Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995

L. L. Rodriguez, A. De Roo, Y. Guimard, S. G. Trappier, A. Sanchez, D. Bressler, A. J. Williams, A. K. Rowe, J. Bertolli, A. S. Khan, Thomas Ksiazek, C. J. Peters, Stuart T. Nichol

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Abstract

Ebola virus persistence was examined in body fluids from 12 convalescent patients by virus isolation and reverse transcription-polymerase chain reaction (RT-PCR) during the 1995 Ebola hemorrhagic fever outbreak in Kikwit, Democratic Republic of the Congo. Virus RNA could be detected for up to 33 days in vaginal, rectal, and conjunctival swabs of 1 patient and up to 101 days in the seminal fluid of 4 patients. Infectious virus was detected in 1 seminal fluid sample obtained 82 days after disease onset. Sequence analysis of an RT-PCR fragment of the most variable region of the glycoprotein gene amplified from 9 patients revealed no nucleotide changes. The patient samples were selected so that they would include some from a suspected line of transmission with at least three human-to-human passages, some from 5 survivors and 4 deceased patients, and 2 from patients who provided multiple samples through convalescence. There was no evidence of different virus variants cocirculating during the outbreak or of genetic variation accumulating during human-to-human passage or during prolonged persistence in individual patients.

Original languageEnglish (US)
JournalJournal of Infectious Diseases
Volume179
Issue numberSUPPL. 1
StatePublished - 1999
Externally publishedYes

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Ebolavirus
Democratic Republic of the Congo
Disease Outbreaks
Viruses
Reverse Transcription
Ebola Hemorrhagic Fever
Patient Isolation
Polymerase Chain Reaction
RNA Viruses
Body Fluids
Sequence Analysis
Survivors
Glycoproteins
Nucleotides

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Rodriguez, L. L., De Roo, A., Guimard, Y., Trappier, S. G., Sanchez, A., Bressler, D., ... Nichol, S. T. (1999). Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995. Journal of Infectious Diseases, 179(SUPPL. 1).

Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995. / Rodriguez, L. L.; De Roo, A.; Guimard, Y.; Trappier, S. G.; Sanchez, A.; Bressler, D.; Williams, A. J.; Rowe, A. K.; Bertolli, J.; Khan, A. S.; Ksiazek, Thomas; Peters, C. J.; Nichol, Stuart T.

In: Journal of Infectious Diseases, Vol. 179, No. SUPPL. 1, 1999.

Research output: Contribution to journalArticle

Rodriguez, LL, De Roo, A, Guimard, Y, Trappier, SG, Sanchez, A, Bressler, D, Williams, AJ, Rowe, AK, Bertolli, J, Khan, AS, Ksiazek, T, Peters, CJ & Nichol, ST 1999, 'Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995', Journal of Infectious Diseases, vol. 179, no. SUPPL. 1.
Rodriguez LL, De Roo A, Guimard Y, Trappier SG, Sanchez A, Bressler D et al. Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995. Journal of Infectious Diseases. 1999;179(SUPPL. 1).
Rodriguez, L. L. ; De Roo, A. ; Guimard, Y. ; Trappier, S. G. ; Sanchez, A. ; Bressler, D. ; Williams, A. J. ; Rowe, A. K. ; Bertolli, J. ; Khan, A. S. ; Ksiazek, Thomas ; Peters, C. J. ; Nichol, Stuart T. / Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995. In: Journal of Infectious Diseases. 1999 ; Vol. 179, No. SUPPL. 1.
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