PharmaSat: Drug dose response in microgravity from a free-flying integrated biofluidic/optical culture-and-analysis satellite

Antonio J. Ricco, MacArena Parra, David Niesel, Matthew Piccini, Diana Ly, Michael McGinnis, Andrzej Kudlicki, John W. Hines, Linda Timucin, Chris Beasley, Robert Ricks, Michael McIntyre, Charlie Friedericks, Michael Henschke, Ricky Leung, Millan Diaz-Aguado, Christopher Kitts, Ignacio Mas, Mike Rasay, Elwood AgasidEd Luzzi, Karolyn Ronzano, David Squires, Bruce Yost

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Scopus citations

Abstract

We designed, built, tested, space-qualified, launched, and collected telemetered data from low Earth orbit from Pharma- Sat, a 5.1-kg free flying "nanosatellite" that supported microbial growth in 48 microfluidic wells, dosed microbes with multiple concentrations of a pharmaceutical agent, and monitored microbial growth and metabolic activity using a dedicated 3-color optical absorbance system at each microwell. The PharmaSat nanosatellite comprised a structure approximately 10 x 10 x 35 cm, including triple-junction solar cells, bidirectional communications, power-generation and energy- storage system, and a sealed payload 1.2-L containment vessel that housed the biological organisms along with the fluidic, optical, thermal, sensor, and electronic subsystems. Growth curves for S. cerevisiae (Brewer's yeast) were obtained for multiple concentrations of the antifungal drug voriconazole in the microgravity conditions of low Earth orbit. Corresponding terrestrial control experiments were conducted for comparison.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume7929
DOIs
StatePublished - 2011
EventMicrofluidics, BioMEMS, and Medical Microsystems IX - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems IX
CountryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

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Keywords

  • integrated microsystem
  • microfluidics
  • nanosatellite
  • optical absorbance
  • PharmaSat
  • voriconazole
  • yeast

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Ricco, A. J., Parra, M., Niesel, D., Piccini, M., Ly, D., McGinnis, M., Kudlicki, A., Hines, J. W., Timucin, L., Beasley, C., Ricks, R., McIntyre, M., Friedericks, C., Henschke, M., Leung, R., Diaz-Aguado, M., Kitts, C., Mas, I., Rasay, M., ... Yost, B. (2011). PharmaSat: Drug dose response in microgravity from a free-flying integrated biofluidic/optical culture-and-analysis satellite. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 7929). [79290T] https://doi.org/10.1117/12.881082