Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties

C. J. Orihuela, R. Janssen, C. W. Robb, D. A. Watson, D. W. Niesel

    Research output: Contribution to journalArticlepeer-review

    17 Scopus citations

    Abstract

    We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.

    Original languageEnglish (US)
    Pages (from-to)6082-6086
    Number of pages5
    JournalInfection and immunity
    Volume68
    Issue number10
    DOIs
    StatePublished - 2000

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

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