Perfusion in hamster skin treated with glycerol

Raiyan T. Zaman, Ashwin B. Parthasarathy, Gracie Vargas, Bo Chen, Andrew K. Dunn, Henry G. Rylander, Ashley J. Welch

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Background and Objective: The objective of this article is to quantify the effect of hyper-osmotic agent (glycerol) on blood velocity in hamster skin blood vessels measured with a dynamic imaging technique, laser speckle contrast imaging (LSCI). Study Design/Materials and Methods: In this study a dorsal skin-flap window was implanted on the hamster skin. The hyper-osmotic drug, that is, glycerol was delivered to the skin through the open dermal end of the window model. A two-dimensional map of blood flow of skin blood vessels was obtained from the speckle contrast (SC) images. Results: Preliminary studies demonstrated that hyperosmotic agents such as glycerol not only make tissue temporarily transparent, but also reduce blood flow. The blood perfusion was measured every 3 minutes for 36-66 minutes after diffusion of anhydrous glycerol. Blood flow in small capillaries was found to be reduced significantly within 3-9 minutes. Blood flow in larger blood vessels (i.e., all arteries and veins) decreased over time and some veins had significantly reduced blood flow within 36 minutes. At 24 hours, there was a further reduction in capillary blood perfusion whereas larger blood vessels regained flow compared to an hour after initial application of glycerol. Conclusion: Blood flow velocity and vessel diameter of the micro-vasculatures of hamster skin were reduced by the application of 100% anhydrous glycerol. At 24 hours, capillary perfusion remained depressed.

Original languageEnglish (US)
Pages (from-to)492-503
Number of pages12
JournalLasers in Surgery and Medicine
Issue number7
StatePublished - Sep 1 2009


  • Blood flow velocity
  • Hyper-osmotic agents
  • Laser speckle contrast imaging (LSCI)
  • Speckle contrast (SC)
  • Speckle measurement
  • Window model

ASJC Scopus subject areas

  • Surgery
  • Dermatology


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