Persistent West Nile Virus infection in the golden hamster

Studies on its mechanism and possible implications for other flavivirus infections

Robert B. Tesh, Marina Siirin, Hilda Guzman, Amelia P A Travassos Da Rosa, Xiaoyan Wu, Tao Duan, Hao Lei, Marcio R. Nunes, Shu Yuan Xiao

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Golden hamsters (Mesocricetus auratus) experimentally infected with West Nile virus (WNV) developed chronic renal infection and persistent shedding of virus in urine for up to 8 months, despite initial rapid clearance of virus from blood and the timely appearance of high levels of specific neutralizing antibodies. Infectious WNV could be recovered by direct culture of their urine and by cocultivation of kidney tissue for up to 247 days after initial infection. Only moderate histopathologic changes were observed in the kidneys or brain of the chronically infected hamsters, although WNV antigen was readily detected by immunohistochemistry within epithelium, interstitial cells, and macrophages in the distal renal tubules. Comparison of WNV isolates from serial urine samples from individual hamsters over several months indicated that the virus underwent both genetic and phenotypic changes during persistent infection. These findings are similar to previous reports of persistent infection with tickborne encephalitis and Modoc viruses.

Original languageEnglish (US)
Pages (from-to)287-295
Number of pages9
JournalJournal of Infectious Diseases
Volume192
Issue number2
DOIs
StatePublished - Jul 15 2005

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Flavivirus Infections
West Nile virus
Mesocricetus
Virus Diseases
Urine
Infection
Kidney
Cricetinae
Distal Kidney Tubule
Encephalitis Viruses
Viruses
Virus Shedding
Coculture Techniques
Neutralizing Antibodies
Epithelium
Immunohistochemistry
Macrophages
Antigens
Brain

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Persistent West Nile Virus infection in the golden hamster : Studies on its mechanism and possible implications for other flavivirus infections. / Tesh, Robert B.; Siirin, Marina; Guzman, Hilda; Travassos Da Rosa, Amelia P A; Wu, Xiaoyan; Duan, Tao; Lei, Hao; Nunes, Marcio R.; Xiao, Shu Yuan.

In: Journal of Infectious Diseases, Vol. 192, No. 2, 15.07.2005, p. 287-295.

Research output: Contribution to journalArticle

Tesh, RB, Siirin, M, Guzman, H, Travassos Da Rosa, APA, Wu, X, Duan, T, Lei, H, Nunes, MR & Xiao, SY 2005, 'Persistent West Nile Virus infection in the golden hamster: Studies on its mechanism and possible implications for other flavivirus infections', Journal of Infectious Diseases, vol. 192, no. 2, pp. 287-295. https://doi.org/10.1086/431153
Tesh, Robert B. ; Siirin, Marina ; Guzman, Hilda ; Travassos Da Rosa, Amelia P A ; Wu, Xiaoyan ; Duan, Tao ; Lei, Hao ; Nunes, Marcio R. ; Xiao, Shu Yuan. / Persistent West Nile Virus infection in the golden hamster : Studies on its mechanism and possible implications for other flavivirus infections. In: Journal of Infectious Diseases. 2005 ; Vol. 192, No. 2. pp. 287-295.
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