Permeabilization of lipid bilayers is a common conformation-dependent activity of soluble amyloid oligomers in protein misfolding diseases

Rakez Kayed, Yuri Sokolov, Brian Edmonds, Theresa M. McIntire, Saskia C. Milton, James E. Hall, Charles G. Glabe

Research output: Contribution to journalArticle

669 Citations (Scopus)

Abstract

Amyloid fibrillization is multistep process involving soluble oligomeric intermediates, including spherical oligomers and protofibrils. Amyloid oligomers have a common, generic structure, and they are intrinsically toxic to cells, even when formed from non-disease related proteins, which implies they also share a common mechanism of pathogenesis and toxicity. Here we report that soluble oligomers from several types of amyloids specifically increase lipid bilayer conductance regardless of the sequence, while fibrils and soluble low molecular weight species have no effect. The increase in membrane conductance occurs without any evidence of discrete channel or pore formation or ion selectivity. The conductance is dependent on the concentration of oligomers and can be reversed by anti-oligomer antibody. These results indicate that soluble oligomers from many types of amyloidogenic proteins and peptides increase membrane conductance in a conformation-specific fashion and suggest that this may represent the common primary mechanism of pathogenesis in amyloid-related degenerative diseases.

Original languageEnglish (US)
Pages (from-to)46363-46366
Number of pages4
JournalJournal of Biological Chemistry
Volume279
Issue number45
DOIs
StatePublished - Nov 5 2004
Externally publishedYes

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Proteostasis Deficiencies
Lipid bilayers
Lipid Bilayers
Oligomers
Amyloid
Conformations
Proteins
Amyloidogenic Proteins
Membranes
Poisons
Anti-Idiotypic Antibodies
Molecular Weight
Ions
Peptides
Toxicity
Molecular weight
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Permeabilization of lipid bilayers is a common conformation-dependent activity of soluble amyloid oligomers in protein misfolding diseases. / Kayed, Rakez; Sokolov, Yuri; Edmonds, Brian; McIntire, Theresa M.; Milton, Saskia C.; Hall, James E.; Glabe, Charles G.

In: Journal of Biological Chemistry, Vol. 279, No. 45, 05.11.2004, p. 46363-46366.

Research output: Contribution to journalArticle

Kayed, Rakez ; Sokolov, Yuri ; Edmonds, Brian ; McIntire, Theresa M. ; Milton, Saskia C. ; Hall, James E. ; Glabe, Charles G. / Permeabilization of lipid bilayers is a common conformation-dependent activity of soluble amyloid oligomers in protein misfolding diseases. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 45. pp. 46363-46366.
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