.8.75",
language = "English (US)",
volume = "8",
pages = "75--83",
journal = "BioScience Trends",
issn = "1881-7815",
publisher = "International Advancement Center for Medicine & Health Research Co., Ltd. (IACMHR Co., Ltd.)",
number = "2",

}

TY - JOUR

T1 - Performance of reversed transcription loop-mediated isothermal amplification technique detecting EV71

T2 - A systematic review with meta-analysis

AU - Lei, Xiaoying

AU - Wen, Hongling

AU - Zhao, Li

AU - Yu, Xuejie

PY - 2014

Y1 - 2014

N2 - Human enterovirus 71 (EV71) is the major etiological agent of hand, foot and mouth disease (HFMD), which is a common infectious disease in young children. Studies in the past have shown that reversed transcription loop-mediated isothermal amplification (RT-LAMP) was a rapid approach for the detection of EV71 in HFMD. This meta-analysis study is to evaluate the diagnostic role of RT-LAMP in detecting EV71 infection. A comprehensive literature research of PubMed, Embase, Wan Fang Data, and Chinese National Knowledge Infrastructure databases was conducted on articles aiming at the diagnostic performance of RT-LAMP in EV71 detection published before February 10, 2014. Data from selected studies were pooled to yield the summary sensitivity, specificity, positive and negative likelihood ratio (PLR, NLR), diagnostic odds ratio (DOR), and receiver operating characteristic (SROC) curve by using STATA VERSION 12.0 software. Ten studies including a total of 907 clinical samples were of high quality in this meta-analysis. Overall, the pooled sensitivity, specificity, PLR, NLR, DOR, and the area under the SROC curve was 0.99 (0.97, 1.00), 0.97 (0.94, 1.00), 5.90 (95% CI: 3.90-8.94), 0.20 (95% CI: 0.14-0.29), and 1.00 (95% CI: 0.99-1.00), respectively. The univariate analysis of potential variables showed some changes in the diagnostic performance, but none of the differences reached statistical significance. Despite inter-study variability, the test performance of RT-LAMP was consistent with real-time RT-PCR in detecting EV71. This meta-analysis suggests that RT-LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting EV71.

AB - Human enterovirus 71 (EV71) is the major etiological agent of hand, foot and mouth disease (HFMD), which is a common infectious disease in young children. Studies in the past have shown that reversed transcription loop-mediated isothermal amplification (RT-LAMP) was a rapid approach for the detection of EV71 in HFMD. This meta-analysis study is to evaluate the diagnostic role of RT-LAMP in detecting EV71 infection. A comprehensive literature research of PubMed, Embase, Wan Fang Data, and Chinese National Knowledge Infrastructure databases was conducted on articles aiming at the diagnostic performance of RT-LAMP in EV71 detection published before February 10, 2014. Data from selected studies were pooled to yield the summary sensitivity, specificity, positive and negative likelihood ratio (PLR, NLR), diagnostic odds ratio (DOR), and receiver operating characteristic (SROC) curve by using STATA VERSION 12.0 software. Ten studies including a total of 907 clinical samples were of high quality in this meta-analysis. Overall, the pooled sensitivity, specificity, PLR, NLR, DOR, and the area under the SROC curve was 0.99 (0.97, 1.00), 0.97 (0.94, 1.00), 5.90 (95% CI: 3.90-8.94), 0.20 (95% CI: 0.14-0.29), and 1.00 (95% CI: 0.99-1.00), respectively. The univariate analysis of potential variables showed some changes in the diagnostic performance, but none of the differences reached statistical significance. Despite inter-study variability, the test performance of RT-LAMP was consistent with real-time RT-PCR in detecting EV71. This meta-analysis suggests that RT-LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting EV71.

KW - Detection

KW - Human enterovirus 71 (EV71)

KW - Meta-analysis

KW - Reversed transcription loop-mediated isothermal amplification (RT-LAMP)

UR - http://www.scopus.com/inward/record.url?scp=84899699215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899699215&partnerID=8YFLogxK

U2 - 10.5582/bst.8.75

DO - 10.5582/bst.8.75

M3 - Article

C2 - 24815384

AN - SCOPUS:84899699215

VL - 8

SP - 75

EP - 83

JO - BioScience Trends

JF - BioScience Trends

SN - 1881-7815

IS - 2

ER -