Persistent infection with mouse hepatitis virus, JHM strain in DBT cell culture.

N. Hirano, N. Goto, Shinji Makino, K. Fujiwara

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

After inoculation with JHM strain into DBT cell monolayers, a persistently infected DBT cell culture was established without producing typical cytopathic changes after about 15th passages. By immunofluorescence virus specific antigen was demonstrated in 10 to 15% DBT cells. This persistently infected culture (JHM-CC) was resistant to superinfection with parental JHM, but such resistance was not shown against vesicular stomatitis virus. JHM-CC virus produced small plaques on DBT cell monolayers. Temperature sensitive (TS) mutant, defective interfering (DI) particle or interferon was not detected in the JHM-CC. To intracerebral inoculation with JHM-CC virus, cortisone treated ICR mice survived without showing clinical signs, however, demyelinating lesions were produced in the brain and spinal cord of them.

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalAdvances in Experimental Medicine and Biology
Volume142
StatePublished - 1981
Externally publishedYes

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Murine hepatitis virus
Viruses
Cell culture
Cell Culture Techniques
Infection
Monolayers
Defective Viruses
Inbred ICR Mouse
Superinfection
Vesicular Stomatitis
Cortisone
Interferons
Fluorescent Antibody Technique
Brain
Spinal Cord
Antigens
Temperature

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Persistent infection with mouse hepatitis virus, JHM strain in DBT cell culture. / Hirano, N.; Goto, N.; Makino, Shinji; Fujiwara, K.

In: Advances in Experimental Medicine and Biology, Vol. 142, 1981, p. 301-308.

Research output: Contribution to journalArticle

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