Peroxynitrite Causes Energy Depletion and Increases Permeability via Activation of Poly (ADP-Ribose) Synthetase in Pulmonary Epithelial Cells

Csaba Szabo, Christine Saunders, Michael O'Connor, Andrew L. Salzman

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Recent studies show that peroxynitrite is a potent trigger of DNA strand breakage, which in turn activates the nuclear repair enzyme poly (ADP-ribose) synthetase (PARS), resulting in a cellular energy deficit. Here we present evidence that treatment of A549 human pulmonary epithelial cells with peroxynitrite (1 mM) results in ADP-ribosylation, NAD+ depletion, inhibition of mitochondrial respiration, and increased epithelial paracellular permeability. The PARS inhibitor 3-aminobenzamide (1 mM) provided a significant, partial protection against the energetic and functional changes. Similarly, inhibition of PARS activity by 3-aminobenzamide reduced the peroxynitrite-induced suppression of mitochondrial respiration in BEAS-2B human bronchial epithelial cells. Thus, PARS activation and energy depletion represents one of the pathways of peroxynitrite-mediated epithelial toxicity. Inhibition of PARS may improve cellular energy homeostasis in pathophysiologic conditions associated with peroxynitrite generation.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume16
Issue number2
StatePublished - 1997
Externally publishedYes

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Poly Adenosine Diphosphate Ribose
Peroxynitrous Acid
Ligases
Permeability
Epithelial Cells
Chemical activation
Lung
Respiration
NAD
Adenosine Diphosphate
Toxicity
Homeostasis
Repair
DNA
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Peroxynitrite Causes Energy Depletion and Increases Permeability via Activation of Poly (ADP-Ribose) Synthetase in Pulmonary Epithelial Cells. / Szabo, Csaba; Saunders, Christine; O'Connor, Michael; Salzman, Andrew L.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 16, No. 2, 1997, p. 105-109.

Research output: Contribution to journalArticle

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