Peripheral glutamate release in the hindpaw following low and high intensity sciatic stimulation

John DeGroot, Shengtai Zhou, Susan M. Carlton

Research output: Contribution to journalArticlepeer-review

159 Scopus citations


The present study demonstrates that following A and/or C fiber stimulation of the sciatic nerve, glutamate levels increase significantly in the hindpaw extracellular space. In hindpaw dialysate, electrical stimulation (5 min) of the sciatic nerve at 2X, 20X, 50X or 200X threshold current required to produce a muscle twitch resulted in peak glutamate increases of 120.8 ± 9%, 134.3 ± 5%, 153.9 ± 10% and 150.5 ± 5% of basal levels, respectively. Application of 1% capsaicin to the sciatic nerve (10 min) to selectively activate C fibers resulted in a peak glutamate increase of 130.8 ± 8% of basal levels. Aspartate levels did not change significantly in either paradigm. These data indicate that low and high intensity stimulation can result in peripheral release of glutamate, providing a major source of ligand for the glutamate receptors localized on peripheral primary afferents. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish (US)
Pages (from-to)497-502
Number of pages6
Issue number3
StatePublished - 2000
Externally publishedYes


  • Capsaicin
  • Electrical stimulation
  • Microdialysis
  • Nociception
  • Primary afferent

ASJC Scopus subject areas

  • General Neuroscience


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