Phenotypic analysis of yellow fever virus derived from complementary DNA

R. S. Marchevsky; J. Mariano; V. S. Ferreira; E. Almeida; M. J. Cerqueira; R. Carvalho; J. W. Pissurno; A. P.A. Travassos da Rosa; M. C. Simoes; C. N.D. Santos; I. I. Ferreira; I. R. Muylaert; G. F. Mann; C. M. Rice; R. Galler

doi:10.4269/ajtmh.1995.52.75

Phenotypic analysis of yellow fever virus derived from complementary DNA

R. S. Marchevsky, J. Mariano, V. S. Ferreira, E. Almeida, M. J. Cerqueira, R. Carvalho, J. W. Pissurno, A. P.A. Travassos da Rosa, M. C. Simoes, C. N.D. Santos, I. I. Ferreira, I. R. Muylaert, G. F. Mann, C. M. Rice, R. Galler

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A thorough phenotypic characterization of yellow fever (YF) virus generated from cDNA is a necessary prerequisite for mapping virulence/attenuation determinants and exploring the potential of YF attenuated virus 17D as a carrier for heterologous protective epitopes. In this study, YF virus was produced from 17D cDNA clones after lipofectin- mediated RNA transfection of certified primary cultures of chicken embryo fibroblasts (YFiv5.2/SL). This virus was passaged once in embryonated chicken eggs according to current YF vaccine manufacture methodology to produce the experimental virus (YFiv5.2/VL). These viruses were characterized in established monkey neurovirulence safety tests and quantitative clinical and histologic scores were derived for each virus. The experimental vaccine viruses (YFiv5.2/SL and VL) compared favorably with another well-known YF vaccine strain (17DD) used as control virus for the histologic score. Although slightly higher clinical neurovirulence was observed for YFiv5.2 as compared with the 17DD virus, it should not preclude the use of YFiv5.2 for mapping YF virus virulence determinants.

Original language	English (US)
Pages (from-to)	75-80
Number of pages	6
Journal	American Journal of Tropical Medicine and Hygiene
Volume	52
Issue number	1
DOIs	https://doi.org/10.4269/ajtmh.1995.52.75
State	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Virology
Infectious Diseases

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10.4269/ajtmh.1995.52.75

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Marchevsky, R. S., Mariano, J., Ferreira, V. S., Almeida, E., Cerqueira, M. J., Carvalho, R., Pissurno, J. W., Travassos da Rosa, A. P. A., Simoes, M. C., Santos, C. N. D., Ferreira, I. I., Muylaert, I. R., Mann, G. F., Rice, C. M., & Galler, R. (1995). Phenotypic analysis of yellow fever virus derived from complementary DNA. American Journal of Tropical Medicine and Hygiene, 52(1), 75-80. https://doi.org/10.4269/ajtmh.1995.52.75

Marchevsky, RS, Mariano, J, Ferreira, VS, Almeida, E, Cerqueira, MJ, Carvalho, R, Pissurno, JW, Travassos da Rosa, APA, Simoes, MC, Santos, CND, Ferreira, II, Muylaert, IR, Mann, GF, Rice, CM & Galler, R 1995, 'Phenotypic analysis of yellow fever virus derived from complementary DNA', American Journal of Tropical Medicine and Hygiene, vol. 52, no. 1, pp. 75-80. https://doi.org/10.4269/ajtmh.1995.52.75

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abstract = "A thorough phenotypic characterization of yellow fever (YF) virus generated from cDNA is a necessary prerequisite for mapping virulence/attenuation determinants and exploring the potential of YF attenuated virus 17D as a carrier for heterologous protective epitopes. In this study, YF virus was produced from 17D cDNA clones after lipofectin- mediated RNA transfection of certified primary cultures of chicken embryo fibroblasts (YFiv5.2/SL). This virus was passaged once in embryonated chicken eggs according to current YF vaccine manufacture methodology to produce the experimental virus (YFiv5.2/VL). These viruses were characterized in established monkey neurovirulence safety tests and quantitative clinical and histologic scores were derived for each virus. The experimental vaccine viruses (YFiv5.2/SL and VL) compared favorably with another well-known YF vaccine strain (17DD) used as control virus for the histologic score. Although slightly higher clinical neurovirulence was observed for YFiv5.2 as compared with the 17DD virus, it should not preclude the use of YFiv5.2 for mapping YF virus virulence determinants.",

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AU - Travassos da Rosa, A. P.A.

AU - Simoes, M. C.

AU - Santos, C. N.D.

AU - Ferreira, I. I.

AU - Muylaert, I. R.

AU - Mann, G. F.

AU - Rice, C. M.

AU - Galler, R.

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Phenotypic analysis of yellow fever virus derived from complementary DNA

Abstract

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