Perfusion deficit parallels exacerbation of cerebral ischemia/reperfusion injury in hyperglycemic rats

Michael J. Quast, Jingna Wei, Neng C. Huang, Donald G. Brunder, Stacy Sell, Jose M. Gonzalez, Gilbert R. Hillman, Thomas A. Kent

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Magnetic resonance imaging (MRI) techniques were used to determine the effect of preexisting hyperglycemia on the extent of cerebral ischemia/reperfusion injury and the level of cerebral perfusion. Middle cerebral artery occlusion (MCAO) was induced by a suture insertion technique. Forty one rats were divided into hyperglycemic and normoglycemic groups with either 4 hours of continuous MCAO or 2 hours of MCAO followed by 2 hours of reperfusion. Diffusion-weighted imaging (DWI) was performed at 4 hours after MCAO to quantify the degree of injury in 6 brain regions. Relative cerebral blood flow (CBF) and cerebral blood volume (CBV) were estimated using gradient echo (GE) bolus tracking and steady-state spin echo (SE) imaging techniques, respectively. Brain injury correlated with the perfusion level measured in both SE CBV and dynamic GE CBF images. In the temporary MCAO model, mean lesion size in DWI was 118% larger and hemispheric CBV was reduced by 37% in hyperglycemic compared with normoglycemic rats. Hyperglycemia did not significantly exacerbate brain injury or CBV deficit in permanent MCAO models. We conclude that preexisting hyperglycemia increases acute postischemic MRI-measurable brain cellular injury in proportion to an associated increased microvascular ischemia.

Original languageEnglish (US)
Pages (from-to)553-559
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume17
Issue number5
StatePublished - May 1997

Fingerprint

Middle Cerebral Artery Infarction
Reperfusion Injury
Brain Ischemia
Perfusion
Cerebrovascular Circulation
Hyperglycemia
Brain Injuries
Magnetic Resonance Imaging
Suture Techniques
Reperfusion
Ischemia
Cerebral Blood Volume
Wounds and Injuries
Brain

Keywords

  • cerebral ischemia
  • diffusion imaging
  • hyperglycemia
  • magnetic resonance imaging
  • perfusion imaging
  • reperfusion injury

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Quast, M. J., Wei, J., Huang, N. C., Brunder, D. G., Sell, S., Gonzalez, J. M., ... Kent, T. A. (1997). Perfusion deficit parallels exacerbation of cerebral ischemia/reperfusion injury in hyperglycemic rats. Journal of Cerebral Blood Flow and Metabolism, 17(5), 553-559.

Perfusion deficit parallels exacerbation of cerebral ischemia/reperfusion injury in hyperglycemic rats. / Quast, Michael J.; Wei, Jingna; Huang, Neng C.; Brunder, Donald G.; Sell, Stacy; Gonzalez, Jose M.; Hillman, Gilbert R.; Kent, Thomas A.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 17, No. 5, 05.1997, p. 553-559.

Research output: Contribution to journalArticle

Quast, MJ, Wei, J, Huang, NC, Brunder, DG, Sell, S, Gonzalez, JM, Hillman, GR & Kent, TA 1997, 'Perfusion deficit parallels exacerbation of cerebral ischemia/reperfusion injury in hyperglycemic rats', Journal of Cerebral Blood Flow and Metabolism, vol. 17, no. 5, pp. 553-559.
Quast, Michael J. ; Wei, Jingna ; Huang, Neng C. ; Brunder, Donald G. ; Sell, Stacy ; Gonzalez, Jose M. ; Hillman, Gilbert R. ; Kent, Thomas A. / Perfusion deficit parallels exacerbation of cerebral ischemia/reperfusion injury in hyperglycemic rats. In: Journal of Cerebral Blood Flow and Metabolism. 1997 ; Vol. 17, No. 5. pp. 553-559.
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