Pharmacological tools for hydrogen sulphide research: A brief, introductory guide for beginners

The purpose of this brief review is to help researchers in their initial approach to the H₂S field and to provide answers for the most frequently posed questions by newcomers to the topic related to H₂S donors and inhibitors of H₂S synthesis, as well as methods to measure H₂S production. Here the reader will find a practical guide that provides fast and to the point information on how to (i) deliver H₂S to cells; (ii) modulate its endogenous production; and (iii) measure its levels in fluids, cells and tissues in order to gain an understanding of its role in health and disease.