Peripheral corticotropin releasing hormone mediates post-inflammatory visceral hypersensitivity in rats

Jun-Ho La, Tae Sik Sung, Hyun Ju Kim, Tae Wan Kim, Tong Mook Kang, Il Suk Yang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aim: To investigate whether peripheral corticotropin releasing hormone (CRH), which is up-regulated in intestinal inflammation, mediates the post-inflammatory visceral hypersensitivity in a rat model of colitis. Methods: We measured mucosal myeloperoxidase (MPO) activity as a marker of inflammation, plasma CRH level, and abdominal withdrawal reflex (AWR) to colorectal distension as a visceral nociceptive response at 2, 7 and 14 d after the induction of colitis with 4% acetic acid. Results: Colonic inflammation, quantified by MPO activity, significantly increased on d 2 and subsided thereafter, which indicated a resolution of inflammation within 7 d. On the contrary, plasma CRH level and AWR score were increased on d 2, remained high on d 7, and returned to control level on d 14. Intraperitoneal injection of a CRH antagonist, astressin (30 μg/kg), significantly attenuated the post-inflammatory visceral hypersensitivity on d 7. Furthermore, intraperitoneal administration of CRH (3 and 10 μg/kg) mimicked the post-inflammatory visceral hypersensitivity in naive rats. Conclusion: These results suggest that increased peripheral CRH mediates the enhanced visceral nociception in rats recovered from experimental colitis.

Original languageEnglish (US)
Pages (from-to)731-736
Number of pages6
JournalWorld Journal of Gastroenterology
Volume14
Issue number5
DOIs
StatePublished - Feb 7 2008
Externally publishedYes

Fingerprint

Corticotropin-Releasing Hormone
Hypersensitivity
Abdominal Reflex
Colitis
Inflammation
Peroxidase
Hormone Antagonists
Nociception
Intraperitoneal Injections
Acetic Acid

Keywords

  • Colitis
  • Corticotropin releasing hormone
  • Visceral hypersensitivity

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Peripheral corticotropin releasing hormone mediates post-inflammatory visceral hypersensitivity in rats. / La, Jun-Ho; Sung, Tae Sik; Kim, Hyun Ju; Kim, Tae Wan; Kang, Tong Mook; Yang, Il Suk.

In: World Journal of Gastroenterology, Vol. 14, No. 5, 07.02.2008, p. 731-736.

Research output: Contribution to journalArticle

La, Jun-Ho ; Sung, Tae Sik ; Kim, Hyun Ju ; Kim, Tae Wan ; Kang, Tong Mook ; Yang, Il Suk. / Peripheral corticotropin releasing hormone mediates post-inflammatory visceral hypersensitivity in rats. In: World Journal of Gastroenterology. 2008 ; Vol. 14, No. 5. pp. 731-736.
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