Abstract
A thorough phenotypic characterization of yellow fever (YF) virus generated from cDNA is a necessary prerequisite for mapping virulence/attenuation determinants and exploring the potential of YF attenuated virus 17D as a carrier for heterologous protective epitopes. In this study, YF virus was produced from 17D cDNA clones after lipofectin- mediated RNA transfection of certified primary cultures of chicken embryo fibroblasts (YFiv5.2/SL). This virus was passaged once in embryonated chicken eggs according to current YF vaccine manufacture methodology to produce the experimental virus (YFiv5.2/VL). These viruses were characterized in established monkey neurovirulence safety tests and quantitative clinical and histologic scores were derived for each virus. The experimental vaccine viruses (YFiv5.2/SL and VL) compared favorably with another well-known YF vaccine strain (17DD) used as control virus for the histologic score. Although slightly higher clinical neurovirulence was observed for YFiv5.2 as compared with the 17DD virus, it should not preclude the use of YFiv5.2 for mapping YF virus virulence determinants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 75-80 |
| Number of pages | 6 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 52 |
| Issue number | 1 |
| State | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Phenotypic analysis of yellow fever virus derived from complementary DNA. / Marchevsky, R. S.; Mariano, J.; Ferreira, V. S.; Almeida, E.; Cerqueira, M. J.; Carvalho, R.; Pissurno, J. W.; Travassos da Rosa, A. P A; Simoes, M. C.; Santos, C. N D; Ferreira, I. I.; Muylaert, I. R.; Mann, G. F.; Rice, C. M.; Galler, R.
In: American Journal of Tropical Medicine and Hygiene, Vol. 52, No. 1, 1995, p. 75-80.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenotypic analysis of yellow fever virus derived from complementary DNA
AU - Marchevsky, R. S.
AU - Mariano, J.
AU - Ferreira, V. S.
AU - Almeida, E.
AU - Cerqueira, M. J.
AU - Carvalho, R.
AU - Pissurno, J. W.
AU - Travassos da Rosa, A. P A
AU - Simoes, M. C.
AU - Santos, C. N D
AU - Ferreira, I. I.
AU - Muylaert, I. R.
AU - Mann, G. F.
AU - Rice, C. M.
AU - Galler, R.
PY - 1995
Y1 - 1995
N2 - A thorough phenotypic characterization of yellow fever (YF) virus generated from cDNA is a necessary prerequisite for mapping virulence/attenuation determinants and exploring the potential of YF attenuated virus 17D as a carrier for heterologous protective epitopes. In this study, YF virus was produced from 17D cDNA clones after lipofectin- mediated RNA transfection of certified primary cultures of chicken embryo fibroblasts (YFiv5.2/SL). This virus was passaged once in embryonated chicken eggs according to current YF vaccine manufacture methodology to produce the experimental virus (YFiv5.2/VL). These viruses were characterized in established monkey neurovirulence safety tests and quantitative clinical and histologic scores were derived for each virus. The experimental vaccine viruses (YFiv5.2/SL and VL) compared favorably with another well-known YF vaccine strain (17DD) used as control virus for the histologic score. Although slightly higher clinical neurovirulence was observed for YFiv5.2 as compared with the 17DD virus, it should not preclude the use of YFiv5.2 for mapping YF virus virulence determinants.
AB - A thorough phenotypic characterization of yellow fever (YF) virus generated from cDNA is a necessary prerequisite for mapping virulence/attenuation determinants and exploring the potential of YF attenuated virus 17D as a carrier for heterologous protective epitopes. In this study, YF virus was produced from 17D cDNA clones after lipofectin- mediated RNA transfection of certified primary cultures of chicken embryo fibroblasts (YFiv5.2/SL). This virus was passaged once in embryonated chicken eggs according to current YF vaccine manufacture methodology to produce the experimental virus (YFiv5.2/VL). These viruses were characterized in established monkey neurovirulence safety tests and quantitative clinical and histologic scores were derived for each virus. The experimental vaccine viruses (YFiv5.2/SL and VL) compared favorably with another well-known YF vaccine strain (17DD) used as control virus for the histologic score. Although slightly higher clinical neurovirulence was observed for YFiv5.2 as compared with the 17DD virus, it should not preclude the use of YFiv5.2 for mapping YF virus virulence determinants.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028917805&partnerID=8YFLogxK
M3 - Article
VL - 52
SP - 75
EP - 80
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 1
ER -