Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties

C. J. Orihuela, R. Janssen, C. W. Robb, D. A. Watson, David Niesel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.

Original languageEnglish (US)
Pages (from-to)6082-6086
Number of pages5
JournalInfection and Immunity
Volume68
Issue number10
DOIs
StatePublished - 2000

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Streptococcus pneumoniae
Virulence
Peritoneal Cavity
Virulence Factors
Serum
Proteins
Gels
Epithelial Cells
Messenger RNA
In Vitro Techniques
A549 Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties. / Orihuela, C. J.; Janssen, R.; Robb, C. W.; Watson, D. A.; Niesel, David.

In: Infection and Immunity, Vol. 68, No. 10, 2000, p. 6082-6086.

Research output: Contribution to journalArticle

Orihuela, C. J. ; Janssen, R. ; Robb, C. W. ; Watson, D. A. ; Niesel, David. / Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties. In: Infection and Immunity. 2000 ; Vol. 68, No. 10. pp. 6082-6086.
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