Peripheral and central oxidative stress in chemotherapy-induced neuropathic pain

Hyun Soo Shim, Chilman Bae, Jigong Wang, Kyung Hee Lee, Kali M. Hankerd, Hee Kee Kim, Jin Chung, Jun-Ho La

Research output: Contribution to journalArticle

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Abstract

Chemotherapy-induced peripheral neuropathy (CIPN) is an adverse side effect of many anti-cancer chemotherapeutic treatments. CIPN often causes neuropathic pain in extremities, and oxidative stress has been shown to be a major contributing factor to this pain. In this study, we determined the site of oxidative stress associated with pain (specifically, mechanical hypersensitivity) in cisplatin- and paclitaxel-treated mouse models of CIPN and investigated the neurophysiological mechanisms accounting for the pain. C57BL/6N mice that received either cisplatin or paclitaxel (2 mg/kg, once daily on four alternate days) developed mechanical hypersensitivity to von Frey filament stimulations of their hindpaws. Cisplatin-induced mechanical hypersensitivity was inhibited by silencing of Transient Receptor Potential channels V1 (TRPV1)- or TRPA1-expressing afferents, whereas paclitaxel-induced mechanical hypersensitivity was attenuated by silencing of Aβ fibers. Although systemic delivery of phenyl N-tert-butylnitrone, a reactive oxygen species scavenger, alleviated mechanical hypersensitivity in both cisplatin- and paclitaxel-treated mice, intraplantar phenyl N-tert-butylnitrone was effective only in cisplatin-treated mice, and intrathecal phenyl N-tert-butylnitrone, only in paclitaxel-treated mice. In a reactive oxygen species-dependent manner, the mechanosensitivity of Aδ/C fiber endings in the hindpaw skin was increased in cisplatin-treated mice, and the excitatory synaptic strength in the spinal dorsal horn was potentiated in paclitaxel-treated mice. Collectively, these results suggest that cisplatin-induced mechanical hypersensitivity is attributed to peripheral oxidative stress sensitizing mechanical nociceptors, whereas paclitaxel-induced mechanical hypersensitivity is due to central (spinal) oxidative stress maintaining central sensitization that abnormally produces pain in response to Aβ fiber inputs.

Original languageEnglish (US)
JournalMolecular Pain
Volume15
DOIs
StatePublished - Apr 1 2019

Fingerprint

Neuralgia
Hypersensitivity
Oxidative Stress
Drug Therapy
Paclitaxel
Cisplatin
Peripheral Nervous System Diseases
Myelinated Nerve Fibers
Pain
Reactive Oxygen Species
Central Nervous System Sensitization
Transient Receptor Potential Channels
Nociceptors
Inbred C57BL Mouse
Extremities
Skin
TP protocol
phenyl-N-tert-butylnitrone
Neoplasms

Keywords

  • chemotherapy
  • chemotherapy-induced peripheral neuropathy
  • cisplatin
  • neuropathic pain
  • Oxidative stress
  • paclitaxel

ASJC Scopus subject areas

  • Molecular Medicine
  • Cellular and Molecular Neuroscience
  • Anesthesiology and Pain Medicine

Cite this

Peripheral and central oxidative stress in chemotherapy-induced neuropathic pain. / Shim, Hyun Soo; Bae, Chilman; Wang, Jigong; Lee, Kyung Hee; Hankerd, Kali M.; Kim, Hee Kee; Chung, Jin; La, Jun-Ho.

In: Molecular Pain, Vol. 15, 01.04.2019.

Research output: Contribution to journalArticle

Shim, Hyun Soo ; Bae, Chilman ; Wang, Jigong ; Lee, Kyung Hee ; Hankerd, Kali M. ; Kim, Hee Kee ; Chung, Jin ; La, Jun-Ho. / Peripheral and central oxidative stress in chemotherapy-induced neuropathic pain. In: Molecular Pain. 2019 ; Vol. 15.
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