Abstract
Background and Objective: The objective of this article is to quantify the effect of hyper-osmotic agent (glycerol) on blood velocity in hamster skin blood vessels measured with a dynamic imaging technique, laser speckle contrast imaging (LSCI). Study Design/Materials and Methods: In this study a dorsal skin-flap window was implanted on the hamster skin. The hyper-osmotic drug, that is, glycerol was delivered to the skin through the open dermal end of the window model. A two-dimensional map of blood flow of skin blood vessels was obtained from the speckle contrast (SC) images. Results: Preliminary studies demonstrated that hyperosmotic agents such as glycerol not only make tissue temporarily transparent, but also reduce blood flow. The blood perfusion was measured every 3 minutes for 36-66 minutes after diffusion of anhydrous glycerol. Blood flow in small capillaries was found to be reduced significantly within 3-9 minutes. Blood flow in larger blood vessels (i.e., all arteries and veins) decreased over time and some veins had significantly reduced blood flow within 36 minutes. At 24 hours, there was a further reduction in capillary blood perfusion whereas larger blood vessels regained flow compared to an hour after initial application of glycerol. Conclusion: Blood flow velocity and vessel diameter of the micro-vasculatures of hamster skin were reduced by the application of 100% anhydrous glycerol. At 24 hours, capillary perfusion remained depressed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 492-503 |
| Number of pages | 12 |
| Journal | Lasers in Surgery and Medicine |
| Volume | 41 |
| Issue number | 7 |
| DOIs | |
| State | Published - Sep 2009 |
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Keywords
- Blood flow velocity
- Hyper-osmotic agents
- Laser speckle contrast imaging (LSCI)
- Speckle contrast (SC)
- Speckle measurement
- Window model
ASJC Scopus subject areas
- Surgery
- Dermatology
Cite this
Perfusion in hamster skin treated with glycerol. / Zaman, Raiyan T.; Parthasarathy, Ashwin B.; Vargas, Gracie; Chen, Bo; Dunn, Andrew K.; Rylander, Henry G.; Welch, Ashley J.
In: Lasers in Surgery and Medicine, Vol. 41, No. 7, 09.2009, p. 492-503.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Perfusion in hamster skin treated with glycerol
AU - Zaman, Raiyan T.
AU - Parthasarathy, Ashwin B.
AU - Vargas, Gracie
AU - Chen, Bo
AU - Dunn, Andrew K.
AU - Rylander, Henry G.
AU - Welch, Ashley J.
PY - 2009/9
Y1 - 2009/9
N2 - Background and Objective: The objective of this article is to quantify the effect of hyper-osmotic agent (glycerol) on blood velocity in hamster skin blood vessels measured with a dynamic imaging technique, laser speckle contrast imaging (LSCI). Study Design/Materials and Methods: In this study a dorsal skin-flap window was implanted on the hamster skin. The hyper-osmotic drug, that is, glycerol was delivered to the skin through the open dermal end of the window model. A two-dimensional map of blood flow of skin blood vessels was obtained from the speckle contrast (SC) images. Results: Preliminary studies demonstrated that hyperosmotic agents such as glycerol not only make tissue temporarily transparent, but also reduce blood flow. The blood perfusion was measured every 3 minutes for 36-66 minutes after diffusion of anhydrous glycerol. Blood flow in small capillaries was found to be reduced significantly within 3-9 minutes. Blood flow in larger blood vessels (i.e., all arteries and veins) decreased over time and some veins had significantly reduced blood flow within 36 minutes. At 24 hours, there was a further reduction in capillary blood perfusion whereas larger blood vessels regained flow compared to an hour after initial application of glycerol. Conclusion: Blood flow velocity and vessel diameter of the micro-vasculatures of hamster skin were reduced by the application of 100% anhydrous glycerol. At 24 hours, capillary perfusion remained depressed.
AB - Background and Objective: The objective of this article is to quantify the effect of hyper-osmotic agent (glycerol) on blood velocity in hamster skin blood vessels measured with a dynamic imaging technique, laser speckle contrast imaging (LSCI). Study Design/Materials and Methods: In this study a dorsal skin-flap window was implanted on the hamster skin. The hyper-osmotic drug, that is, glycerol was delivered to the skin through the open dermal end of the window model. A two-dimensional map of blood flow of skin blood vessels was obtained from the speckle contrast (SC) images. Results: Preliminary studies demonstrated that hyperosmotic agents such as glycerol not only make tissue temporarily transparent, but also reduce blood flow. The blood perfusion was measured every 3 minutes for 36-66 minutes after diffusion of anhydrous glycerol. Blood flow in small capillaries was found to be reduced significantly within 3-9 minutes. Blood flow in larger blood vessels (i.e., all arteries and veins) decreased over time and some veins had significantly reduced blood flow within 36 minutes. At 24 hours, there was a further reduction in capillary blood perfusion whereas larger blood vessels regained flow compared to an hour after initial application of glycerol. Conclusion: Blood flow velocity and vessel diameter of the micro-vasculatures of hamster skin were reduced by the application of 100% anhydrous glycerol. At 24 hours, capillary perfusion remained depressed.
KW - Blood flow velocity
KW - Hyper-osmotic agents
KW - Laser speckle contrast imaging (LSCI)
KW - Speckle contrast (SC)
KW - Speckle measurement
KW - Window model
UR - http://www.scopus.com/inward/record.url?scp=69749108939&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69749108939&partnerID=8YFLogxK
U2 - 10.1002/lsm.20803
DO - 10.1002/lsm.20803
M3 - Article
C2 - 19670326
AN - SCOPUS:69749108939
VL - 41
SP - 492
EP - 503
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
SN - 0196-8092
IS - 7
ER -