Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients

Ronald Tilton, A. M. Faller, J. K. Burkhardt, P. L. Hoffmann, C. Kilo, J. R. Williamson

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Ultrastractural morphometry was used to quantify capillary basement membrane width, pericyte coverage of capillaries, pericyte degeneration, and the extent of acellular capillaries in skeletal muscle obtained at autopsy from neck, thigh, calf and foot of five male and four female diabetic subjects and an equal number of sex- and age-matched nondiabetic subjects. Within diabetic or nondiabetic subjects, the trend for all four parameters to increase in frequency or magnitude in the order neck < thigh < calf was highly significant; the only statistically significant difference between calf and foot muscles for any of the four parameters was capillary basement membrane width for nondiabetic subjects, which was significantly thinner in foot than in calf muscle (t=2.45;p<0.05). Pericyte coverage of capillaries did not differ between diabetic and nondiabetic subjects for each muscle examined; however, capillary basement membrane width, the frequency of pericyte debris and acellular capillaries were increased significantly in the lower extremity muscles of diabetic compared to nondiabetic subjects, and the magnitude of the difference between these two groups increased in the order thigh < calf < foot. The observations that pericyte degeneration and acellular capillaries are present in skeletal muscle as well as in retinal microvessels suggest that common pathophysiological mechanisms may contribute to vascular disease in these two very different tissues. The additional finding that relative differences between diabetic and nondiabetic subjects, in the frequency and magnitude of these changes, increase in the order neck < calf < foot is consistent with the marked increase in peripheral vascular disease and gangrene in the lower extremities of diabetic patients.

Original languageEnglish (US)
Pages (from-to)895-900
Number of pages6
JournalDiabetologia
Volume28
Issue number12
DOIs
StatePublished - Dec 1985
Externally publishedYes

Fingerprint

Pericytes
Diabetic Foot
Foot
Thigh
Basement Membrane
Muscles
Neck
Lower Extremity
Skeletal Muscle
Gangrene
Peripheral Vascular Diseases
Microvessels
Vascular Diseases
Autopsy

Keywords

  • acellular capillaries
  • capillary basement membrane width
  • diabetes
  • diabetic retinopathy
  • Pericyte
  • skeletal muscle

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Tilton, R., Faller, A. M., Burkhardt, J. K., Hoffmann, P. L., Kilo, C., & Williamson, J. R. (1985). Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients. Diabetologia, 28(12), 895-900. https://doi.org/10.1007/BF00703132

Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients. / Tilton, Ronald; Faller, A. M.; Burkhardt, J. K.; Hoffmann, P. L.; Kilo, C.; Williamson, J. R.

In: Diabetologia, Vol. 28, No. 12, 12.1985, p. 895-900.

Research output: Contribution to journalArticle

Tilton, R, Faller, AM, Burkhardt, JK, Hoffmann, PL, Kilo, C & Williamson, JR 1985, 'Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients', Diabetologia, vol. 28, no. 12, pp. 895-900. https://doi.org/10.1007/BF00703132
Tilton R, Faller AM, Burkhardt JK, Hoffmann PL, Kilo C, Williamson JR. Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients. Diabetologia. 1985 Dec;28(12):895-900. https://doi.org/10.1007/BF00703132
Tilton, Ronald ; Faller, A. M. ; Burkhardt, J. K. ; Hoffmann, P. L. ; Kilo, C. ; Williamson, J. R. / Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients. In: Diabetologia. 1985 ; Vol. 28, No. 12. pp. 895-900.
@article{a8632134da6341fa8eda3ca6b15844a7,
title = "Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients",
abstract = "Ultrastractural morphometry was used to quantify capillary basement membrane width, pericyte coverage of capillaries, pericyte degeneration, and the extent of acellular capillaries in skeletal muscle obtained at autopsy from neck, thigh, calf and foot of five male and four female diabetic subjects and an equal number of sex- and age-matched nondiabetic subjects. Within diabetic or nondiabetic subjects, the trend for all four parameters to increase in frequency or magnitude in the order neck < thigh < calf was highly significant; the only statistically significant difference between calf and foot muscles for any of the four parameters was capillary basement membrane width for nondiabetic subjects, which was significantly thinner in foot than in calf muscle (t=2.45;p<0.05). Pericyte coverage of capillaries did not differ between diabetic and nondiabetic subjects for each muscle examined; however, capillary basement membrane width, the frequency of pericyte debris and acellular capillaries were increased significantly in the lower extremity muscles of diabetic compared to nondiabetic subjects, and the magnitude of the difference between these two groups increased in the order thigh < calf < foot. The observations that pericyte degeneration and acellular capillaries are present in skeletal muscle as well as in retinal microvessels suggest that common pathophysiological mechanisms may contribute to vascular disease in these two very different tissues. The additional finding that relative differences between diabetic and nondiabetic subjects, in the frequency and magnitude of these changes, increase in the order neck < calf < foot is consistent with the marked increase in peripheral vascular disease and gangrene in the lower extremities of diabetic patients.",
keywords = "acellular capillaries, capillary basement membrane width, diabetes, diabetic retinopathy, Pericyte, skeletal muscle",
author = "Ronald Tilton and Faller, {A. M.} and Burkhardt, {J. K.} and Hoffmann, {P. L.} and C. Kilo and Williamson, {J. R.}",
year = "1985",
month = "12",
doi = "10.1007/BF00703132",
language = "English (US)",
volume = "28",
pages = "895--900",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer Verlag",
number = "12",

}

TY - JOUR

T1 - Pericyte degeneration and acellular capillaries are increased in the feet of human diabetic patients

AU - Tilton, Ronald

AU - Faller, A. M.

AU - Burkhardt, J. K.

AU - Hoffmann, P. L.

AU - Kilo, C.

AU - Williamson, J. R.

PY - 1985/12

Y1 - 1985/12

N2 - Ultrastractural morphometry was used to quantify capillary basement membrane width, pericyte coverage of capillaries, pericyte degeneration, and the extent of acellular capillaries in skeletal muscle obtained at autopsy from neck, thigh, calf and foot of five male and four female diabetic subjects and an equal number of sex- and age-matched nondiabetic subjects. Within diabetic or nondiabetic subjects, the trend for all four parameters to increase in frequency or magnitude in the order neck < thigh < calf was highly significant; the only statistically significant difference between calf and foot muscles for any of the four parameters was capillary basement membrane width for nondiabetic subjects, which was significantly thinner in foot than in calf muscle (t=2.45;p<0.05). Pericyte coverage of capillaries did not differ between diabetic and nondiabetic subjects for each muscle examined; however, capillary basement membrane width, the frequency of pericyte debris and acellular capillaries were increased significantly in the lower extremity muscles of diabetic compared to nondiabetic subjects, and the magnitude of the difference between these two groups increased in the order thigh < calf < foot. The observations that pericyte degeneration and acellular capillaries are present in skeletal muscle as well as in retinal microvessels suggest that common pathophysiological mechanisms may contribute to vascular disease in these two very different tissues. The additional finding that relative differences between diabetic and nondiabetic subjects, in the frequency and magnitude of these changes, increase in the order neck < calf < foot is consistent with the marked increase in peripheral vascular disease and gangrene in the lower extremities of diabetic patients.

AB - Ultrastractural morphometry was used to quantify capillary basement membrane width, pericyte coverage of capillaries, pericyte degeneration, and the extent of acellular capillaries in skeletal muscle obtained at autopsy from neck, thigh, calf and foot of five male and four female diabetic subjects and an equal number of sex- and age-matched nondiabetic subjects. Within diabetic or nondiabetic subjects, the trend for all four parameters to increase in frequency or magnitude in the order neck < thigh < calf was highly significant; the only statistically significant difference between calf and foot muscles for any of the four parameters was capillary basement membrane width for nondiabetic subjects, which was significantly thinner in foot than in calf muscle (t=2.45;p<0.05). Pericyte coverage of capillaries did not differ between diabetic and nondiabetic subjects for each muscle examined; however, capillary basement membrane width, the frequency of pericyte debris and acellular capillaries were increased significantly in the lower extremity muscles of diabetic compared to nondiabetic subjects, and the magnitude of the difference between these two groups increased in the order thigh < calf < foot. The observations that pericyte degeneration and acellular capillaries are present in skeletal muscle as well as in retinal microvessels suggest that common pathophysiological mechanisms may contribute to vascular disease in these two very different tissues. The additional finding that relative differences between diabetic and nondiabetic subjects, in the frequency and magnitude of these changes, increase in the order neck < calf < foot is consistent with the marked increase in peripheral vascular disease and gangrene in the lower extremities of diabetic patients.

KW - acellular capillaries

KW - capillary basement membrane width

KW - diabetes

KW - diabetic retinopathy

KW - Pericyte

KW - skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=0022356499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022356499&partnerID=8YFLogxK

U2 - 10.1007/BF00703132

DO - 10.1007/BF00703132

M3 - Article

C2 - 4092858

AN - SCOPUS:0022356499

VL - 28

SP - 895

EP - 900

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 12

ER -