Peripheral blood mononuclear-cell interleukin-2 production, receptor generation and lymphokine-activated cytotoxicity in inflammatory bowel disease

R. H. Ming, D. Atluru, C. W. Spellman, T. Imir, James Goodwin, R. G. Strickland

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The interleukin-2 pathway is essential for the normal immune response to antigen stimulation; we have examined the possibility that this may underlie abnormal peripheral blood lymphocyte immunoregulatory function that has been observed in patients with inflammatory bowel disease. We studied 11 patients with Crohn's disease and 5 with ulcerative colitis, all with quiescent disease activity. Peripheral blood mononuclear cells were isolated from these patients and from healthy age- and sex-matched controls. Interleukin-2 production after mitogen and phorbol-myristate acetate stimulation was similar in both groups: 381±71 (mean ± SE) U/ml by control cells and 451±70 by patient cells. Interleukin-2 receptor generation was also measured pre- and poststimulation by labeling with anti-Tac antibody. This was 10.45±1 and 69.95±3.85% for control cells and 11.41±1.38 and 60.9±4.25% for patients cells. Finally, we examined the response of these cells to interleukin-2 stimulation by generating cells with direct cytotoxicity to51Cr-labeled Daudi-cell targets. Control cells caused 59.5±46%51Cr release, whereas patient cells caused 50.8±5.18% release. None of the above results achieved statistical significance. We conclude that the peripheral blood interleukin-2 pathway is normal in inactive inflammatory bowel disease.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalJournal of Clinical Immunology
Volume7
Issue number1
DOIs
StatePublished - Jan 1987
Externally publishedYes

Fingerprint

Lymphokines
Interleukin-2 Receptors
Inflammatory Bowel Diseases
Blood Cells
Interleukin-2
Histocompatibility Antigens Class II
Tetradecanoylphorbol Acetate
Mitogens
Ulcerative Colitis
Crohn Disease
Anti-Idiotypic Antibodies
Lymphocytes

Keywords

  • Inflammatory bowel disease
  • interleukin-2
  • peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Peripheral blood mononuclear-cell interleukin-2 production, receptor generation and lymphokine-activated cytotoxicity in inflammatory bowel disease. / Ming, R. H.; Atluru, D.; Spellman, C. W.; Imir, T.; Goodwin, James; Strickland, R. G.

In: Journal of Clinical Immunology, Vol. 7, No. 1, 01.1987, p. 59-63.

Research output: Contribution to journalArticle

@article{cc39d2b038324cceb5d8f2d933c0b017,
title = "Peripheral blood mononuclear-cell interleukin-2 production, receptor generation and lymphokine-activated cytotoxicity in inflammatory bowel disease",
abstract = "The interleukin-2 pathway is essential for the normal immune response to antigen stimulation; we have examined the possibility that this may underlie abnormal peripheral blood lymphocyte immunoregulatory function that has been observed in patients with inflammatory bowel disease. We studied 11 patients with Crohn's disease and 5 with ulcerative colitis, all with quiescent disease activity. Peripheral blood mononuclear cells were isolated from these patients and from healthy age- and sex-matched controls. Interleukin-2 production after mitogen and phorbol-myristate acetate stimulation was similar in both groups: 381±71 (mean ± SE) U/ml by control cells and 451±70 by patient cells. Interleukin-2 receptor generation was also measured pre- and poststimulation by labeling with anti-Tac antibody. This was 10.45±1 and 69.95±3.85{\%} for control cells and 11.41±1.38 and 60.9±4.25{\%} for patients cells. Finally, we examined the response of these cells to interleukin-2 stimulation by generating cells with direct cytotoxicity to51Cr-labeled Daudi-cell targets. Control cells caused 59.5±46{\%}51Cr release, whereas patient cells caused 50.8±5.18{\%} release. None of the above results achieved statistical significance. We conclude that the peripheral blood interleukin-2 pathway is normal in inactive inflammatory bowel disease.",
keywords = "Inflammatory bowel disease, interleukin-2, peripheral blood mononuclear cells",
author = "Ming, {R. H.} and D. Atluru and Spellman, {C. W.} and T. Imir and James Goodwin and Strickland, {R. G.}",
year = "1987",
month = "1",
doi = "10.1007/BF00915426",
language = "English (US)",
volume = "7",
pages = "59--63",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Peripheral blood mononuclear-cell interleukin-2 production, receptor generation and lymphokine-activated cytotoxicity in inflammatory bowel disease

AU - Ming, R. H.

AU - Atluru, D.

AU - Spellman, C. W.

AU - Imir, T.

AU - Goodwin, James

AU - Strickland, R. G.

PY - 1987/1

Y1 - 1987/1

N2 - The interleukin-2 pathway is essential for the normal immune response to antigen stimulation; we have examined the possibility that this may underlie abnormal peripheral blood lymphocyte immunoregulatory function that has been observed in patients with inflammatory bowel disease. We studied 11 patients with Crohn's disease and 5 with ulcerative colitis, all with quiescent disease activity. Peripheral blood mononuclear cells were isolated from these patients and from healthy age- and sex-matched controls. Interleukin-2 production after mitogen and phorbol-myristate acetate stimulation was similar in both groups: 381±71 (mean ± SE) U/ml by control cells and 451±70 by patient cells. Interleukin-2 receptor generation was also measured pre- and poststimulation by labeling with anti-Tac antibody. This was 10.45±1 and 69.95±3.85% for control cells and 11.41±1.38 and 60.9±4.25% for patients cells. Finally, we examined the response of these cells to interleukin-2 stimulation by generating cells with direct cytotoxicity to51Cr-labeled Daudi-cell targets. Control cells caused 59.5±46%51Cr release, whereas patient cells caused 50.8±5.18% release. None of the above results achieved statistical significance. We conclude that the peripheral blood interleukin-2 pathway is normal in inactive inflammatory bowel disease.

AB - The interleukin-2 pathway is essential for the normal immune response to antigen stimulation; we have examined the possibility that this may underlie abnormal peripheral blood lymphocyte immunoregulatory function that has been observed in patients with inflammatory bowel disease. We studied 11 patients with Crohn's disease and 5 with ulcerative colitis, all with quiescent disease activity. Peripheral blood mononuclear cells were isolated from these patients and from healthy age- and sex-matched controls. Interleukin-2 production after mitogen and phorbol-myristate acetate stimulation was similar in both groups: 381±71 (mean ± SE) U/ml by control cells and 451±70 by patient cells. Interleukin-2 receptor generation was also measured pre- and poststimulation by labeling with anti-Tac antibody. This was 10.45±1 and 69.95±3.85% for control cells and 11.41±1.38 and 60.9±4.25% for patients cells. Finally, we examined the response of these cells to interleukin-2 stimulation by generating cells with direct cytotoxicity to51Cr-labeled Daudi-cell targets. Control cells caused 59.5±46%51Cr release, whereas patient cells caused 50.8±5.18% release. None of the above results achieved statistical significance. We conclude that the peripheral blood interleukin-2 pathway is normal in inactive inflammatory bowel disease.

KW - Inflammatory bowel disease

KW - interleukin-2

KW - peripheral blood mononuclear cells

UR - http://www.scopus.com/inward/record.url?scp=0023132376&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023132376&partnerID=8YFLogxK

U2 - 10.1007/BF00915426

DO - 10.1007/BF00915426

M3 - Article

VL - 7

SP - 59

EP - 63

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

SN - 0271-9142

IS - 1

ER -